Analysis of Eosinophilic Round Bodies Formed After Injection of Enamel Matrix Derivative Into the Backs of Rats

Abstract

A high concentration of EMD induces ERBs that consist of a 40-kDa protein which includes a constituent part of amelogenin. The ERBs (or remaining EMD) might promote mesenchymal cell differentiation into hard tissue-forming cells around the EMD injection site.

The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells

“…We synthesized SP based on the sequence, WYQNMIR, by traditional solid-phase peptide synthesis in conjunction with the “tea-bag” methodology using Boc/benzyl-based chemistry, as previously described [11]. SP was prepared at the concentrations of 0, 1, 10, 100 and 1000 ng/mL in culture medium.…”

“…In our previous study, we found that EMD administered underneath the skin on the backs of rats induced cartilage-like tissue formation and eosinophilic round bodies (ERBs) [11]. Analysis of the active sites for ERB binding in EMD-associated proteins was conducted using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.…”

“…Analysis of the active sites for ERB binding in EMD-associated proteins was conducted using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. This database analysis identified a seven amino acid sequence (WYQNMIR) in amelogenin that corresponds to a portion of the amelogenin exon 5 [11]. Based on this sequence, a synthetic oligopeptide (SP) was constructed.…”

Hard Tissue Formation by Human Periodontal Ligament Fibroblast Cells Treated with an Emdogain^|^reg;-Derived Oligopeptide in vitro

“…Therefore, to best utilize the clinical data obtained regarding the regenerative effects and postoperative stability of EMD, EMD-derived synthetic peptides should be developed. Traditional EMD (Emdogain®) was subcutaneously injected into the backs of rats to form eosinophilic corpuscles and chondroid tissue 13) demonstrated that the fragments contained the amino acid sequence, WYQNMIR. Database analysis demonstrated that the sequence corresponded to a swine amelogenin II precursor.…”

“…The optimal concentration of the novel 13) EMD-derived synthetic peptide remains unknown. Here, we used human periodontal ligament fibroblasts (HPdLF) as a model of mesenchymal stem cells to determine the optimal concentration of the peptide required to stimulate their proliferation, adhesion and migration.…”