Analysis of expressed sequence tags derived from pea leaves infected by Peronospora viciae f. sp. pisi

Abstract

A cDNA library was constructed from field pea leaves infected by the downy mildew pathogen, Peronospora viciae f. sp. pisi, using a suppression subtractive hybridisation approach. The library consists of 399 expressed sequence tags, from which 207 unisequences were obtained after sequence assembly. Of the unisequences, six were shown to be of Peronospora viciae f. sp. pisi origin. The remaining unisequences were subjected to gene ontology analysis and their functions were predicted in silico. Eleven of these unisequences (representing 24 clones) shared significant sequence similarities with Arabidopsis genes known to be involved in downy mildew resistance, including the well‐characterised genes RPP5, RPP6 and RPP27. Expression analysis of five selected unisequences by real‐time PCR indicated that all five were up‐regulated during downy mildew pathogenesis, suggesting a significant role for these genes in the host response to downy mildew infection.

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

“…As a result, many studies performed on M. truncatula, soybean, pea, chickpea, peanuts, and common bean provided insight into the molecular basis of their resistance to several legume pathogens including Fusarium oxysporum, Aphanomyces euteiches, Phytophthora sojae, Didymella pinodes, Ascochyta rabiei, Phakopsora pachyrhizi, Peronospora viciae f.sp. pisi, pea seed-borne mosaic virus, bean common mosaic virus, common bacterial blight, Orobanche crenata, Phelipanche aegyptiaca, Heterodera goettingiana, Spodoptera exigua or Helicoverpa armigera (Nyamsuren et al, 2003;Gao et al, 2004;Cho et al, 2005;Cadle-Davidson and Jahn, 2006;Nimbalkar et al, 2006;Die et al, 2007;Choi et al, 2008;Darwish et al, 2008;Singh et al, 2008;Gupta et al, 2009Gupta et al, , 2010Hiraoka et al, 2009;Soria-Guerra et al, 2010;Shi et al, 2011;Veronico et al, 2011;Feng et al, 2012;Huang et al, 2012a,b;Jaiswal et al, 2012;Xu et al, 2012;Rispail et al, 2013;Toyoda et al, 2013).…”

Genomics of Plant Disease Resistance in Legumes

“…The interactions of downy mildew pathogen, Peranospora viciae f. sp. Pisi , with pea leaves were investigated with SSH cDNA libraries (Feng et al, 2012). The study identified downy mildew resistance genes RPP6/6/27 involved in this interaction.…”