Analysis of differential gene expression during floral bud abortion in radish (Raphanus sativus L.)

Abstract

ABSTRACT. Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) and real-time polymerase chain reaction (real-time PCR). A total of 221 differentially expressed transcript-derived fragments (TDFs) were detected by 256 cDNA-AFLP primer combinations, of which 114 were upregulated and 107 were downregulated in the aborted buds. A total of 54 TDFs were cloned and sequenced. A BLAST search revealed that all TDFs have homologous sequences and 29 of these corresponded to known genes, whose functions were mainly related to metabolism, stimulus response, transcriptional regulation, and transportation. Expressions of 6 TDFs with different functions were further analyzed by real-time PCR yielding expression profiling results consistent with the cDNA-AFLP analysis. Our results indicated that radish FBA is related to abnormalities in various physiological and biochemical plant processes.

Normal and Abortive Buds Transcriptomic Profiling of Broccoli ogu Cytoplasmic Male Sterile Line and Its Maintainer

“…Bud abortion is a very common biological phenomenon in Brassica species. During bud abortion, the buds stop growing, convert from green into yellow progressively from the base to the top of the bud, and eventually wither off before flowering [ 1 , 2 ], which is detrimental for hybrid seeds yield during cross breeding. In recent years, bud abortion has attracted considerable attention in Brassica species cross breeding [ 3 , 4 , 5 ], as it reduces the hybrid seed yield production of many Brassica crops and also affects the efficiency of Brassica crops cross breeding.…”

“…In recent years, bud abortion has attracted considerable attention in Brassica species cross breeding [ 3 , 4 , 5 ], as it reduces the hybrid seed yield production of many Brassica crops and also affects the efficiency of Brassica crops cross breeding. In the past decade, to solve the bud abortion problem, many studies have investigated the factors that cause bud abortion [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ]. However, most of the studies focused on morphology and anatomy, and few studies have reported the factors contributing to Brassica bud abortion at the molecular level.…”