Analysis of Dabsyl-Cl Derivated Amino Acids by High Performance Liquid Chromatography and Tandem Mass Spectrometry

Abstract

The results showed better identification of the dabsyl-AA and the method is, therefore, more reliable in analyzing amino acid contents in complex food mediums.

Mass spectrometric determination of dabsyl‐chloride derivatised anserine, carnosine and taurine in commercial chicken essences

“…However, neither [dabsyl-Tau + 2H] 2+ (m/z 207) nor [dabsyl 2 -Tau + 2H] 2+ (m/z 351) were found. This result clearly suggested that the dabsyl-Tau was the major product of dabsyl-Cl derivatised taurine, which was similar to that of our previous analysis on amino acids when each dabsyl-Cl derivatised amino acid showed an m/z value 287 greater than that of the corresponding amino acid (Chen et al, 2003). The extra 287 value suggests that only one dabsyl group will react with one amino acid molecule, and so did the same result in this study with taurine.…”

“…Therefore, Soga & Heiger (2000) developed a capillary electrophoresis electrospray ionisation mass spectrometry (CE-ESI-MS) to take advantage the efficient resolution of CE and the high selectivity and sensitivity of MS. HPLC-MS has poor resolution because of the limitation of the mobile phase, but has worked well with some of the derivatised amino acids (van Leuken et al, 1995;Liu et al, 1995;Fujii et al, 1998). We had proved the HPLC-MS/MS to be a sensitive and reliable method in the analysis of dabsyl-Cl derivatised amino acids in a complex medium (Chen et al, 2003). In this study, we are extending the HPLC-MS/MS to the analysis of anserine, carnosine and taurine in chicken essences.…”

“…4a). The transition from m/z 413 to 224 in MS/MS analysis was therefore chosen as the SRM setting for [dabsyl-Tau + H] + , which was same as those for most of the dabsyl-Cl derivatised amino acids in previous article (Chen et al, 2003). The product ion spectrum of [dabsyl-Ans + 2H] 2+ is shown in Fig.…”

“…Although the sensitivity of these analytical approaches may be further improved by photodiode array detection, it is still considered not as selective and accurate as mass spectrometry (MS) when samples are complexes of protein hydrolysates or meat extracts containing proteins, peptides and amino acids (Gartenmann & Kochhar, 1999;Chen et al, 2003). The only analysis thus far concerning free anserine, carnosine and taurine concentrations in commercial chicken and other meat essences had been reported by Wu & Shiau (2002), who derivatised post-column elutes by ninhydrin and then analysed with an amino acid analyser.…”

Melatonin and its potential biological functions in the fruits of sweet cherry

“…An HPLC system, modified from Chen et al. , was consisted of a reverse phase 5 μ m Symmetry (4.6 × 250 mm) column (Waters, Milford, MA, USA) connected with an HP 1100 Agilent HPLC, and equipped with an HP1046A Agilent fluorescence detector (Agilent Technologies, Palo Alto, CA, USA). A mixture of acetonitrile: 50 m m Na 2 HPO 4 /H 3 PO 4 buffer pH 4.5 (15:85) was used as mobile phase.…”