Analysis of Constituents from Guibi-Tang with Lactobacillus

Abstract

Abstract-Guibi-tang (GB) is a traditional medicine used for anti-oxidant, anti-osteoporosis, hemostasis and gastroprotection. We investigated the levels of several compounds in GB before and after fermented with Lactobacillus curvatus KFRI 166 (GB166) and Lactobacillus plantarum KFRI 442 (GB442). The characterization of seven compounds were achieved by comparing the HPLC retention times (t R ) and UV absorptions of target peaks in the GB with those of standards liquiritin (1), nodakenin (2), nodakenitin (3), decursinol (4), 6-gingerol (5), decursinol angelate (6) and decursin (7). We are special ingredients of GB166 and GB442 were compared. As a result, the five compo-unds with the GB166 was decreased by L. curvatus KFRI 166, and the other nodakenin (2) and decursinol (4) were fermentation selective increased. But, GB442 was all compounds decreased. GB fermented with Lactobacillus affects the content of several compounds will be improves its absorption for develop the bioactivity. Index Terms-Guibi-tang, fermented, analysis, HPLC I. INTRODUCTIONMany traditional herbal medicines are used for the prevention and therapeutic treatment of diseases [1]. Indeed, many herbal therapies have become increasingly popular among patients and physicians, and they are now used by approximately 20% of the population in the United States because herbal medicines generally have few side effects and they are very effective [2]. However, concerns have been raised over the lack of quality control and scientific evidence for the safety of herbal medicine [3]. Besides, few scientific studies have explored the safety and toxicity of herbal medicines, so severalwa-rnings have been issued regarding the potential adverse effects of herbal medicines [4], [5].Guibi-tang (GB) is a multi-herbal traditional Korean medicine that has been used for several hundred years to treat amnesia, poor memory or forgetfulness, fatigue, insomnia, anemia, palpitations, and neurosis. GB is composed of 12 herbs: Angelica gigas Nakai, of the active components from herbs as well as increase their bioactivity. Research on the effect of fermented with microorganisms on the quality and efficacy of medicinal herbs was conducted recently [6]- [8].In this study, we fermented GB with Lactobacillus, which is widely used as a food material. Lactobaci-llus is known to inhibit the growth of some harmful bacteria by the production of lactic acid, and it has therapeutic effects, including anti-inflammatory and anti-cancer activities. To determine the changes of compounds in GB after fermentation, seven marker compounds, liquiritin (Glycyrrhiza uralensis Fischer), nodakenin (Angelica gigas Nakai), nodakenitin (Ang-elica gigas Nakai), decursinol (Angelica gigas Nakai), 6-gingerol (Zingiber officinale Roscoe), decursinol angelate (Angelica gigas Nakai) and decursin (Angelica gigas Nakai) were investingated. Amounts of the seven marker compounds in GB and fermented with Lactobacillus curvatus KFRI 166 (GB166) and Lactobacillus plantarum KFRI 442 (GB442) were measured by an establ...

Effect of Guibi-Tang, a Traditional Herbal Formula, on Retinal Neovascularization in a Mouse Model of Proliferative Retinopathy

“…GBT contains 12 herbal medicines, and its ingredient compounds are two flavonoids (liquiritin and glycyrrhizin), a phenolic (6-gingerol) and five coumarins (nodakenin, nodakenetin, decursinol, decursinol angelate, and decursin) [ 52 , 53 ]. Glycyrrhizin has anti-angiogenic activities such as endothelial cell migration, invasion and tube formation, along with anti-tumor activities such as angiogenesis and tumor growth in mice [ 54 ].…”