Analysis of CoQ10 in rat serum by ultra-performance liquid chromatography mass spectrometry after oral administration

Li, Lie; Pabbisetty, Deepthi; Carvalho, Paulo; Avery, Mitchell A.; Avery, Bonnie A.

doi:10.1016/j.jpba.2007.10.019

Cited by 19 publications

(8 citation statements)

References 20 publications

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“…Various liquid chromatography/tandem mass spectrometry (LC/MS/MS) methods for measurement of CoQ 10 have been described . However, CoQ 10 forms Na + and K + adducts which can be difficult to fragment, thus limiting the sensitivity and utility of these methods.…”

mentioning

confidence: 99%

Coenzyme Q₁₀ quantification in muscle, fibroblasts and cerebrospinal fluid by liquid chromatography/tandem mass spectrometry using a novel deuterated internal standard

Duberley

Hargreaves

Chaiwatanasirikul³

et al. 2013

Rapid Comm Mass Spectrometry

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mentioning

confidence: 99%

Coenzyme Q₁₀ quantification in muscle, fibroblasts and cerebrospinal fluid by liquid chromatography/tandem mass spectrometry using a novel deuterated internal standard

Duberley

Hargreaves

Chaiwatanasirikul³

et al. 2013

Rapid Comm Mass Spectrometry

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“…In previously reported LC-MS-based assays for CoQ10 in biological samples, either CoQ11 (14 ), CoQ9 (15)(16)(17)19 ), or dipropoxy-CoQ10 (18 ) has been used as the IS. A potential source of error for MS assays is ionization suppression that varies during a chromatographic run and between the samples.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial Coenzyme Q10 Determination by Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry

2013

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BACKGROUND Coenzyme Q10 (CoQ10) is an essential part of the mitochondrial respiratory chain. Unlike most other respiratory chain disorders, CoQ10 deficiency is potentially treatable. We aimed to develop and validate an accurate liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the determination of mitochondrial CoQ10 in clinical samples. METHODS We used mitochondria isolated from muscle biopsies of patients (n = 166) suspected to have oxidative phosphorylation deficiency. We also used fibroblast mitochondria from 1 patient with CoQ10 deficiency and 3 healthy individuals. Samples were spiked with nonphysiologic CoQ10-[2H6] internal standard, extracted with 1-propanol and with ethanol and hexane (2 mL/5 mL), and CoQ10 quantified by LC-MS/MS. The method and sample stability were validated. A reference interval was established from the patient data. RESULTS The method had a limit of quantification of 0.5 nmol/L. The assay range was 0.5–1000 nmol/L and the CVs were 7.5%–8.2%. CoQ10 was stable in concentrated mitochondrial suspensions. In isolated mitochondria, the mean ratio of CoQ10 to citrate synthase (CS) activity (CoQ10/CS) was 1.7 nmol/U (95% CI, 1.6–1.7 nmol/U). We suggest a CoQ10/CS reference interval of 1.1–2.8 nmol/U for both sexes and all ages. The CoQ10/CS ratio was 5-fold decreased in fibroblast mitochondria from a patient with known CoQ10 deficiency due to recessive prenyl (decaprenyl) diphosphate synthase, subunit 2 (PDSS2) mutations. CONCLUSIONS Normalization of mitochondrial CoQ10 concentration against citrate synthase activity is likely to reflect most accurately the CoQ10 content available for the respiratory chain. Our assay and the established reference range should facilitate the diagnosis of respiratory chain disorders and treatment of patients with CoQ10 deficiency.

