Analysis of deep sequencing exosome‐microRNA expression profile derived from CP‐II reveals potential role of gga‐miRNA‐451 in inflammation

Zhao, Yabo; Fu, Yali; Zou, Meiling; Sun, Yingfei; Yin, Xun; Niu, Liangjie; Gong, Yanzhang; Peng, Xiuli

doi:10.1111/jcmm.15244

Cited by 19 publications

(15 citation statements)

References 49 publications

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“…The results showed that some items of biological process were involved in regulating cellular process, single-organism process and metabolic process; some items of cellular component were involved in regulating cell part, intracellular membrane-bounded organelle; and some items of molecular functions were involved in regulating protein binding, organic cyclic compound binding and catalytic activity as reported elsewhere (Zhao et al, 2020).…”

Section: Figure 3 Go Database Function Notessupporting

confidence: 70%

Mycoplasma pathogenicity for humans and animals: an effort of complete genome sequencing and gene family analysis of Mycoplasma synoviae

Yi¹

2021

PAKJAS

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Like humans, chickens can become sick from bacterial infections. Mycoplasma is a very small, slow-growing bacterium. There are multiple types of Mycoplasma that affect different animals, humans, and even plants and insects. In the current study, the whole genome of Mycoplasma synoviae was sequenced through the joint sequencing method of the third-generation PacBio and second-generation Illumina of the high-throughput sequencing platform, and the complete genome map of the Mycoplasma synoviae was successfully obtained. SMRT sequencing and assembly of whole genome: Canu v1.5/ WTDBG v2.2 software was used for preliminary assembly of filtered subreads. Pilon V1.22 software was used to further correct the assembled genome with high-quality second-generation data, and the genome with higher accuracy was finally obtained. Sequencing results showed that the Mycoplasma was composed of a 0.83Mb chromosome with a G+C percentage of 28.42%. Bioinformatics was used to predict the encoding protein genes, and it was found that Mycoplasma synoviae genome encoded a total of 1415 ORF open reading frames, including 1.73% of the repeats, 7 ribosomal RNAs, 34 transport RNAs, 4 non-coding RNAs and 12 pseudogenes. Mycoplasma synoviae genomes also contain 2 CRISPR, 1 gene island and 2 prophages. Using multiple strains of Mycoplasma gallisepticum, Mycoplasma iowae and Mycoplasma anatis as reference genomes, we constructed phylogenetic trees of 12 species of Mycoplasma. The genetic and taxonomic status of Mycoplasma synoviae strains were evaluated. The results showed that the sequencing strains of Mycoplasma synoviae and the model strain Mycoplasma synoviae WVU1853 were closely clustered together, suggesting the genetic relationship was relatively close and the sequencing strains were relatively conservative, and represent that they share the same genetic origin.

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Section: Figure 3 Go Database Function Notessupporting

confidence: 70%

Mycoplasma pathogenicity for humans and animals: an effort of complete genome sequencing and gene family analysis of Mycoplasma synoviae

Yi¹

2021

PAKJAS

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“…Emerging evidence demonstrates P-gp-related exosomal miRNAs can lead to long-term P-gp expression in recipient cells [ 52 ]. For example, exosomal miRNA-451 and miRNA-27a are abundant in recipient cells, which could explain these long-term effects [ 53 ].…”

Section: The Functions Of Exosomal Mirnas In Cancermentioning

confidence: 99%

The role of Exosomal miRNAs in cancer

Zhou

Chen

et al. 2022

J Transl Med

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Exosomal miRNAs have attracted much attention due to their critical role in regulating genes and the altered expression of miRNAs in virtually all cancers affecting humans (Sun et al. in Mol Cancer 17(1):14, 2018). Exosomal miRNAs modulate processes that interfere with cancer immunity and microenvironment, and are significantly involved in tumor growth, invasion, metastasis, angiogenesis and drug resistance. Fully investigating the detailed mechanism of miRNAs in the occurrence and development of various cancers could help not only in the treatment of cancers but also in the prevention of malignant diseases. The current review highlighted recently published advances regarding cancer-derived exosomes, e.g., sorting and delivery mechanisms for RNAs. Exosomal miRNAs that modulate cancer cell-to-cell communication, impacting tumor growth, angiogenesis, metastasis and multiple biological features, were discussed. Finally, the potential role of exosomal miRNAs as diagnostic and prognostic molecular markers was summarized, as well as their usefulness in detecting cancer resistance to therapeutic agents.

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“…All RNA oligonucleotides were designed and synthesized by GenePharm (Shanghai, China) and are shown in Table 2. [53]. Brie y, it was prepared from lung tissue of 15 day SPF chicken embryos.…”

Section: Microrna Target Prediction and Sequencesmentioning

confidence: 99%

Exosomal miR-181a-5p Protects against MG Infection by Targeting PPM1B and Activating the TLR2-Mediated MyD88/NF-κB Pathway

Sun¹,

Zhao²,

Pang³

et al. 2020

Preprint

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BackgroundMycoplasma gallisepticum (MG) is one of the most important pathogens that causes chronic respiratory disease (CRD) in chickens. Exosomes secreted from cells have been well documented to deliver miRNAs to recipient cells to modulate cellular function. The purpose of this current study was to explore the functions of exosomal miR-181a-5p in MG infection and the underlying mechanisms. ResultsHere, we found that miR-181a-5p expression in vivo and in vitro was significantly upregulated after MG infection. Exosomes enriched in MG-infected chicken type-II pneumocytes (CP-II) could selectively load miR-181a-5p and transfer it into recipient DF-1 cells. PPM1B was further identified as the target gene of miR-181a-5p. Overexpression of miR-181a-5p and/or knockdown of PPM1B activated the TLR2-mediated MyD88/NF-κB signaling pathways, whereas inhibition of miR-181a-5p and the overexpression of PPM1B led to the opposite results. In addition, depressing miR-181a-5p significantly reduced the expression of tumor necrosis factors alpha (TNF-α) and interleukin-1β (IL-1β) by MG-induced. Upregulated miR-181a-5p promoted cell proliferation and cell cycle progression and inhibited apoptosis to resist MG infection. Moreover, overexpression of miR-181a-5p significantly depressed pMAG1.2 expression by directly inhibiting PPM1B.ConclusionsTaken together, we conclude that the newly identified primary CP-II cells exosomal miR-181a-5p activates the TLR2-mediated MyD88/NF-κB pathway by directly targeting PPM1B to promote pro-inflammatory cytokines expression for defending against MG infection in recipient DF-1 cells.

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Analysis of deep sequencing exosome‐microRNA expression profile derived from CP‐II reveals potential role of gga‐miRNA‐451 in inflammation

Cited by 19 publications

References 49 publications

Mycoplasma pathogenicity for humans and animals: an effort of complete genome sequencing and gene family analysis of Mycoplasma synoviae

Mycoplasma pathogenicity for humans and animals: an effort of complete genome sequencing and gene family analysis of Mycoplasma synoviae

The role of Exosomal miRNAs in cancer

Exosomal miR-181a-5p Protects against MG Infection by Targeting PPM1B and Activating the TLR2-Mediated MyD88/NF-κB Pathway

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