Analysis of elicitor-inducible transcripts encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in potato

Stermer, Bruce A.; Edwards, Lesley; Edington, Brent V.; Dixon, Richard A.

doi:10.1016/0885-5765(91)90024-c

Cited by 23 publications

(27 citation statements)

References 16 publications

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“…Some of these metabolic changes have been correlated with changes in HMGR activity (Stermer and Bostock, 1987;Chapell et al, 1991) or differential expression of specific HMGR genes (Stermer et al, 1991;Yang et al, 1991;Choi et al, 1992;Cramer et al, 1993). We showed that the Arabidopsis HMGP gene promoter is not induced in wounded leaves of transgenic tobacco plants containing construct (-857/+64)HMGP-GUS.…”

Section: Discussionmentioning

confidence: 99%

Expression of the Arabidopsis HMG2 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, is restricted to meristematic and floral tissues.

et al. 1995

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M o n t s e r r a t Enjuto,' Victoria Lumbreras, Carles Marín, a n d A l b e r t B o r o n a t 2 Unitat de Bioquímica i Biologia Molecular A, Departament de Bioquímica i Fisiologia, Facultat de Química, Universitat de Barcelona, 08028 Barcelona, SpainThe synthesis of mevalonate, which is considered the first rate-limiting step in isoprenoid biosynthesis, is catalyzed by the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR; EC 1.1.1.34). In Arabidopsis, HMGR is encoded by two differentially expressed genes (HMG1 and HMGP). The transcriptional activity of the HMGP gene was studied after fusing different regions of its 5' flanking region to the P-glucuronidase (GUS) reporter gene and transforming the resulting constructs into tobacco plants. The spatial and temporal expression directed by the HMGP promoter in the transgenic plants is consistent with the expression pattern previously established by RNA analysis using an HMGBspecific probe. HMGP expression is restricted to meristematic (root tip and shoot apex) and floral (secretory zone of the stigma, mature pollen grains, gynoecium vascular tissue, and fertilized ovules) tissues. Deletion analysis of the HMGP 5'flanking region was conducted in transgenic plants and transfected protoplasts. The region containing nucleotides -857 to +64 of the HMGP gene was sufficient to confer high levels of expression in both floral and meristematic tissues, although deletion to nucleotide -503 resulted in almost complete loss of expression. Sequences contained within the 5' transcribed, untranslated region are also important for gene expression. The biological significance of the restricted pattern of expression of HMGP is also discussed.

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Section: Discussionmentioning

confidence: 99%

Expression of the Arabidopsis HMG2 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, is restricted to meristematic and floral tissues.

et al. 1995

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“…During the course of our studies, two articles appeared concerning wound and arachidonic acid induction of HMGR mRNAs in potato tuber (Stermer et al, 1991;Yang et al, 1991). In the study by Yang et al (1991), DNA probes from a tomato HMGR genomic clone were used to monitor HMGR expression in tuber discs.…”

Section: Differential Expression Of Potato Hmgr Genes In Response To mentioning

confidence: 99%

“…However, in our study, gene-specific probes corresponding to the 3' untranslated regions of three tomato HMGR genes showed expression patterns in potato discs treated with arachidonic acid (data not shown) identical to those observed using potato gene-specific probes ( Figure 5). Stermer et al (1991) used polymerase chain reaction amplification of total RNA from arachidonic acid-induced tuber discs with oligonucleotide primers complementary to highly conserved regions of HMGR genes to obtain a series of HMGR clones. The sequences of two of these clones (Potl7 and Pot30) are nearly identical in the regions of overlap to hmg2 and hmg3 reported here.…”

Section: Differential Expression Of Potato Hmgr Genes In Response To mentioning

confidence: 99%

“…lnhibitor studies indicate that HMGR activities and phytoalexin synthesis are transcriptionally controlled (Oba et al, 1985). Recently, Yang et al (1991) and Stermer et al (1991) demonstrated wound and elicitor induction of different HMGR mRNAs in potato, but they did not address whether the suppression of the wound-induced steroid pathway after elicitor treatment was also evident at the level of expression of different HMGR genes. In this article, we report the isolation and characterization of three cDNAs corresponding to different genes for HMGR in potato and demonstrate their differential expression after treatment of tuber tissue with the fungal elicitor arachidonic acid (Bostock et al, 1981(Bostock et al, , 1982 or after inoculation with the fungal pathogen Phyrophthora infestans.…”

Section: Introductionmentioning

confidence: 99%

“…In higher plants, mevalonic acid serves as the basic precursor for all cellular isoprenoids. Results from molecular studies in severa1 plant systems suggest highly coordinated but complex regulatory mechanisms for the expression of different HMGR isoforms (Caelles et al, 1989;Learned and Fink, 1989;Narita and Gruissem, 1989;Bach et al, 1991aBach et al, , 1991bChoi et al, 1991;Stermer et al, 1991;Yang et al, 1991;Chye et al, 1992). SquaIene synthetase and sesquiterpene cyclase catalyze, respectively, the first steps in the branches leading from farnesyl diphosphate to sterols (steroid glycoalkaloids) and sesquiterpenoid phytoalexins.…”

Section: Introductionmentioning

confidence: 99%

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Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor arachidonic acid.

1992

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lnduction of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is essential for the biosynthesis of sesquiterpenoid phytoalexins and steroid derivatives in Solanaceous plants following stresses imposed by wounding and pathogen infection. To better understand this complex step in stress-responsive isoprenoid synthesis, we isolated three classes of cDNAs encoding HMGR (hmgl, hmg2, and hmg3) from a potato tuber library using a probe derived from an Arabidopsis HMGR cDNA. The potato cDNAs had extensive homology in portions of the protein coding regions but had low homology in the 3' untranslated regions. RNA gel blot analyses using gene-specific probes showed that hmgl was strongly induced in tuber tissue by wounding, but the wound induction was strongly suppressed by treatment of the tissue with the fungal elicitor arachidonic acid or by inoculation with an incompatible or compatible race of the fungal pathogen Phytophthora infestans. The hmg2 and hmg3 mRNAs also accumulated in response to wounding, but in contrast to hmgl, these mRNAs were strongly enhanced by arachidonic acid or inoculation. lnoculation with a compatible race of R infestans resulted in similar patterns in HMGR gene expression of hmg2 and hmg3 except that the magnitude and rate of the changes in mRNA levels were reduced relative to the incompatible interaction. The differential regulation of members of the HMGR gene family may explain in part the previously reported changes in HMGR enzyme activities following wounding and elicitor treatment. The suppression of hmgl and the enhancement of hmg2 and hmg3 transcript levels following elicitor treatment or inoculation with the incompatible race parallel the suppiession in steroid and stimulation of sesquiterpenoid accumulations observed in earlier investigations. The results are discussed in relation to the hypothesis that there are discrete organizational channels for sterol and sesquiterpene biosynthesis in potato and other Solanaceous species.

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Biochemical and Molecular Tools for the Production of Useful Terpene Products from Pepper (Capsicum Annuum)

Lozoya‐Gloria

1999

Advances in Experimental Medicine and Biology

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Analysis of elicitor-inducible transcripts encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in potato

Cited by 23 publications

References 16 publications

Expression of the Arabidopsis HMG2 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, is restricted to meristematic and floral tissues.

Expression of the Arabidopsis HMG2 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, is restricted to meristematic and floral tissues.

Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor arachidonic acid.

Biochemical and Molecular Tools for the Production of Useful Terpene Products from Pepper (Capsicum Annuum)

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