Analysis of Exosomal lncRNA, miRNA and mRNA Expression Profiles and ceRNA Network Construction in Endometriosis

“…Circular RNA (circRNA) is an endogenous ncRNA with a closed circular structure, which can regulate linear RNA transcription, downstream gene expression and protein production [8]. Some researchers have shown that mRNA, miRNA, lncRNA and circRNA can interact as competitive endogenous RNA to form ceRNA regulatory networks [9,10]. LncRNA and circRNA can act as ceRNA molecules and have the function of miRNA sponge [11].…”

Section: Introductionmentioning

confidence: 99%

Whole Transcriptome Analysis on Sepsis Caused by Pyogenic Liver Abscess

Zhao¹,

Liu²,

Han³

et al. 2021

Preprint

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Background Pyogenic liver abscess (PLA) is a common clinical disease throughout the world with remaining high mortality and morbidity. This study sought to investigate key genes and non-coding RNAs (ncRNAs) associated with the pathogenesis and treatment of sepsis caused by PLA (SLA). Methods We constructed the expression profiles of RNAs by RNA sequencing in the peripheral blood of healthy subjects, SLA and seven day therapy of SLA patients. Then the sequencing data was analysed through integrated bioinformatics strategy. Results 4549 mRNAs, 9808 lncRNAs, 467 circRNAs and 292 miRNAs were differentially expressed (DE) between SLA and control samples. 3199 mRNAs, 6018 lncRNAs, 161 circRNAs and 132 miRNAs were differentially expressed between SLA after 7 days therapy and SLA control subjects. Moreover, DE mRNAs of the former two comparisons were both related to immunity and T cell-mediated immune response; DE lncRNAs were both associated with B cell receptor signaling pathway and Osteoclast differentiation; DE circRNAs were both related to Chagas disease and B cell receptor signaling pathway; DE miRNAs exerts its specific role in the process of SLA by MAPK signaling pathway and Estrogen signaling pathway compared to DE lncRNAs, circRNAs and mRNAs. Finally, lncRNA/circRNA–miRNA–mRNA network was constructed to explore the internal regulating relationships of these ncRNAs and mRNAs. Conclusions Our results may provide a new perspective on differential RNA expression involved in the pathogenesis and treatment of SLA and accelerate the development of novel treatments for this disease.

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“…On the one hand, exosomes derived from tumor cells use lncRNA to change normal stromal cells. 16 On the other hand, exosomes from highly malignant tumor cells can regulate the physiological state and function of low malignant tumor cells through lncRNA, so that tumor cells have strong viability. 17 For example, lncRNA HOTAIR is highly expressed in glioma cells, and the highly expressed HOTAIR can be loaded into the exosomes secreted by glioma cells and transferred to endothelial cells, resulting in the increase of angiogenic factor VEGFA in vascular endothelial cells, resulting in enhanced angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

<p>Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686</p>

Chai¹,

Wu²,

Liang³

et al. 2020

IJN

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Objective Glioma is one of the most common central nervous system malignant tumors, accounting for 45%–60% of adult intracranial tumors. However, the clinical treatment of glioma is limited. It is of great significance to seek new therapeutic methods for glioma via gene therapy. Materials and Methods Microarray is used to identify the lncRNAs that are differentially expressed in glioma. The expression of long non-coding RNA (lncRNA) ROR1-AS1 and miR-4686 was detected by qRT-PCR. Exosomes were isolated from the supernatant of normal and cancerous cells, and TEM was used for exosomes identification. MTT assay, wound healing assay, transwell assay, and colony formation assay were used to detect the exo-ROR1-AS1 function on proliferation, migration, and invasion in glioma cells. Luciferase assay and RIP assay were used to identify the relationship between lncRNA ROR1-AS1 and miR-4686. The effect of exo-ROR1-AS1 on tumorigenesis of glioma was confirmed by the xenograft nude mice model. Results ROR1-AS1 was up-regulated in glioma tissues, and the high expression of ROR1-AS1 indicated a poor prognosis in glioma patients. Interestingly, ROR1-AS1 was packaged into exosomes and derived from tumor cells. Functional analysis showed exo-ROR1-AS1 promoted the progression of glioma cell lines SHG44 and U251. Furthermore, ROR1-AS1 acted as a sponge of miR-4686 and inhibited its expression. Functionally, forced expression of miR-4686 removed the promoted effects of lncRNA ROR1-AS1 on glioma development. In vivo tumorigenesis experiments showed that exo-ROR1-AS1 promoted glioma development via miR-4686 axis. Conclusion Our study suggested tumor cells derived exo-ROR1-AS1 promoted glioma progression by inhibiting miR-4686, which might be a potential therapeutic target for glioma clinical treatment.

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Analysis of Exosomal lncRNA, miRNA and mRNA Expression Profiles and ceRNA Network Construction in Endometriosis

Cited by 49 publications

References 49 publications

Construction and topological analysis of an endometriosis-related exosomal circRNA-miRNA-mRNA regulatory network

Construction and topological analysis of an endometriosis-related exosomal circRNA-miRNA-mRNA regulatory network

Whole Transcriptome Analysis on Sepsis Caused by Pyogenic Liver Abscess

<p>Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686</p>

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