Analysis of Earphone Swab Mitochondrial Dna as an Alternative Material for Identification Examination

Yudianto, Ahmad; Sispitasri, Yeti Eka; Margaret, Nola

doi:10.20473/fmi.v52i3.5446

Cited by 4 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, Yudianto et al [10] performed a sample with earphones that were used for a period of 3 days. After this period of usage, the earphones were left at room temperature for a period of 5 days, 10 days, and a maximum of 20 days.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Mitochondrial DNA on Earwax as an Alternative Material for Present and Future Forensic Examination

Felipe

2018

GJO

View full text Add to dashboard Cite

Introduction: Nowadays molecular forensics is a field of science evolving since the1980s, known as DNA fingerprinting. Specimens widely used in DNA examine for identification are blood spots/bloods, semen spots, vaginal swabs, buccal swabs and bones. In addition to these samples, the last objects often used by the perpetrators/victims can be hearing assistive devices or/and hearing aids(headsets/earphones). In its use, earphones are attached to the outer ear skin; therefore, the earwax is suspected to stick to the device. Cerumen have been investigated for the last decades finding an important way to detect specific variations in the mitochondrial DNA (mtDNA) of this type of corporal fluid. The present study discusses relevant information about the literature and how this method is the future for new findings in forensic sciences.Discussion: Literature showed that Mitochondrial DNA has better durability and relatively higher number of copies than those of nuclear DNA. This leads to greater possibility of success in amplification, given the higher number of mitochondrial DNA copies and the fact that mitochondrial DNA is a single locus that allows recombination. It is controversial the use of earwax as a possible DNA identification tool because of the environmental factors of exposure duration. Controversially, literature shows that this type of fluid has a great opportunity in terms of cell replication and DNA identification. Conclusions:The mt DNA contained in earwax samples potentials to be a new identification sample for the forensic science. Present studies provide promising evidence for the use of this fluid in different types of identification procedures.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of Mitochondrial DNA on Earwax as an Alternative Material for Present and Future Forensic Examination

Felipe

2018

GJO

View full text Add to dashboard Cite

show abstract

“…A study that conducted by Hanssen et al showed that degree of degradation in samples of sperm and blood spots had an effect on the interpretation of the results of PCR (Polymerase Chain Reaction) and Sequencing (6). Another study by Yudianto et al by using a swab examination on earphones detected using MtDNA Hypervariable Region II showed a decrease from day 1 to day 20 (7). There are so many items that have the potential to contain human DNA, such as the miswak, which is often used to clean teeth in addition to using a toothbrush.…”

Section: Introductionmentioning

confidence: 99%

Amplification of vWA, FGA, and TH01 loci of DNA Samples Isolated From Ring Stored at Room Temperature

Furqoni,

Ayu Megasari,

Yudianto

et al. 2023

MJMHS

Self Cite

View full text Add to dashboard Cite

Introduction: Acts of criminal behaviour that occur have various modes and motives. Also, criminals always try to hide or eliminate evidence at the crime scene. In most cases, police or forensic experts often find DNA on items at the crime scene. One of these items is a ring, which is an item that humans often wear. Methods: This study used 24 samples of rings that had been worn for 8 hours and were incubated at room temperature. All these 24 samples then were distinguished into 4 groups, in which each group was consisting of 6 samples and incubated for 0, 1, 3, and 7-days. DNA identification was then carried out using UV spectrometer for DNA quantification and DNAzol method for DNA extraction. Results: The mean result of DNA quantification on day 0 (control) was 1020,833 ± 0.28903 ng/μL, day 1 was 546 ± 0.093569 ng/μL, day 3 was 1066.333 ± 0.117372 ng/μL, and day 7 was 1054.083 ± 0.070733 ng/μL. PCR process used STR primers with loci vWA, FGA, and TH01 and visualization used the silver nitrate method. Conclusion: The final results showed that all samples could be amplified using 3 STR loci, namely vWA, FGA, and TH01.

show abstract

“…Earphones might be used by offenders or suspects with mobile phones or MP3s attached to the outer ear, and thus, the outer ear earwax possibly sticks to these devices. Previous reports have shown that in the absence of commonly encountered biological traces at the crime scene, used earphones and other hearing devices containing traces of earwax can be effectively used in forensic casework [3][4][5] by employing mitochondrial DNA (mtDNA) sequencing of hypervariable regions 1 and 2 of human earwax mtDNA. Despite its forensic significance, published work regarding the use of human earwax for the purpose of autosomal STR genotyping is very limited [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Short Tandem Repeat (STR) Profiling of Earwax DNA Obtained from Healthy Volunteers

Amer,

Alotaibi,

Shahid

et al. 2023

CIMB

View full text Add to dashboard Cite

The present study aimed to establish human earwax as a potential source of DNA evidence that could be effectively used in human identification. Sixty earwax samples were obtained from 15 healthy male and female Saudi volunteers living in Riyadh, Saudi Arabia. Four consecutive earwax swab samples were obtained from each volunteer and stored for 1, 15, 30 and 60 days. Earwax samples were stored at room temperature (20–22 °C). Reference oral swab was also taken from each volunteer. DNA was extracted by QIAamp DNA Mini kit and quantified by real-time polymerase chain reaction (RT-PCR) on 7500 Thermal Cycler. Autosomal STR loci were amplified using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit (Thermo Fisher Scientific, Carlsbad, CA, USA). Amplified fragments were size separated and analyzed on a 3500 Genetic Analyzer. Complete autosomal STR profiles were obtained from the earwax swabs of all the volunteers stored up to 30 days after the collection. Some STR profiles were partially obtained 60 days after the earwax collection. Allelic drop-out, allelic drop-in, and stutters were seen in earwax samples analyzed 60 days after the collection. The results have shown that human earwax can be a potential source of DNA evidence for human identification up to 30 days after the earwax collection. It is recommended to quickly analyze earwax samples or store them at room temperature or at −10 °C after their recovery from the crime scene.

show abstract

Analysis of Earphone Swab Mitochondrial Dna as an Alternative Material for Identification Examination

Abstract: ABSTRAKIdentifikasi termasuk sidik jari, properti, kesehatan, gigi, serologi

Cited by 4 publications

References 8 publications

Analysis of Mitochondrial DNA on Earwax as an Alternative Material for Present and Future Forensic Examination

Analysis of Mitochondrial DNA on Earwax as an Alternative Material for Present and Future Forensic Examination

Amplification of vWA, FGA, and TH01 loci of DNA Samples Isolated From Ring Stored at Room Temperature

Short Tandem Repeat (STR) Profiling of Earwax DNA Obtained from Healthy Volunteers

Contact Info

Product

Resources

About