Analysis of composition, morphology, and biosynthesis of cuticular wax in wild type bilberry (Vaccinium myrtillus L.) and its glossy mutant

Trivedi, Priyanka; Nguyen, Nga; Kļaviņš, Linards; Kviesis, Jorens; Heinonen, Esa; Remes, J.; Jokipii-Lukkari, Soile; Kļaviņš, Māris; Karppinen, Katja; Jaakola, Laura; Häggman, Hely

doi:10.1016/j.foodchem.2021.129517

Cited by 26 publications

(22 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the berries of the three cultivars showed almost similar cuticular wax load (Figure 2), the results imply that the difference between glaucous and glossy appearances of grape berry may base on the cuticular wax morphology but not the wax load. Similar findings were found in the glossy mutant of bilberry (13).…”

Section: The High Density Of Irregular Lamellar Crystal Structures Was Correlated With the Glaucous Appearances Of Grape Berrysupporting

confidence: 87%

“…Previous studies of Arabidopsis leaf and stem with glaucous phenotypes showed high wax load accompanied by high density of cuticular wax crystals ( 22 ). The glossy fruits of citrus ( 14 ), cucumber ( 23 ), and bilberry ( 13 ) showed a reduction in cuticular wax load and crystalloids. In the present study, we found that glaucous appearing “Kyoho” and “Red Globe” berries contained a highly dense cover of platelets along with lamellar-like structures, while glossy appearing “Shine Muscat” berries had limited crystals throughout the fruit development ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

“…These include APETALA2/ethylene responsive factor binding proteins WAX INDUCER1 (WIN1) and SHINE1 (SHN1) ( 8 ), ABA-mediated MYB protein MYB96 ( 9 ), and AP2/ERF protein DEWAX ( 10 ). Studies of cuticular wax biosynthesis have focused on some fruit crops, such as tomato ( 11 ), pear ( 12 ), bilberry ( 13 ), and citrus ( 14 , 15 ), but research on grape berry is very limited.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative Analysis of Cuticular Wax in Various Grape Cultivars During Berry Development and After Storage

Zhang

et al. 2021

Front. Nutr.

View full text Add to dashboard Cite

Cuticular wax covering the surface of fleshy fruit is closely related to fruit glossiness, development, and post-harvest storage quality. However, the information about formation characteristics and molecular mechanisms of cuticular wax in grape berry is limited. In this study, crystal morphology, chemical composition, and gene expression of cuticular wax in grape berry were comprehensively investigated. Morphological analysis revealed high density of irregular lamellar crystal structures, which were correlated with the glaucous appearances of grape berry. Compositional analysis showed that the dominant wax compounds were triterpenoids, while the most diverse were alkanes. The amounts of triterpenoids declined sharply after véraison, while those of other compounds maintained nearly constant throughout the berry development. The amounts of each wax compounds varied among different cultivars and showed no correlation with berry skin colors. Moreover, the expression profiles of related genes were in accordance with the accumulation of wax compounds. Further investigation revealed the contribution of cuticular wax to the water preservation capacity during storage. These findings not only facilitate a better understanding of the characteristics of cuticular wax, but also shed light on the molecular basis of wax biosynthesis in grape.

show abstract

Section: The High Density Of Irregular Lamellar Crystal Structures Was Correlated With the Glaucous Appearances Of Grape Berrysupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Analysis of Cuticular Wax in Various Grape Cultivars During Berry Development and After Storage

Zhang

et al. 2021

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…Our results suggest that the switch between β-amyrin and oleanolic acid could be majorly caused due to the variation in environmental conditions. In our previous study (Trivedi et al, 2019a), where wax samples were collected in Oulu, Finland in 2017, β-amyrin was the dominant triterpenoid, while in this study, although berries were collected from the same locations in 2018 (Trivedi et al, 2021), oleanolic acid was the dominant triterpenoid. Therefore, it seems that the dominant triterpenoid compound changes, especially oleanolic acid levels, are more affected by the changing environmental factors and less by the genetic factors.…”

Section: Profiles Of Very Long Chain Fatty Acid and Triterpenoids Sho...mentioning

confidence: 53%

Temperature has a major effect on the cuticular wax composition of bilberry (Vaccinium myrtillus L.) fruit

Trivedi

Kļaviņš²,

Hykkerud³

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Cuticle is the first layer protecting plants against external biotic and abiotic factors and is responsive to climatic factors as well as determined by genetic adaptations. In this study, the chemical composition of bilberry fruit cuticular wax was investigated through a latitudinal gradient from Latvia (56°N 24°E) through Finland (65°N 25°E) to northern Norway (69°N 18°E) in two seasons 2018 and 2019. Changes in the major cuticular wax compounds, including triterpenoids, fatty acids, alkanes, aldehydes, ketones, and primary alcohols, were detected by GC-MS analysis. Generally, a decreasing trend in the proportion of triterpenoids from southern to northern latitudes, accompanied with an increase in proportion of fatty acids, aldehydes, and alkanes, in bilberry fruit cuticular wax was observed. A correlation analysis between climatic factors with proportion of wax compounds indicated that temperature was the main factor affecting the cuticular wax composition in bilberries. A controlled phytotron experiment with southern and northern bilberry ecotypes confirmed the major effect of temperature on bilberry fruit cuticular wax load and composition. Elevated temperature increased wax load most in berries of northern ecotypes. The level of triterpenoids was higher, while levels of fatty acids and alkanes were lower, in wax of bilberry fruits ripened at 18°C compared to 12°C in both northern and southern ecotypes. Based on our results, it can be postulated that the predicted increase in temperature due to climate change leads to alterations in fruit cuticular wax load and composition. In northern ecotypes, the alterations were especially evident.

show abstract

“…This is one possible explanation for the taeniid eggs not being able to attach as easily or persistently to lingonberries compared with bilberries. Chemical composition of the wax affects epicuticular morphology in bilberry and wild type bilberries have dense rod-like formations and platelets in all of the latest developmental stages ( Trivedi et al, 2021 ). To the authors' knowledge there are no studies describing whether parasitic eggs or other pathogens could be embedded in the waxy layer during ripening, but if eggs could be buried in the waxy layer during ripening, they would not be washed off from the berry surface easily.…”

Section: Discussionmentioning

confidence: 99%

Dispersal of taeniid eggs: Experimental faecal contamination of forest environment followed by DNA detection in wild berries

Malkamäki

Oksanen

Näreaho

et al. 2022

Food and Waterborne Parasitology

View full text Add to dashboard Cite

Analysis of composition, morphology, and biosynthesis of cuticular wax in wild type bilberry (Vaccinium myrtillus L.) and its glossy mutant

Cited by 26 publications

References 36 publications

Comparative Analysis of Cuticular Wax in Various Grape Cultivars During Berry Development and After Storage

Comparative Analysis of Cuticular Wax in Various Grape Cultivars During Berry Development and After Storage

Temperature has a major effect on the cuticular wax composition of bilberry (Vaccinium myrtillus L.) fruit

Dispersal of taeniid eggs: Experimental faecal contamination of forest environment followed by DNA detection in wild berries

Contact Info

Product

Resources

About