Analysis of Essential Oils ofArtemisia absinthiumL. from Lithuania by CC, GC(RI), GC-MS and¹³C NMR

Abstract

Different techniques have been utilized to determine the composition of Artemisia absinthum (wormwood) essential oil. The oil was fractionated on a silica gel column and each fraction analyzed by GC(RI), GC-MS and 13 C NMR. This allowed the identification, for the first time in A. absinthium, of two diterpenes, 9-geranyl-p-cymene and 9-geranyl-α-terpinene, and two homoditerpenes, 9-(15,16-dihydro-15-methylene)-geranyl-p-cymene and 9-(15,16-dihydro-15-methylene)-geranyl-α-terpinene. Chemical variability of A. absinthium essential oils from plants collected in the surroundings of Vilnius city over several years (1999-2007) was also shown. Chemical composition was determined by GC and GC-MS. Thujones (cis+trans, 10.2-36.3%) and trans-sabinyl acetate (9.8-39.2%) were the two predominant constituents of almost all the investigated oils (13 out of 15 samples). The third major compound was myrcene (5.1-9.2%, in four samples), β-pinene (5.4-10.4%, in 5), linalool (4.7% in one), trans-sabinol (6.4%, in one) and 1,8-cineole (5.2-7.1%, in two). In one oil, the prevailing components were thujones (cis+trans, 11.2%), trans-sabinene hydrate (11.0%) and trans-sabinyl acetate (8.8%), while another sample was characterized by a large quantity of trans-sabinyl acetate (55.2%) and the absence of thujones.

Chemical characterization by GC-MS and in vitro activity against Candida albicans of volatile fractions prepared from Artemisia dracunculus, Artemisia abrotanum, Artemisia absinthium and Artemisia vulgaris

“…Similarly, an oil from Northwestern Italy had 1,8-cineole (34.7%), bisabolol oxide (18.4%) and ascaridole (16.0%) as main compounds [ 14 ]. The dominant components in the oil from the Crimea were 1,8-cineole and camphor [ 15 ]. A German A. abrotanum showed 1,8-cineol as main oil compound [ 16 ].…”

“…Cited literature [ 5 , 15 ] described an A. absinthium oil rich in thujone and in the aerial parts of A.vulgaris an oil with high proportions of 1,8-cineole, sabinene, thujone, and caryophyllene oxide.…”

Protective effects ofArtemisia arborescensessential oil on oestroprogestative treatment induced hepatotoxicity

“…Second, the components of Artemisia appear to be able to counteract the oestroprogestative administration induced production of aggressive oxidants. They can also impair the mechanisms by which these oxidants damage the key molecules within the tissues [ 27 ]. The antioxidant properties of Artemisia were attributed to flavonoids, β-Caryophyllene, α-thujone, Boronyl acetate, and phospholipids, which are all known to be efficient radical scavengers [ 28 ].…”

Semiochemical attractants for the beech leaf‐mining weevil,Orchestes fagi

“…pamphylica, davisii, and alanyense (Alan et al, 2011), Artemisia absinthium L. from Tajikistan (Sharopov et al, 2012), and Cydonia oblonga Miller (Gonenc et al, 2012). Both compounds 1 and 2a have been found together in the same plant, for instance, in A. absinthium (Judzentiene et al, 2009), Sideritis dichotoma Huter (Kirimer et al, 2004), and Helichrysum species (Bohlmann et al, 1978). It is plausible that compounds 1 and 2a are generated through the mevalonate pathway via geranylpyrophosphate (Mayo et al, 2012).…”