Analysis of Enterocoliticin, a Phage Tail-like Bacteriocin

Strauch, Eckhard; Kaspar, Heike; Schaudinn, Christoph; Damasko, C.; Konietzny, Antje; Dersch, Petra; Skurnik, Mikael; Appel, Bernd

doi:10.1007/0-306-48416-1_48

Cited by 15 publications

(9 citation statements)

References 4 publications

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“…Our original goal in this work was to identify the OC ligase involved in the Y. enterocolitica O:3 LPS biosynthesis. We hypothesized that an OC‐ligase mutant would not express OC; thus mutants lacking OC would be resistant and would survive selection with enterocoliticin, a bacteriocin that uses OC as its receptor (Strauch et al ., 2003). Using this strategy, we screened a Cat‐Mu transposon library constructed in strain YeO3‐R1 (see Supporting information for details) and isolated several enterocoliticin‐resistant OC‐lacking mutants.…”

Section: Resultsmentioning

confidence: 99%

Identification of three oligo‐/polysaccharide‐specific ligases in Yersinia enterocolitica

Pinta

Batzilla

et al. 2011

Molecular Microbiology

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Section: Resultsmentioning

confidence: 99%

Identification of three oligo‐/polysaccharide‐specific ligases in Yersinia enterocolitica

Pinta

Batzilla

et al. 2011

Molecular Microbiology

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“…(43) and consists of phage tail-like particles that are specific for the OC hexasaccharide (42). Enterocoliticin (10 4 U/ml), kindly provided by E. Strauch (Robert Koch Institute, Berlin, Germany), was used in the same way as described above for R1-37.…”

Section: Methodsmentioning

confidence: 99%

Role of YadA, Ail, and Lipopolysaccharide in Serum Resistance ofYersinia enterocoliticaSerotype O:3

2005

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Complement attack is a host strategy leading to elimination of pathogens. Yersinia enterocolitica expresses several potential complement resistance factors: the outer membrane proteins YadA and Ail as well as lipopolysaccharide (LPS). To study the contribution of these factors to the survival of Y. enterocolitica serotype O:3 in nonimmune human serum, we constructed 23 mutant strains of Y. enterocolitica O:3 expressing different combinations of YadA, Ail, LPS O antigen, and LPS outer core. Survival of bacteria was analyzed in normal serum (with functional classical, lectin, and alternative complement activation pathways) and EGTA-Mg-treated serum (only alternative pathway functional). Kinetic killing tests revealed that the most potent single-serum resistance factor needed for long-term survival was YadA; Ail was also indispensable, but it provided shortterm survival and delayed the bacterial killing. On the contrary, the LPS O antigen and outer core, when in combination with YadA, Ail, or both, had a minor and often negative effect on serum resistance. Bacteria in the exponential phase of growth were more resistant to serum killing than stationary-phase bacteria. After exposing bacteria to EGTA-Mg-treated serum, O antigen could prevent deposition of covalently bound C3b on bacteria at 3 min of incubation, even as a single factor. At later time points (15 and 30 min) it had to be accompanied by YadA, Ail, and outer core. In normal serum, the bacteria were less resistant to C3b deposition. However, no direct correlation between the C3 deposition pattern and bacterial resistance was observed.

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“…Upon contact with susceptible Yersinia strains the phage tails contract and kill the bacterial cell by forming pores in the cell wall leading to a rapid loss of ions (Strauch et al, 2001a). The cell wall receptor of enterocoliticin was identified as the outer core hexasaccharide of the LPS present in certain serotypes of Y. enterocolitica including serotype O:3 (Skurnik et al, 1995(Skurnik et al, , 1999Strauch et al, 2003). The efficacy of enterocoliticin as an antimicrobial agent against infections with pathogenic Y. enterocolitica O:3 strains was assessed recently (Damasko et al, 2005).…”

Section: Phage Productsmentioning

confidence: 99%