Analysis of enantiomers of sibutramine and its metabolites in rat plasma by liquid chromatography–mass spectrometry using a chiral stationary-phase column

Bae, Kyoungjin; Noh, Kyeumhan; Jang, Kiyoung; Kim, Sohee; Yong, Chul Soon; Choi, Han‐Gon; Kang, Jong Seong; Chen, Jianbo; Ma, Eunsook; Lee, Manhyung; Shin, Beom Soo; Kwon, Kwang‐il; Kang, Wonku

doi:10.1016/j.jpba.2009.04.024

Cited by 18 publications

(12 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantification was performed by multiple reaction-monitoring (MRM) of the protonated precursor ions and the related product ions, using the ratio of the area under the peak for each solution and a weighting factor of 1/y 2 . The analytical data were processed with Analyst software (version 1.4.1, Applied Biosystems) (Bae et al, 2009;Li et al, 2010).…”

Section: Hplc-ms-ms Conditionsmentioning

confidence: 99%

Development of novel sibutramine base-loaded solid dispersion with gelatin and HPMC: Physicochemical characterization and pharmacokinetics in beagle dogs

Lim

Balakrishnan

et al. 2010

International Journal of Pharmaceutics

Self Cite

View full text Add to dashboard Cite

Section: Hplc-ms-ms Conditionsmentioning

confidence: 99%

Development of novel sibutramine base-loaded solid dispersion with gelatin and HPMC: Physicochemical characterization and pharmacokinetics in beagle dogs

Lim

Balakrishnan

et al. 2010

International Journal of Pharmaceutics

Self Cite

View full text Add to dashboard Cite

“…chromatography-mass spectrometry (LC-MS), [8][9][10][11][12][13][14][15][16] gas chromatography-mass spectrometry (GC-MS) 17,18 and ultra-high-pressure liquid chromatography coupled to quadrupole-time-of-flight (UHPLC-QTOF) mass spectrometry. 19 For applications in pharmaceutical products, there are methods that make use of spectrophotometry [20][21][22] and HPLC.…”

mentioning

confidence: 99%

Development and validation of an analytical method by RP-HPLC for quantification of sibutramine hydrochloride in pharmaceutical capsules

et al. 2011

View full text Add to dashboard Cite

Sibutramine is a monoamine re-uptake inhibitor used for the treatment of obesity. In the present study a rapid, sensitive and economical HPLC method was developed and fully validated for analysis of sibutramine HCl in pharmaceutical capsules. HPLC analyses were carried out by using an isocratic elution mode with a mobile phase constituted by sodium phosphate buffer (pH 2.5) and methanol (30 : 70, v/v), flow rate at 1.0 mL min À1, column temperature at 40 C, UV detection wavelength at 225 nm and 20 mL of injection volume. The validation parameters were in accordance with FDA and ICH specifications, showing accuracy, precision, selectivity, robustness and linearity from 4.5 to 19.5 mg L À1 of sibutramine HCl. The limits of detection and quantification were 0.666 and 2.018 mg L À1 , respectively. The validated method is suitable for quality control applications and its advantages over the already existing methods are simplicity and reduced analysis time.

show abstract

“…The authors separated all of the compounds using an a1-acid glycoprotein chiral stationary phase (AGP). Bae et al reported an LC-MS method for determination of ketorolac enantiomers on a chiral AGP column by a gradient elution in 20 min, the matrix effect was found to be negligible and good performances of the method were demonstrated with a positive ESI interface and under multiple reaction monitoring (MRM) conditions (50). Another frequently used reversed-phase CSP is the cellobiohydrolase-based (CBH) chiral column.…”

Section: Protein-based Chiral Stationary Phasesmentioning

confidence: 99%

Review: Recent Application of Chiral Liquid Chromatography-Tandem Mass Spectrometric Methods for Enantiomeric Pharmaceutical and Biomedical Determinations

Nie¹,

Liu²,

Yang³

et al. 2013

Journal of Chromatographic Science

View full text Add to dashboard Cite

The technology of analyzing the pharmacological and toxicological properties of chiral drugs has become more commonly utilized since the knowledge of the biological actions of enantiomers has been gradually acquired during the last few decades. This work reviews the applications of chiral liquid chromatography coupled to tandem mass spectrometry (LC-MS) for the analysis of chiral pharmaceuticals since 2005. The enantioselective determinations in pharmaceutical and biomedical fields with LC-MS are classified based on three mobile phase modes, including normal phase, reversed phase and polar organic solvent, in terms of their compatibility with various ionization sources and specific applications.

show abstract

Analysis of enantiomers of sibutramine and its metabolites in rat plasma by liquid chromatography–mass spectrometry using a chiral stationary-phase column

Cited by 18 publications

References 9 publications

Development of novel sibutramine base-loaded solid dispersion with gelatin and HPMC: Physicochemical characterization and pharmacokinetics in beagle dogs

Development of novel sibutramine base-loaded solid dispersion with gelatin and HPMC: Physicochemical characterization and pharmacokinetics in beagle dogs

Development and validation of an analytical method by RP-HPLC for quantification of sibutramine hydrochloride in pharmaceutical capsules

Review: Recent Application of Chiral Liquid Chromatography-Tandem Mass Spectrometric Methods for Enantiomeric Pharmaceutical and Biomedical Determinations

Contact Info

Product

Resources

About