Analysis of contamination of endodontic absorbent paper points

Pereira, Érica Rodrigues; Nabeshima, Cleber Keiti; Machado, Manoel Eduardo de Lima

doi:10.1590/s1980-65232011000100013

Cited by 3 publications

(3 citation statements)

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“…Several studies have reported the contamination of some brands of these materials, even in unopened packages [7][8][9]. Similarly, different authors have reported contamination when the packaging is opened or contamination owing to inadequate handling [810], which indicates that there may be several causes for this contamination.…”

Section: Introductionmentioning

confidence: 99%

Contamination of paper points used by students during preclinical and clinical endodontic procedures

Angarita

2020

BDS

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Objective: To determine the presence of facultative anaerobic bacteria in the paper points used by students and to perform a pilot test to determine whether sterilization of these materials influences their absorption capacity. Material and Methods: This study consists of two phases. The first is a descriptive phase where a representative sample of paper points (n = 72) was collected from the students and information about the points was voluntarily contributed. The points were placed in saline solution after they were collected, and mechanically shaken for 60 s. Then, 300 IU of the solution was seeded on blood agar in duplicates and incubated for 5 days under anaerobic conditions. The second phase was experimental, during which five paper points of each of the existing sizes were sterilized (Numbers: 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 70, and 80), and their capacity to absorb water was compared with that of the control or non-sterilized points. Results: The study determined that 22% (n=16) of the points were primarily contaminated by gram-positive bacilli, followed by gram-positive cocci, among which Staphylococcus epidermidis was identified. The presence of contamination was not significantly associated with the conditions of the paper points (p > 0.05). Furthermore, no significant effect (p>0.05) on the absorption capacity of these materials was detected in the sterilization test. Conclusion: Contamination was observed in the paper points used by the students, confirming the importance of implementing sterilization protocols. The sterilization protocols implemented in this study did not affect the absorption capacity of the points.KeywordsMicrobiology, Contamination; Bacteria; Anaerobic.

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Section: Introductionmentioning

confidence: 99%

Contamination of paper points used by students during preclinical and clinical endodontic procedures

Angarita

2020

BDS

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“…The success of endodontic therapy is strictly related to the careful aseptic performance of all its phases, including opening, cleaning, disinfecting, modeling and subsequent sealing of the entire root canal system 13 . However, during this process there may be failures in instrumentation, infiltration of bacteria present in saliva and/or use of contaminated instruments and/or materials 4 . Microorganisms may still persist after biomechanical preparation due to variations in root canal internal anatomy or as a result of the defense mechanisms…”

Section: Introductionmentioning

confidence: 99%

Decontamination of Gutta-percha Cones employed in Endodontics.

Carvalho

Pinto

Batista

et al. 2020

Acta Odontol Latinoam

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The gutta-percha cones used in endodontic treatment are produced in aseptic conditions and their composition includes zinc oxide, which is responsible for antibacterial activity. However, there is the possibility of microbial contamination by manipulation, aerosol or during storage. Although several chemical agents have been tested for their decontamination, there is no consensus on the best disinfection protocol to be used. The aim of this study was to evaluate the decontamination of gutta-percha cones contaminated with the bacteria Enterococcus faecalis, by using chlorhexidine digluconate (CHX) and sodium hypochlorite (NaClO) at different concentrations for short exposure times. For this purpose, gutta-percha cones (size 40) were selected at random from a sealed box and immersed for 1 min in a microbial suspension. Then they were immersed in specific Petri dishes for different groups containing: CHX 2%, NaClO 1% or NaClO 2.5% for 30 s or 1 min, and subsequently placed in tubes containing BHI broth. After incubating the tubes for 48 h, it was observed that 1% and 2.5% NaClO and 2% CHX were effective for decontaminating the cones at those exposure time intervals. Microbial growth was detected in one of the replicates of the group with CHX applied for 30 s. To prevent the possibility of failures at this stage, the

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“…More recently, these manufacturers provided paper points packed in until 5 units, and they ensure their sterility. However, several studies tested the sterility of these cones and the contamination was proved through bacterial growth 7,13,15,16 .…”

Section: Introductionmentioning

confidence: 99%

Microbiological analysis of absorbent paper points employed by undergraduate students from a dental school in south of Brazil

Silva¹,

Só²,

Só³

et al. 2017

J. res. dent.

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Aim:The aim of this study was identify contamination on absorbent paper points used by students of Dental Clinic III of the Faculty of Dentistry of Federal University of Rio Grande do Sul in the semester 2015/1, in order to warn students and professionals of the area on the importance to sterilize these materials. Material and Methods:In a clinical environment, 180 absorbent paper points we collected (80 of them from the first series and 80 from the second one), from 40 students. After the collection, each one was singly immersed in a microtube containing 1.5 ml of the BHI (Brain Heart Infusion) culture. Positive control was composed by one paper point contaminated by saliva, and negative control was composed by a closed microtube, only with BHI. The microtubes were incubated at 37ºC in bacteriological incubator during 14 days. The microtubes that presented turbidity were considered positive, and those which did not present turbidity were considered negative. Results:The results were analyzed by the Fisher Exact Test, which demonstrated that paper points from the second series presented higher agreement contamination between the paper points collected from each box, when compared to the analyzed boxes from the first series (p=0.03). All the samples observed presented growth of Bacillus spp in the microorganism identification. Conclusions:It is possible conclude that absorbing paper points, when exposed to clinical environment suffer contamination, and the autoclave sterilization is necessary before the use, regardless the commercial brand, in order to ensure the aseptic chain maintenance.

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Analysis of contamination of endodontic absorbent paper points

Cited by 3 publications

References 1 publication

Contamination of paper points used by students during preclinical and clinical endodontic procedures

Contamination of paper points used by students during preclinical and clinical endodontic procedures

Decontamination of Gutta-percha Cones employed in Endodontics.

Microbiological analysis of absorbent paper points employed by undergraduate students from a dental school in south of Brazil

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