Analysis of cytochrome P450 metabolites of arachidonic acid by stable isotope probe labeling coupled with ultra high-performance liquid chromatography/mass spectrometry

Zhu, Quan-Fei; Hao, Yanhong; Liu, Ming-Zhou; Yue, Jiang; Ni, Jian; Yuan, Bi-Feng

doi:10.1016/j.chroma.2015.07.100

Cited by 58 publications

(38 citation statements)

References 31 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Measurement of specific types of BAs by mass spectrometry (MS) was previously described. 37 Briefly, the combined supernatant was dried under nitrogen gas and reconstituted in 100 μL acetonitrile followed by sequential addition of 20 μL 2-chloro-1methylpyridinium iodide (20 μmol/mL), 40 μL triethylamine (20 μmol/mL) and 40 μL d4-2-dimethylaminoethylamine (20 μmol/ mL). The reaction solution was vortexed at 40°C for 1 h and then evaporated under nitrogen gas.…”

Section: Measurement Of Intracellular Basmentioning

confidence: 99%

Virus-induced accumulation of intracellular bile acids activates the TGR5-β-arrestin-SRC axis to enable innate antiviral immunity

Gao

et al. 2019

Cell Res

Self Cite

View full text Add to dashboard Cite

The mechanisms on metabolic regulation of immune responses are still elusive. We show here that viral infection induces immediate-early NF-κB activation independent of viral nucleic acid-triggered signaling, which triggers a rapid transcriptional induction of bile acid (BA) transporter and rate-limiting biosynthesis enzymes as well as accumulation of intracellular BAs in divergent cell types. The accumulated intracellular BAs activate SRC kinase via the TGR5-GRK-β-arrestin axis, which mediates tyrosine phosphorylation of multiple antiviral signaling components including RIG-I, VISA/MAVS, MITA/STING, TBK1 and IRF3. The tyrosine phosphorylation of these components by SRC conditions for efficient innate antiviral immune response. Consistently, TGR5 deficiency impairs innate antiviral immunity, whereas BAs exhibit potent antiviral activity in wild-type but not TGR5-deficient cells and mice. Our findings reveal an intrinsic and universal role of intracellular BA metabolism in innate antiviral immunity.

show abstract

Section: Measurement Of Intracellular Basmentioning

confidence: 99%

Virus-induced accumulation of intracellular bile acids activates the TGR5-β-arrestin-SRC axis to enable innate antiviral immunity

Gao

et al. 2019

Cell Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…- . LOXs catalyze regio-and stereospecific dioxygenation of PUFAs containing 1,4-cis,cis-pentadiene units into hydroperoxy fatty acids (HPFAs), a type of oxylipin enzymes that use ARA are 11-LOXs in cyanobacteria (4), corals (24), algae (25), cyclooxygenases, and cytochrome P450s (26). However, all of the reported enzymes produce 11R-HETE.…”

mentioning

confidence: 99%

Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus

2018

Journal of Lipid Research

View full text Add to dashboard Cite

“…It has been used for the derivatization of a series of 19 eicosanoids including hydroxy, dihydroxy, and epoxy eicosatetraenoic acids for their quantification in rat tissue and serum samples from diabetic and cancer patients using UPLC–MS/MS. Minimum matrix effect and improved sensitivity (5–138‐fold) were achieved using this technique (Zhu, Hao, et al, ). The same pair of isotopes were also used for profiling carboxylic‐containing metabolites in biological specimens from smokers and non‐smokers.…”

Section: Isotope‐coded Derivatization Reagents For Lc–ms Analysismentioning

confidence: 99%

“…Apart from ICD reagents dedicated to the biomedical field, another piperazine reagent pair, 2 H 0 / 2 H 3 -10-methyl-2-(piperazin- (Zhu, Hao, et al, 2015). The same pair of isotopes were also used for profiling carboxylic-containing metabolites in biological specimens from smokers and non-smokers.…”

Section: Piperazine Reagentsmentioning

confidence: 99%

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

El-Maghrabey

Kishikawa

Kuroda

2020

Biomedical Chromatography

View full text Add to dashboard Cite

Currently, LC–MS has various applications in different areas such as metabolomics, pharmacokinetics, and pathological studies. Yet, matrix effects resulting from co‐existing constituents remain a major problem for LC–MS [or LC–tandem mass spectrometry (LC–MS/MS)]. Moreover, technical problems and instrumental drifts may lead to ion abundance variance. Thus, an internal standard (IS) is required to guarantee the accuracy and precision of the method. Because of their limited number, isotope‐coded derivatization (ICD) has been recently introduced to overcome this problem. For ICD, a stable heavy isotope‐coded moiety is used for labeling the standard or the control sample and the formed products can act as ISs. A light form of the reagent is used for labeling the sample. Then, both are mixed and analyzed by LC–MS(/MS). This strategy permits the identification of different unknown analytes including potential metabolites and disease biomarkers. All these attributes lead to persistent growth in the applications of ICD LC–MS(/MS) in various biomedical branches. In this article we review the ICD methods published in the last eight years for biomedical applications as well as briefly summarize other applications for environmental and food analyses as some of their used ICD reagents were further applied for analyzing biological specimens or have the potential for that.

show abstract

Analysis of cytochrome P450 metabolites of arachidonic acid by stable isotope probe labeling coupled with ultra high-performance liquid chromatography/mass spectrometry

Cited by 58 publications

References 31 publications

Virus-induced accumulation of intracellular bile acids activates the TGR5-β-arrestin-SRC axis to enable innate antiviral immunity

Virus-induced accumulation of intracellular bile acids activates the TGR5-β-arrestin-SRC axis to enable innate antiviral immunity

Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

Contact Info

Product

Resources

About