Analysis of Fat-Soluble Vitamins. XXIV. High Performance Liquid Chromatographic Determination of Vitamin D in Vitamin D Resin Containing Powders: Collaborative Study1

Abstract

Further study of vitamin D methodology solved the discrepancy between the AOAC chemical method, 43.068-43.078, and the HPLC assay for vitamin D3 in resin containing dry powders. The discrepancy is caused by the difference in solubility of the vitamin D3 resin in benzene and in pentane. The method has been modified accordingly, and has been adopted official first action for vitamin D3 resins and vitamin D3 resin containing powders and aqueous dispersions.