Analysis of eicosapentaenoic and docosahexaenoic acid geometrical isomers formed during fish oil deodorization

Fournier, Véronique; Juanéda, Pierre; Destaillats, Frédéric; Dionisi, Fabiola; Lambelet, Pierre; Sébédio, Jean‐Louis; Berdeaux, Olivier

doi:10.1016/j.chroma.2006.06.089

Cited by 56 publications

(58 citation statements)

References 30 publications

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“…These FA isomers would contribute to the FT-NIR signal and influence the quantitation based on the FT-NIR model. It was previously shown that heating fish oil produces trans isomers of the highly unsaturated FA, particularly EPA and DHA, that could only be resolved using very long highly polar capillary columns [34][35][36]. Even though every effort is made to prevent and isolate these products during the production of n-3 PUFA, small amounts were confirmed using the 100 m column (Fig.…”

Section: Ft-nir Quantification Of Fatty Acidsmentioning

confidence: 96%

“…Polypropylene glycol type columns, such as the FAMEWAX column from Restek, or the SUPELCOWAX 10 column from Supelco Inc., have the additional benefit of greater stability of the stationary phase, since the phase is cross-bonded to the fused silica column [30,33,[37][38][39]. On the other hand, if trans containing isomers are present that need to be resolved from the all cis natural unsaturated FA isomers, the highly polar cyanopropyl polysiloxane 100 m fused silica capillary column are recommended [31][32][33][34][35]40]. In the case of the fish oil and the ethyl ester mixtures, using the 100 m column resolved several trans-18:1, 18:2, and 18:3 isomers as well as many trans containing PUFA, which were originally present in the fish oil or were produced in any of the fractionation and/or purification steps in the industrial processes of producing n-3 PUFA concentrates.…”

Section: Comparison Of the Two Gc Columnsmentioning

confidence: 99%

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Rapid quantitation of fish oil fatty acids and their ethyl esters by FT‐NIR models

Azizian¹,

Kramer

Ehler³

et al. 2010

Euro J Lipid Sci & Tech

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Consumption of fish oil and dietary supplements containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) has steadily increased because of their reported health benefits. A rapid procedure based on Fourier Transform Near Infrared Spectroscopy (FT-NIR) models was developed for analysis of fish oil and their ethyl ester derivatives to replace the time consuming GC method. Inclusion of fish oil or ethyl esters containing varied concentrations of OA, EPA, and DHA into the FT-NIR classification models made possible their classification and quantification. Accurate GC analysis is essential in developing reliable quantitative models since FT-NIR is matrix dependent. Development of FT-NIR models based on 30 m PEG capillary GC column results, as recommended by the official GC method for analysis of marine oils, proved problematic, since these columns did not resolve many geometric isomers compared to 100 m highly polar cyanopropyl polysiloxane columns. Depending on the content of geometric isomers in fish oils and ethyl esters, the levels of long-chain n-3 PUFA would be overestimated if the model used were based on the results from a 30 m column. The FT-NIR method was found to be applicable to all fish oil and ethyl ester samples, except when fatty acids were outside the range examined, or contaminants were present. The FT-NIR method was applicable to analysis of in-plant intermediates provided contaminants were absent, or identified so they could be incorporated into the model. The FT-NIR method was suitable to evaluate the shelf life of n-3 PUFA concentrates.

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Section: Ft-nir Quantification Of Fatty Acidsmentioning

confidence: 96%

Section: Comparison Of the Two Gc Columnsmentioning

confidence: 99%

Rapid quantitation of fish oil fatty acids and their ethyl esters by FT‐NIR models

Azizian¹,

Kramer

Ehler³

et al. 2010

Euro J Lipid Sci & Tech

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show abstract

“…52 -54) focus on trans octadecenoic (18:1) FA, but applications on polyunsaturated trans FA are also described. 55 Ag-TLC and Ag-HPLC were found to be fully equivalent for this purpose. 56 The quantitative Ag-TLC/ densitometry of isopropyl esters was successfully used to estimate the seasonal variation of trans FA in butter fats.…”

Section: ·2 Separation Of Cis/trans Isomersmentioning

confidence: 88%

Advances in Silver Ion Chromatography for the Analysis of Fatty Acids and Triacylglycerols—2001 to 2011

Momchilova

Nikolova‐Damyanova

2012

ANAL. SCI.

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“…In milk fat, TFAs are produced by anaerobic fermentation of PUFAs in the rumen of lactating cows (Destaillats et al, 2007;Fournier et al, 2006). This fermentation process is called biohydrogenation, and it results in TFAs that can be further metabolised in the mammary gland.…”

Section: Studies In Animalsmentioning

confidence: 99%

Trans Fatty Acids and Human Health

Filip¹,

Vidrih²

2012

The Cardiovascular System - Physiology, Diagnostics and Clinical Implications

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Analysis of eicosapentaenoic and docosahexaenoic acid geometrical isomers formed during fish oil deodorization

Cited by 56 publications

References 30 publications

Rapid quantitation of fish oil fatty acids and their ethyl esters by FT‐NIR models

Rapid quantitation of fish oil fatty acids and their ethyl esters by FT‐NIR models

Advances in Silver Ion Chromatography for the Analysis of Fatty Acids and Triacylglycerols—2001 to 2011

Trans Fatty Acids and Human Health

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