Analysis of ergosterol for the detection of mold in soils by automated on-fiber derivatization headspace extraction–SPME-GC/MS

Parkinson, Don-Roger; Warren, Jamie; Pawliszyn, Janusz

doi:10.1016/j.aca.2009.12.027

Cited by 25 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When determining organic pollutants in soil by a headspace SPME, derivatization may be used to convert analytes to a more volatile, stable and less polar compounds, improve selectivity, extraction effectiveness and decrease a matrix effect [78]. There are two main options for derivatization when analyzing soil by SPME: in-matrix [79][80][81][82] and on-fiber [83,84]. Most methods involving derivatization are similar to classic sample preparation approaches and, before SPME, they require extraction to an organic solvent [83], an aqueous solution of acid [79,81] or a sub-critical water [80] containing derivatizing agent.…”

Section: Derivatizationmentioning

confidence: 99%

See 1 more Smart Citation

Perspectives and challenges of on-site quantification of organic pollutants in soils using solid-phase microextraction

Kenessov

Kozieł

Bakaikina

et al. 2016

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

This study explores the current state-of-the-art progress toward on-site quantification of organic pollutants in soils with solid-phase microextraction (SPME). In spite of many available methods, only few publications report on-site analyses of soil samples by SPME. To date, the only application of SPME for the on-site quantification of organic pollutants in soil has been devoted to trichloroethylene. The problem of matrix effects limiting quantification by external standard calibration is discussed. Efficiencies of available approaches for decreasing and controlling matrix effects are evaluated and compared. SPME from a soil sample headspace with internal standard calibration was identified as one of the promising approaches to achieve fast, simple, precise, and accurate on-site quantification of a wide range of organic pollutants in soil. Cold-fiber SPME has a significant development potential, because it is capable of providing lowest detection limits together with a minimum matrix effect. Perspectives for future development of the field are outlined.

show abstract

Section: Derivatizationmentioning

confidence: 99%

“…This approach significantly broadens the range of soil pollutants that may be determined by SPME. Derivatization-based methods are available for organometallic compounds [79,80], chemical warfare agents [81], phenols [82] and ergosterol [83].…”

Section: Derivatizationmentioning

confidence: 99%

Perspectives and challenges of on-site quantification of organic pollutants in soils using solid-phase microextraction

Kenessov

Kozieł

Bakaikina

et al. 2016

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

show abstract

“…Isotopic labeled analytes that are thought to react and chromatograph similarly to those analytes being studied have often been employed for monitoring. 28 If derivatization reactions are to ascribe a detection tag to the analytes for signal enhancement, then it is important to maintain the optimal wavelength used and to assess if there are any solvent wavelength shifts (matrix effects) associated with different sample matrices. 103 For HPLC, a method to analyze zoledronic acid in urine and blood samples by derivatization of the bisphosphonate with trimethylsilyl diazomethane (care required!)…”

Section: Quantitation Aspects For Derivatizationmentioning

confidence: 99%

Analytical Derivatization Techniques ☆

Parkinson¹

2014

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

View full text Add to dashboard Cite

For the last 40 years or so, analytical chemists have borrowed well-known reaction techniques from organic and organometallic fields to perform derivatization reactions so as to enhance detection of the elusive analytes they seek. The derivatization mantra has been that, for the technique to be worthwhile, it should be simple to perform, fast (usually less than 15 min), selective, and that the derivatizing reagent should react quantitatively with the analyte and only give one final product. So where are we now? Some derivatization reactions are indeed fast and some are not; some are very selective and some are more universal. However, with a certain care and careful control of optimized conditions, quantitative enhancement outcomes and increased separative power and detection can be very worthwhile. The number of derivatization reagents commercially available itself suggests that there must be some merit to derivatization, that overcomes the associated extra costs: in time (to incorporate the extra reaction step), and in the expense of acquiring, handling, and storing derivatizing reagents. This chapter attempts to show that with proper consideration, it has been feasible to push a chosen analytical technique to give increased selective detection and signal enhancement by derivatization. In many cases, this has made possible the detection of trace components from difficult matrices in a number of scenarios, including on-site analysis, in quality control/continuous analysis methods, or through some remote-sensing platforms. Further, the incorporation of derivatization in an extraction method can be simple and friendly to automated throughput for multiple sample analysis.The fundamental idea of derivatization is that the basic chemical or physical structure of the analytical moiety is changed through a suitable reaction. For example, by labeling compounds for a specific detection purpose, or by changing a functional group to enhance certain chromatographic characteristics, better detection and separation (respectively) can be achieved via the ensuing instrumental techniques. The type of derivatization is highly dependent upon the analytical method and upon the nature of the compound analyzed. There are a variety of good review articles, technical reports, 1 text chapters, 2 and full texts 3-6 that address in detail many specific applications used in analytical derivatization. There are several extraction methods available that are compatible with derivatization. For many analyses, the choice of the best extraction technique is not clear, as many techniques can extract analytes equally well, based on simplicity, applicability, solvent use, expense, detection limit, precision, and time taken to perform the operation. 7 Derivatization techniques have been incorporated into many sample preparation and extractions schemes, to include, but not limited to, total sample headspace, single-drop microextraction (SDME), 8 solid-phase microextraction (SPME), needle trap device (NTD), purge and trap, solid-phase extraction (SPE)...

show abstract

“…It is primarily found as a membrane constituent and is rapidly dispersed to the matrix during cell decomposition. As it is encountered in many types of mould the analysis of ergosterol content has been utilized to determine the extent of mould growth, soil fungal biomass (Parkinson et al, 2010), agriculture quality (Ruzicka et al, 2000), soil nature (Gong et al, 2001;Zhao et al, 2005, de RidderDuine et al, 2006, food deterioration (Kadakal et al, 2005;Abramson and Smith, 2005), mould growth in and on buildings (Görs et al, 2007) and airborne particulates (Lau et al, 2006;Cheng et al, 2008). It has to the best of our knowledge as yet not been applied in biomolecular archaeology, though its absence have been used as an argument for the absence of fungi lipids (as depositional contamination) in archaeological ceramic (Kimpe et al, 2001).…”

Section: Sterolsmentioning

confidence: 99%

Ergosterol (5, 7, 22-ergostatrien-3β-ol) as a potential biomarker for alcohol fermentation in lipid residues from prehistoric pottery

Isaksson

Karlsson

Eriksson

2010

Journal of Archaeological Science

View full text Add to dashboard Cite

Analysis of ergosterol for the detection of mold in soils by automated on-fiber derivatization headspace extraction–SPME-GC/MS

Cited by 25 publications

References 15 publications

Perspectives and challenges of on-site quantification of organic pollutants in soils using solid-phase microextraction

Perspectives and challenges of on-site quantification of organic pollutants in soils using solid-phase microextraction

Analytical Derivatization Techniques ☆

Ergosterol (5, 7, 22-ergostatrien-3β-ol) as a potential biomarker for alcohol fermentation in lipid residues from prehistoric pottery

Contact Info

Product

Resources

About