Analysis of endotoxin effects on the intact pulmonary circulation

Lambermont, Bernard; Kolh, Philippe; Detry, Olivier; Gérard, Paul; Marcelle, R; D’Orio, Vincenzo

doi:10.1016/s0008-6363(98)00213-2

Cited by 32 publications

(28 citation statements)

References 16 publications

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“…This model of endotoxinic shock has been previously validated and is reproducible (18). They underwent, from T60 onwards, a zero-balance continuous veno-venous hemofiltration at a rate of 45 ml/kg/h.…”

Section: Experimental Protocolmentioning

confidence: 99%

Confirmation of High Cytokine Clearance by Hemofiltration with a Cellulose Triacetate Membrane with Large Pores: An in vivo Study

et al. 2006

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Abstract:Objective: To confirm in vivo the hypothesis that hemofiltration with a large pore membrane can achieve significant cytokine clearance. Method: We used a well-known animal model of endotoxinic shock (0.5 mg/kg of lipopolysaccharide from Escherichia Coli over a period of 30 mins). Six pigs were hemofiltrated for 3 hours with a large pore membrane (78 Å pore, 80 kDa cut off) (Sureflux FH 70, Nipro, Osaka, Japan). The ultrafiltration rate was 45 ml/kg/min. Samples were taken from arterial, venous line and in the ultrafiltrate at T120 and T240. We measured concentrations of interleukin 6, interleukin 10 and albumin. Results: At T120 and T240, the IL-6 clearances were 22 ± 7 and 15 ± 3 ml/min, respectively. The IL-6 sieving coefficients were 0.97 and 0.7 at T120 and T240, respectively. At T120 and T240, the IL-10 clearances were 14 ± 4 and 10 ± 7 ml/min, respectively The sieving coefficients were 0.63 and 0.45 at T120 and T240, respectively. The concentrations of IL-6 and IL-10 were the same at T0 and T240. At T60 and T240, the plasmatic albumin concentrations were 24 ± 4 g/L and 23 ± 4 g/L, respectively (p = 0.13). Conclusions: In this animal model of endotoxinic shock, we confirm the high cytokine clearance observed when hemofiltration is applied to a large pore membrane. The loss of albumin seems negligible. The impact of such clearances on hemodynamic stability and survival remains to be proved.

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Section: Experimental Protocolmentioning

confidence: 99%

Confirmation of High Cytokine Clearance by Hemofiltration with a Cellulose Triacetate Membrane with Large Pores: An in vivo Study

et al. 2006

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“…Further, the inductance leads to positive phase angles in the impedance spectrum [14]. The values of R 1 , R 2 , C, and L were estimated by a method previously described [15].…”

Section: Arterial Propertiesmentioning

confidence: 99%

Increased aortic compliance maintains left ventricular performance at lower energetic cost

Kolh

D’Orio

Lambermont

et al. 2000

European Journal of Cardio-Thoracic Surgery

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Objective: The aim of this study was to investigate left ventricular contractility and energetic cost of cardiac ejection under conditions of acute increase in aortic compliance. Methods: In six anaesthetized pigs, ascending aortic compliance was increased by adding a volume chamber in parallel to the ascending aorta. Systemic vascular parameters, including characteristic impedance, peripheral resistance, total vascular compliance, and inertance, were estimated with a four-element windkessel model. Arterial elastance was derived from these parameters. Left ventricular systolic function was assessed by end-systolic pressure±volume relationship (end-systolic elastance), and stroke work. Pressure±volume area was used as a measure of myocardial oxygen consumption. Heart rate remained constant during the experimentation. Results: Adding the aortic volume chamber signi®cantly increased vascular compliance from 0.95^0.08 to 1.17^0.06 ml/ mmHg (P , 0:01), while inductance, characteristic impedance, peripheral resistance, and arterial elastance remained statistically at basal values, respectively 0.0020^0.0003 mmHg.s 2 /ml, 0.105^0.009 mmHg.s/ml, 1.27^0.12 mmHg.s/ml, and 2.43^0.21 mmHg/ml. During the same interval, stroke work and pressure±volume area decreased respectively from 2700^242 to 2256^75 mmHg.ml (P , 0:01), and from 3806^427 to 3179^167 mmHg.ml (P , 0:01). Stroke work and pressure±volume area decreased at matched end-diastolic volumes. In contrast, end-systolic elastance, ejection fraction, and stroke volume remained statistically unchanged, respectively at 2.29^0.14 mmHg/ ml, 48.1^2.1 %, and 32.4^1.7 ml. Conclusions: These data suggest that, when facing an increased aortic compliance, the left ventricle displays unchanged contractility, but the energetic cost of cardiac ejection is signi®cantly decreased. These data may be of clinical importance when choosing an arti®cial prosthesis for ascending aortic replacement. q

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“…Pulmonary vasonctriction caused by the release of predominantly vasoconstrictive mediators, is an important contributor to the increase in pulmonary vascular resistance in patients with acute pulmonary embolism, hyperacute lung rejection or during the early phase of septic shock (Collins et al, 2001;Lambermont et al, 1999;Smulders, 2000). In septic shock, right heart failure can lead to death (Kimchi et al, 1984) and, in pulmonary embolism, most patients who succumb do so within minutes to few hours after onset of symptoms.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we studied the effects of U-46619 on pulmonary hemodynamics after administration of BM-573 using a four-element windkessel model (Grant & Paradowski, 1987;Lambermont et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Effects of U-46619 on Pulmonary Hemodynamics Before and After Administration of BM-573, a Novel Thromboxane A2 Inhibitor

Lambermont¹,

Kolh

Dogné³

et al. 2003

Archives of Physiology and Biochemistry

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We studied the effects on pulmonary hemodynamics of U-46619, a thromboxane A 2 (TXA 2 ) agonist, before and after administration of a novel TXA 2 receptor antagonist and synthase inhibitor (BM-573). Six anesthetized pigs (Ago group) received 6 consecutive injections of U-46619 at 30-min interval and were compared with six anesthetized pigs (Anta group) which received an increasing dosage regimen of BM-573 10 min before each U-46619 injection. Consecutive changes in pulmonary hemodynamics, including characteristic resistance, vascular compliance, and peripheral vascular resistance, were continuously assessed during the experimental protocol using a four-element Windkessel model. At 2 mg/kg, BM-573 completely blocked pulmonary hypertensive effects of U-46619 but pulmonary vascular compliance still decreased. This residual effect can probably be explained by a persistent increase in the tonus of the pulmonary vascular wall smooth muscles sufficient to decrease vascular compliance but not vessel lumen diameter. Such molecule could be a promising therapeutic approach in TXA 2 mediated pulmonary hypertension as it is the case in pulmonary embolism, hyperacute lung rejection and endotoxinic shock.

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Analysis of endotoxin effects on the intact pulmonary circulation

Abstract: Endotoxemia leads to pulmonary hypertension, which is a result of constriction of proximal pulmonary arteries during the early phase, whereas the late phase is characterised by a decline in distal pulmonary vasculature compliance.

Cited by 32 publications

References 16 publications

Confirmation of High Cytokine Clearance by Hemofiltration with a Cellulose Triacetate Membrane with Large Pores: An in vivo Study

Confirmation of High Cytokine Clearance by Hemofiltration with a Cellulose Triacetate Membrane with Large Pores: An in vivo Study

Increased aortic compliance maintains left ventricular performance at lower energetic cost

Effects of U-46619 on Pulmonary Hemodynamics Before and After Administration of BM-573, a Novel Thromboxane A2 Inhibitor

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