Analysis of endosperm development of allotriploid × diploid/tetraploid crosses in Lilium

Zhou, Shujun; Li, Kehu; Zhou, Guohui

doi:10.1007/s10681-011-0609-5

Cited by 30 publications

(30 citation statements)

References 31 publications

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“…Taken together, these data suggest that the chromosomal compositions of FDR gametes are more balanced than those of IMR gametes, resulting in FDR gametes being more viable and having a higher transmission rate than IMR gametes because of being suitable for selection as cultivars in nature. These findings are in accordance with Zhou et al 2008 studies 36. Indeed, since Lim et al (2001) discovered the IMR mechanism in LA interspecific hybrids 17, Barba-Gonzales (2005a) found that some F 1 OA hybrids produced 2n gametes via an IMR mechanism 20.…”

Section: Discussionsupporting

confidence: 85%

Production of Polyploids and Unreduced Gametes in Lilium auratum × L. henryi Hybrid

Chung¹,

Chung²,

Ramanna³

et al. 2013

Int. J. Biol. Sci.

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Intergenomic F1 hybrids between L. auratum x L. henryi and their BC1 progeny were investigated through genomic in situ hybridization technique (GISH) to determine their potential value in lily breeding. We confirmed that F1 intergenomic hybrids possessed a set of chromosomes (x=12) from both parents and that flowers of the F1 auratum × henryi hybrid showed an intermediate morphological phenotype. Pollen size, viability and germination ability were measured through microscopic observations. F1 intergenomic hybrids produced a relevant frequency of 2n-gametes, which were successfully used to perform crosses with Oriental hybrids, resulting in the triploid Oriental Auratum Henryi (OAuH) hybrid. Twenty BC1 plants were generated by crossing between four different Oriental hybrid cultivars and F1 AuH hybrids using an in vitro embryo rescue technique, after which the genome constitution and chromosome composition were analyzed by GISH. All plants were triploid, showing 12 from female parents (diploid Oriental hybrid) and 24 from male parents (diploid F1 AuH hybrid). Overall, 16 out of 20 BC1 progeny possessed recombinant chromosomes with 1-5 crossover sites per plant. Cytological analysis of 20 BC1 plants by GISH verified that the occurrence of 2n pollen formation in all F1 AuH hybrids was derived from the FDR (first division restitution) mechanism, in which the genome composition of all BC1 plants possess 12 Oriental + 12 L. auratum + 12 L. henryi chromosomes. Allotriploids derived from the AuH hybrid were used as female for crossing with the diploid Oriental hybrid cultivar 'Sorbonne' and considerable numbers of plants (0-6.5 plants per ovary) were only obtained when female OAuH (BC1) triploids were used. Taken together, the results of this study indicate that production and analysis of F1 AuH hybrids and their progeny through sexual polyploidization can be useful for efficient creation of important horticultural traits.

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Section: Discussionsupporting

confidence: 85%

Production of Polyploids and Unreduced Gametes in Lilium auratum × L. henryi Hybrid

Chung¹,

Chung²,

Ramanna³

et al. 2013

Int. J. Biol. Sci.

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“…Most conventional methodology is from 2x × 4x and 4x × 2x hybridization (Kesara 2003;Aleza et al 2010Aleza et al , 2012Moreno et al 2010;Zhou et al 2012;Ji et al 2013). Since 1996, the Plant Protection and Biotechnology Center of the Instituto Valenciano de Investigaciones Agrarias (IVIA, Moncada, Spain) has developed an extensive citrus triploid breeding program based on interploid sexual hybridizations.…”

Section: Artificial Hybridizationmentioning

confidence: 99%

Breeding triploid plants: a review

Wang¹,

Cheng²,

Zhi³

et al. 2016

Czech J. Genet. Plant Breed.

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Wang X., Cheng Z.-M., Zhi S., Xu F. (2016): Breeding triploid plants: a review. Czech J. Genet. Plant Breed., 52: 41-54.Triploid plants have larger organs, greater biomass, and strong stress resistance by preserving relatively larger amounts of photosynthetic energy. The undesirable spread of non-native invasive crop and horticultural plants into natural areas can also be reduced or eliminated by the use of triploids, because they tend to be sterile and seedless. Triploid plants have great economic value and have been useful for developing new agronomic, horticultural, and forestry plant varieties. Because of rapid advances in DNA sequencing technology, triploids may become a focus of genomic research in the future, and will create unprecedented opportunities for discovering and monitoring genomic and transcriptomic changes in unbalanced genomes. One of the new trends in genomics research is to create synthetic triploid plants as materials for the study of first genomic responses that occur immediately after triploid formation. Here, we summarize recent progress in the use of triploid plants, approaches for obtaining triploid plants, including natural selection, artificial hybridization, and endosperm regeneration, the obstacles to obtain triploids, and possible ways to overcome these difficulties. This summary of the scientific progress on triploid plants will promote understanding of how they can be generated and assist plant breeders to design new strategies for triploid breeding.

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“…The GISH has direct applications on the understanding of the genome evolution of polyploid hybrids, partial allopolyploids and recombinant inbred lines, as well as in detecting the amount of introgressed chromatin during the production of new lineages [11][12][13]. Therefore, the GISH has efficiently contributed for the analysis on the karyotypic stability of plant materials, indicating the best genotypes, and helping the assisted selection in different phases of crop improvement [9,14].…”

Section: Introductionmentioning

confidence: 99%