Analysis of differential gene expression by bead-based fiber-optic array in growth-hormone-secreting pituitary adenomas

Abstract

Abstract. Growth-hormone-secreting pituitary adenomas (Ghomas) account for approximately 20% of all pituitary neoplasms. however, the pathogenesis of Ghomas remains to be elucidated. to explore the possible pathogenesis of Ghomas, we used bead-based fiber-optic arrays to examine the gene expression in five GHomas and compared them to three healthy pituitaries. Four differentially expressed genes were chosen randomly for validation by quantitative real-time reverse transcription-polymerase chain reaction. We then performed pathway analysis on the identified differentially expressed genes using the Kyoto Encyclopedia of Genes and Genomes. Array analysis showed significant increases in the expression of 353 genes and 206 expressed sequence tags (ESts) and decreases in 565 genes and 29 ESts. Bioinformatic analysis showed that the genes HIGD1B, HOXB2, ANGPT2, HPGD and BTG2 may play an important role in the tumorigenesis and progression of Ghomas. pathway analysis showed that the wingless-type signaling pathway and extracellular-matrix receptor interactions may play a key role in the tumorigenesis and progression of Ghomas. Our data suggested that there are numerous aberrantly expressed genes and pathways involved in the pathogenesis of GHomas. Bead-based fiber-optic arrays combined with pathway analysis of differentially expressed genes appear to be a valid method for investigating the pathogenesis of tumors.

Microarray data analysis reveals differentially expressed genes in prolactinoma

“…Our results supported the idea that differential expression of extracellular matrix components, integrins and matrix metalloproteinase contributes to the control of pituitary hormone production and cell proliferation during tumorigenesis [25]. Similarly, the role of ECM receptor interactions pathway is revealed in the tumorigenesis and progression of Growthhormone-secreting pituitary adenomas [26]. Protein complex 3 was associated with amino acid metabolism, tryptophan, arginine and proline metabolisms, as well as decomposition of limonene and pinene, and that was also in line with enriched signaling pathways.…”

Inhibition of Notch signaling attenuates pituitary adenoma growth in Nude mice

“…It is downregulated in preneoplastic and neoplastic lesions in various cancers (Farioli-Vecchioli et al 2007, Mao et al 2015, and it intersects with the Notch pathway (Farioli-Vecchioli et al 2014). It is found in the embryonic and adult anterior pituitary (Terra et al 2008), and, using the NCBI database (http://www.ncbi.nlm.nih.gov/Genbank/) to analyze the differentially expressed genes in plurihormonal and GH pituitary adenomas compared with healthy pituitaries, BTG2 was found downregulated (Jiang et al 2010(Jiang et al , 2012. In accordance, our experimental and bioinformatic results support its role as a tumor suppressor in the pituitary and suggest its regulation by Notch.…”