“…Aphids were collected after 10 days by brushing them carefully from the leaves into liquid nitrogen before storage at (808C. Aphids were also reared on artificial diets during 10 days before storage at (808C (Febvay et al 1995). The diet contains 10 vitamins (amino benzoic acid, ascorbic acid, biotine, calcium chloride, choline chloride, folic acid, myo-inositol, nicotinic acid, pyroxidine and thiamine), 22 amino acids (alanine, b-alanine, arginine, asparagine, aspartic acid, cystein, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, ornithine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine) …”