Analysis of DNA synthesis in cell cultures of the adult newt cardiac myocyte

Tate, John M.; Oberpriller, John O.

doi:10.1016/0040-8166(89)90048-7

Cited by 12 publications

(2 citation statements)

References 26 publications

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“…Unlike skeletal muscle cells that proliferate as undifferentiated myoblasts, proliferating cardiomyoblasts express a full panel of sarcomeric proteins and contract in the fetal heart. When these cardiac myocytes divide, the sarcomeres disassemble to allow for cytokinesis and then reassemble in the two contractile daughter cells similar to newt cardiomyocytes (113). A detailed analysis of cardiac myocyte size and number in adult human hearts concluded that while the mass of the heart may change through adolescence and in different disease conditions, the number of cardiomyocytes does not (131), although this has recently been challenged (68,73).…”

Section: B Origins Of the Dogma That The Mammalian Heart Is A Nonregmentioning

confidence: 98%

“…Cardiomyocytes isolated from newts reenter the cell cycle when stimulated with mitogens, with half these cardiac myocytes becoming multinucleated while the other half undergo division. To divide, newt cardiomyocytes need to partially dissembled their sarcomeric structures and dedifferentiate (phenotypically regress from a differentiated cardiac myocyte into more primitive cell state) (113). Mature zebrafish or newts have substantial cardiac regenerative capacity, being able to restore myocardial structure even after removal of a large portion of the heart apex (40,47,54).…”

Section: A Cardiac Myocyte Proliferation In Lower Vertebratesmentioning

confidence: 99%

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Cardiac Regeneration and Stem Cells

Zhang

Mignone

MacLellan

2015

Physiological Reviews

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After decades of believing the heart loses the ability to regenerate soon after birth, numerous studies are now reporting that the adult heart may indeed be capable of regeneration, although the magnitude of new cardiac myocyte formation varies greatly. While this debate has energized the field of cardiac regeneration and led to a dramatic increase in our understanding of cardiac growth and repair, it has left much confusion in the field as to the prospects of regenerating the heart. Studies applying modern techniques of genetic lineage tracing and carbon-14 dating have begun to establish limits on the amount of endogenous regeneration after cardiac injury, but the underlying cellular mechanisms of this regeneration remained unclear. These same studies have also revealed an astonishing capacity for cardiac repair early in life that is largely lost with adult differentiation and maturation. Regardless, this renewed focus on cardiac regeneration as a therapeutic goal holds great promise as a novel strategy to address the leading cause of death in the developed world.

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Section: B Origins Of the Dogma That The Mammalian Heart Is A Nonregmentioning

confidence: 98%

Section: A Cardiac Myocyte Proliferation In Lower Vertebratesmentioning

confidence: 99%

Cardiac Regeneration and Stem Cells

Zhang

Mignone

MacLellan

2015

Physiological Reviews

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Comparison of mitosis in binucleated and mononucleated newt cardiac myocytes

Matz

Oberpriller

1998

Anat. Rec.

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Background: The cultured adult newt ventricular myocyte has been shown to undergo mitosis and cytokinesis in a fully differentiated state. Insight into its proliferation and cellular changes during the repair process involves obtaining a better understanding of the nuclear pattern (mononucle-ated, binucleated, or multinucleated) resulting from mitotic events. Mitosis is easily observable in cultured newt cardiac myocytes using phase-contrast microscopy. Methods: From days 8-19 in culture, the process of mitosis in mononucle-ated and binucleated newt ventricular myocytes was recorded and timed by using time-lapse video microscopy. Cultured cardiac myocytes were double-stained for myosin and F-actin by using fluorescein isothiocyanate (FITC)-labeled MF20 and rhodamine phalloidin. Results: Mitotic, mononucleated myocytes produced mononucleated daughter cells in 80% of the cases, whereas 20% were single, binucleated myocytes. In binucleated myocytes, only 32% underwent complete cytokinesis to produce two binucleated daughter cells, whereas 68% resulted in variably nucleated myocytes. Mononucleated and binucleated myocytes undergoing mitosis had similar time intervals for the period from nuclear breakdown (prometaphase) to the start of anaphase (108.7 minutes and 94.5 minutes, respectively), but the period between anaphase and midbody formation was significantly shorter in binucleated than in mononucleated myocytes (43.5 minutes and 69.3 minutes, respectively). The myofibrillae were not as well organized in binucleated myocytes as those observed in mononucleated myocytes. Conclusions: Mitosis in vitro appears to proceed more rapidly in binucle-ated newt cardiac myocytes, which have more poorly organized myofibrillae than mononucleated myocytes. Mitosis of cultured binucleated myocytes commonly results in variably nucleated daughter cells, whereas mononucle-ated myocytes produce predominantly mononucleated daughter cells. Anat.

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Reactivation of cardiomyocyte cell cycle: A potential approach for myocardial regeneration

McMullen

Gaspard

Pasumarthi

2005

Signal Transduction

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Regulation of cardiomyocyte cell cycle appears to be more complex in mammals compared to the lower vertebrates. Cardiomyocytes from the adult newt and zebrafish can proliferate in response to myocardial injury and regenerate the damaged area. In contrast, cardiomyocytes in the mammalian heart cease to proliferate soon after birth. This limits the ability of the mammalian heart to regenerate the damaged myocardium following heart disease. It is believed that increasing the number of myocytes in a diseased heart can decrease scar formation and improve myocardial function. To this end, reactivation of cell cycle in the surviving myocardium may have therapeutic value in the treatment of heart disease. Here we provide a summary of studies describing myocyte cell cycle activity during development and disease, mechanisms of cell cycle exit in the adult heart and genetic modulations affecting cardiomyocyte cell cycle activity. Further, we discuss the potential utility of myocyte cell cycle reactivation in cardiac regeneration as well as improvement of myocardial function.

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Analysis of DNA synthesis in cell cultures of the adult newt cardiac myocyte

Cited by 12 publications

References 26 publications

Cardiac Regeneration and Stem Cells

Cardiac Regeneration and Stem Cells

Comparison of mitosis in binucleated and mononucleated newt cardiac myocytes

Reactivation of cardiomyocyte cell cycle: A potential approach for myocardial regeneration

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