Analysis of Electron Spin Resonance Spectra of Alkyl Spin Labels in Excised Guinea Pig Dorsal Skin, Its Stratum Corneum, Delipidized Skin and Stratum Corneum Model Lipid Liposomes.

Electron Paramagnetic Resonance Investigation of Stratum Corneum Lipid Structure

“…Electron paramagnetic resonance (EPR) is useful for elucidating structural aspects of stratum corneum (SC) [1][2][3][4][5]. Non-invasive spectroscopic characterization of the outermost layer of the SC is an important subject in dermatology and cosmetology.…”

“…The ordering of the lipid bilayer can be studied by X-ray analysis only for in vitro SC specimens or model lipid membranes composed of water. Structural information can be obtained by analysis of aliphatic spin probes incorporated into the lamellar lipids using EPR [1][2][3][4][5]. Therefore, EPR can potentially detect the skin-lipid structures as well as their dynamics.…”

“…The EPR spin probe method has been utilized to characterize the skin-lipid structures [1,2], as well as fluidity [3]. The physicochemical properties of intercellular lipids of SC were investigated as a function of various surfactants [4], and water content [5].…”

Stratum Corneum Lipid Structure Investigated by EPR Spin‐Probe Method: Application of Terpenes

“…The effect of chemical enhancers on SC lipids can be found by the analyses of aliphatic spin probes incorporated into intercellular lamella lipids using electron paramagnetic resonance (EPR). EPR in conjunction with a spin probe method non-destructively measures the ordering of the lipid bilayer of SC [1][2][3][4][5][6]. Moreover, limited investigations have focused on the mechanisms of the permeation of chemicals.…”

“…On the other hand, EPR is a sensitive and nondestructive technique to measure the probe moieties in the lipid bilayers at ambient temperature [1][2][3][4][5][6]. The EPR probe method can provide an insight into the SC lipid organization as well as its dynamics.…”