1999
DOI: 10.1002/(sici)1098-2795(199909)54:1<17::aid-mrd3>3.0.co;2-n
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Analysis of control elements for position-independent expression of human ?-lactalbumin YAC

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Cited by 15 publications
(2 citation statements)
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“…The use of YACs in transgenesis is likely to ensure position-independent, copy-number independent, and optimal levels of expression for transgenes, as long as all the regulatory elements are located within the vector (Giraldo and Montoliu, 2001). YACs have been used to produce transgenic mice, pigs (Yannoutsos et al, 1995), rabbits (Brem et al, 1996;Rouy et al, 1998), and rats (Fujiwara et al, 1997(Fujiwara et al, , 1999. In livestock, the benefits of YACs are mainly focused on xenotransplantation and the efficient production of recombinant proteins (Giraldo and Montoliu, 2001).…”
Section: Microchromosome Transfermentioning
confidence: 99%
“…The use of YACs in transgenesis is likely to ensure position-independent, copy-number independent, and optimal levels of expression for transgenes, as long as all the regulatory elements are located within the vector (Giraldo and Montoliu, 2001). YACs have been used to produce transgenic mice, pigs (Yannoutsos et al, 1995), rabbits (Brem et al, 1996;Rouy et al, 1998), and rats (Fujiwara et al, 1997(Fujiwara et al, , 1999. In livestock, the benefits of YACs are mainly focused on xenotransplantation and the efficient production of recombinant proteins (Giraldo and Montoliu, 2001).…”
Section: Microchromosome Transfermentioning
confidence: 99%
“…So far, Alac from various species were overexpressed only specifically in the lactating mammary gland of transgenic mammals using Alac-encoding gene regulatory sequences or, in one case, the αs1-casein promoter (Fujiwara et al, 1999). To assess if forced expression of this gene during early gestation or involution could affect mammary physiology, potentially highlighting the significance of some of the noncanonical functions attributed to Alac, a goat Alac-encoding minigene was expressed in transgenic mice under the transcriptional regulation of the mouse mammary tumor virus (MMTV) promoter derived from the pMSG expres- ; t1 = unspliced transgene-derived transcript; t2 = spliced transcript; t3 = unexpected shorter transcript; + = RT-PCR with addition of reverse transcriptase; -= PCR on product of reverse transcriptase reaction lacking reverse transcriptase enzyme, as negative control.…”
mentioning
confidence: 99%