Analysis of epididymal sperm from Korean native bull (Hanwoo) aged at 8 and 15 months before freezing and after thawing

Spermatozoa motility, viability, acrosome integrity, mitochondrial membrane potential and plasma membrane integrity in 0.25 mL and 0.5 mL straw after frozen-thawing in Hanwoo bull

“…Spermatozoa motility was examined as previously described (Yang et al, 2015;Kang et al, 2016), with slight modifications. In brief, frozen semen was immersed in water at 37.5℃ for 40 s and mixed in a 1.5 mL tube.…”

Comparison of Spermatozoa Recovery Methods on Cauda Epididymal Sperm of Hanwoo Bulls

“…Semen freezing was also performed by Kang et al, (2016) procedure with slight modification. Sperm concentration was adjusted to 40 × 10 6 cells/mL with semen freezing media and preserved at 4°C for 4 hours.…”

“…Motile spermatozoa will swim out to fluid from tubules of cauda epididymis and fluid with spermatozoa will be recovered. For mincing method in bull (Kang et al, 2016), feline (Vernocchi et al, 2014), and stallion (Neuhauser et al, 2013), isolated cauda epididymis was minced finely and fluid with spermatozoa was immediately recovered. And lastly for flushing method, syringe with semen recovery fluid was connected to the vas deferens of the epididiymis of boar (Rath and Niemann, 1997), bull (Papa et al, 2015), ibex red deer (Santiago-Moreno et al, 2009), and rabbit (Brackett et al, 1978).…”