Analysis of DNA adducts of acetaldehyde by liquid chromatography–mass spectrometry

Acetaldehyd [MAK Value Documentation in German language, 2008]

“…Das hauptsächliche DNA-Addukt von Acetaldehyd ist N 2 -Ethylidendesoxyguanosin (Hecht et al 2001 a, b;Inagaki et al 2003;Vaca et al 1995;Wang et al 2000), eine Schiffsche Base. Acetaldehyd reagiert dabei mit der exozyklischen Aminogruppe des Guanins zum entsprechenden relativ instabilen Imin (Fang und Vaca 1995).…”

“…Acetaldehyd führte zur Bildung von DNA-Addukten mit isolierter DNA (Fang und Vaca 1995;Fraenkel-Conrat und Singer 1988;Inagaki et al 2003;Vaca et al 1995;Wang et al 2000; siehe auch Abschnitt 2) und mit Säugerzellen . Die einstündige Inkubation von menschlichen Wangenschleimhautzellen mit Acetaldehyd in Konzentrationen von 0, 10, 30 oder 100 mM führte zu Bildung von 0,7; 1,9; 2,2 und 2,7 N 2 -Ethyldesoxyguanosin-Addukten pro 10 7 Nukleotiden.…”

Functional Preconcentration Tip of Total Volume Injection for ESI/MS Analysis of DNA Adducts

“…Conveniently, MS detection of DNA adducts is more sensitive in base forms than in deoxynucleoside forms. 10 Next, we used CPr-Gua as model DNA adduct bases. Because base forms have higher polarity compared with deoxynucleosides, the recovery ratios were examined with varying ODS amounts.…”

Detection of 1,N2-propano-2′-deoxyguanosine in human urine by stable isotope dilution UHPLC–MS/MS analysis

“…Acetaldehyde (Ace) and crotonaldehyde (Cro) are both mutagenic and carcinogenic aldehyde [3][4][5][6][7]. They are present in tobacco smoke, vehicle exhaust and fuel combustion gases [2,4].…”

“…Meanwhile, ProdG adducts are also major products from the reaction of dG with Cro [1,6]. ProdG adducts have been found in genomic DNA in various tissues of animals and human [3,6,12]. In vivo, ProdG adducts can inhibit DNA synthesis by promoting DNA miscoding through G → T transversion [6,12].…”