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“…n-Hexane and propanol have been widely used for the extraction of CoQ10 from biological matrixes (Edlund, 1988;Okamoto et al, 1988;Grossi et al, 1992;Yamashita and Yamamoto, 1997;Menke et al, 2000;Tang et al, 2001Tang et al, , 2004Mosca et al, 2002;Lu et al, 2003;Galinier et al, 2004;Hansen et al, 2004;Karpinska et al, 2006;Li et al, 2006Li et al, , 2008Hatanaka et al, 2008;Prosek et al, 2008;Balakrishnan et al, 2009;Paliakov et al, 2009), although extraction with less volatile propanol requires more time-consuming solvent evaporation under an air steam. The efficiency of different mixtures of organic solvents for the extraction of CoQ10 was compared under our experimental conditions.…”

Section: Methods Developmentmentioning

confidence: 99%

“…1997;Menke et al, 2000;Tang et al, 2001Tang et al, , 2004Mosca et Galinier et al, 2004;Miles et al, 2008), and LC coupled with mass spectrometry (MS) (Hansen et al, 2004;Li et al, 2008;Prosek et al, 2008). HPLC-ECD appears to be most commonly used for the simultaneous determination of CoQ10 and CoQ10H2 because of its high selectivity and sensitivity; however, this methodology is complicated, time-consuming and rather used in the clinical laboratory.…”

Section: Introductionmentioning

confidence: 99%

Validated HPLC method for the quantitative determination of CoQ₁₀ in dog plasma and its application to a pharmacokinetic study

Yuan¹,

Liu²,

Xu³

et al. 2010

Biomedical Chromatography

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Coenzyme Q(10) (CoQ(10) ) is a naturally occurring compound located in all membranes throughout the cell. A rapid and sensitive HPLC method was developed to determine the concentration of CoQ(10) in dog plasma using a surrogate matrix. Chromatographic separation was carried out on a Diamonsil C(18) column with the UV detector set at 275 nm. Methanol-2-propanol (40:60, v/v) was used as a mobile phase delivered at a flow rate of 1.0 mL/min. Calibrators were prepared using blank plasma-K(2) HPO(4) buffer (50 mm, pH 8.0)-saline (1:3:6, v/v/v) as surrogate matrix. It was shown that the surrogate matrix had similar properties to dog plasma for CoQ(10) in extraction, freeze-thaw and stability. The assay was linear over the concentration range of 0.10-100 µg/mL. The intra- and inter-day precisions were within 13.3% in terms of relative standard deviation (RSD%) and the accuracy was within ±7.5% in terms of relative error. This simple and reproducible HPLC method with less plasma volume (0.4 mL) and adequate sensitivity was successfully applied to pharmacokinetic studies of CoQ(10) in dogs and an investigation of the effect of CoQ(10) formulation on CoQ(10) baseline levels.

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Analysis of CoQ10 in rat serum by ultra-performance liquid chromatography mass spectrometry after oral administration

Cited by 19 publications

References 20 publications

Coenzyme Q₁₀ quantification in muscle, fibroblasts and cerebrospinal fluid by liquid chromatography/tandem mass spectrometry using a novel deuterated internal standard

Coenzyme Q₁₀ quantification in muscle, fibroblasts and cerebrospinal fluid by liquid chromatography/tandem mass spectrometry using a novel deuterated internal standard

Mitochondrial Coenzyme Q10 Determination by Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry

Validated HPLC method for the quantitative determination of CoQ₁₀ in dog plasma and its application to a pharmacokinetic study

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Analysis of CoQ10 in rat serum by ultra-performance liquid chromatography mass spectrometry after oral administration

Cited by 19 publications

References 20 publications

Coenzyme Q10 quantification in muscle, fibroblasts and cerebrospinal fluid by liquid chromatography/tandem mass spectrometry using a novel deuterated internal standard

Coenzyme Q10 quantification in muscle, fibroblasts and cerebrospinal fluid by liquid chromatography/tandem mass spectrometry using a novel deuterated internal standard

Mitochondrial Coenzyme Q10 Determination by Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry

Validated HPLC method for the quantitative determination of CoQ10 in dog plasma and its application to a pharmacokinetic study

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About

Coenzyme Q₁₀ quantification in muscle, fibroblasts and cerebrospinal fluid by liquid chromatography/tandem mass spectrometry using a novel deuterated internal standard

Coenzyme Q₁₀ quantification in muscle, fibroblasts and cerebrospinal fluid by liquid chromatography/tandem mass spectrometry using a novel deuterated internal standard

Validated HPLC method for the quantitative determination of CoQ₁₀ in dog plasma and its application to a pharmacokinetic study