Analysis of developmental gene expression using smFISH andin silicostaging ofC. elegansembryos
Laura Breimann,
Ella Bahry,
Marwan Zouinkhi
et al.
Abstract:Regulation of transcription during embryogenesis is key to development and differentiation. To study transcript expression throughout Caenorhabditis elegans embryogenesis at single-molecule resolution, we developed a high-throughput single-molecule fluorescence in situ hybridization (smFISH) method that relies on computational methods to developmentally stage embryos and quantify individual mRNA molecules in single embryos. We applied our system to sdc-2, a zygotically transcribed gene essential for hermaphrod… Show more
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