Analysis of epidermal-type transglutaminase (TGase 3) expression in mouse tissues and cell lines

Immunological Detection of Proteolytically Activated Epidermal-type Transglutaminase (TGase 3) Using Cleavage-site-specific Antibody

“…2A). 8) However, probably due to instability, the amount of 30-kDa fragment was much lower than that of the 47-kDa fragment. In order to enrich these molecules in the extract, DEAE anion-exchange chromatography was performed.…”

Characterisation of Urogenital Ridge Gene Expression in the Human Embryonal Carcinoma Cell Line NT2/D1

“…The detection of these transcripts in the testis and NT2/D1 cells suggests that the initial sexual dimorphic expression pattern observed in the ovary is not maintained. Indeed, expression of Tgm3 has been detected in the adult mouse testis [Hitomi et al, 2001] and expression of Irx3 mRNA was reported to be low during embryonic testis development [Jorgensen and Gao, 2005]. No information on the testicular expression of TPH2 or SPRR2D in humans has been reported.…”

Dermatitis herpetiformis: close to unravelling a disease

“…Recent immunohistochemical and immunfluorescence studies with TGe-specific affinity-purified polyclonal and monoclonal antibodies [1,42] showed that TGe is expressed mostly in the upper spinous and granular layers of the epidermis. Immunoblot studies detected only the zymogen form of TGe in the epidermis and keratinocyte culture [42,43]. TGe-specific rabbit polyclonal antibodies failed to bind to the jejunal villous structures by immunofluorescence, whilst TGe mRNA was present in the small bowel when studied by RT PCR [1,43].…”

“…Immunoblot studies detected only the zymogen form of TGe in the epidermis and keratinocyte culture [42,43]. TGe-specific rabbit polyclonal antibodies failed to bind to the jejunal villous structures by immunofluorescence, whilst TGe mRNA was present in the small bowel when studied by RT PCR [1,43]. The amount of the protein and its distribution in the gut is unknown.…”