Analysis of diphenylarsinic acid in human and environmental samples by HPLC-ICP-MS

Abstract

A simple, rapid and robust analytical method for determining diphenylarsinic acid in human and environmental samples was developed based on a combination of hydrophilic polymer-based gelpermeation high-performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Hair and nail samples were digested with alkali, and liberated diphenylarsinic acid (derivative) was extracted with diethyl ether, redissolved in water and injected for HPLC-ICP-MS analysis. Human urine, groundwater and water extracts from soils were injected for HPLC-ICP-MS directly after filtration. Using the method, diphenylarsinic acid in a solution was quantified in 7 min duration for an analysis with a detection limit of sub-nanograms per milliliter. The method has been applied to groundwater arsenic pollution recently uncovered in Japan.

Fractionation and Speciation of Trace Elements in Soil Solution

“…Although SEC is most commonly used for the separation of relatively large compounds such as polymers and biopolymers, it can be used for the separation of compounds of intermediate molecular weight as well. For example, Shibata et al [39] determined diphenylarsinic acid in urine, groundwater, and soil extract samples by SEC-ICP-MS. The analysis was performed with a sub-parts-per-billion detection limit.…”

Synthesis of phenyl arsenic analytical standards related to contaminated well water in Kamisu, Ibaraki, Japan

“…All products were crystallized from ethanol. 1 13 C NMR data and other properties of the triarylarsines are given in Table 1.…”

“…1 Analysis showed that the arsenic was present almost entirely as diphenylarsinic acid 1 and was thus very unlikely to have a natural origin. The source of this compound to the ground water supplying the well is unknown, although the most likely explanation is that arsenical war gases or their precursors were dumped and buried in the area during, or immediately following, the World war II.…”

“…LC-ICP-MS analysis of diphenylarsinic acid in water samples is conveniently carried out on a column that, although nominally facilitating separation of analytes by size exclusion or gel permeation, relies substantially, in the absence of a buffered eluent, on hydrophobicity. 1 Thus, it was expected that analogues of diphenylarsinic acid in which the non-polar aromatic portion was increased by the addition of alkyl groups might serve as the required internal standards.…”

Transformation of diphenylarsinic acid and related compounds in groundwater: production of thiol-containing arsenicals

“…1) was carried out. Research activities have included the development of analytical techniques, [2] monitoring of the concentrations of DPAA and its transformation products in human urine and groundwater, [3,4] studies of the absorption and metabolism of DPAA by plants, [5,6] studies of the toxicodynamics of DPAA in experimental animals [7,8] and studies aimed at elucidating the mechanism of DPAA's toxicity. [9] During recent measurements using liquid chromatographyinductively coupled plasma-mass spectrometry (LC-ICP-MS) of DPAA and related arsenicals in groundwater samples, we detected unknown arsenic species that appeared to be derived from DPAA.…”