Analysis of Cyclic Adenosine Monophosphate (cAMP) Separation via RP-HPLC (reversed-phase high-performance liquid chromatography) by the Moment Method and the van Deemter Equation
Abstract:요 약고성능액체크로마토그래피(high-performance liquid chromatography, HPLC)에서 C18(octadecyl silica, ODS) 칼럼에서 고리형 아데노신 일인산(cyclic adenosine monophosphate, cAMP)의 크로마토그램을 얻은 후, 모멘트 분석을 수행하였 다. 일반속도 모델(general rate model, GR model)을 기반으로 first absolute moment와 second central moment를 계산 하였다. 모멘트 분석의 중요한 세 가지 계수인 분자확산계수(molecular diffusivity, D m ), 외부물질전달계수(external mass transfer coefficient, k f ), 입자내부확산계수(intra-particle diffusivity, D e )는 각각 Wilke-Chang 식, Wilson-Geankoplis 식을 이용하고 이론단수(theoretical plate number) 식과 van Dee… Show more
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