Analysis of Component Se-containing Proteins in Se-enriched Rice Using Size Exclusion Chromatography-Inductively Coupled Plasma Mass Spectrometry

Fang, Yong; Yang, Wenjian; Ma, Ning; Tang, Xiaohong; Chen, Xi; Hu, Qiu-Hui

doi:10.3724/sp.j.1096.2013.20845

Cited by 1 publication

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“…We investigated the compositions of Se‐containing proteins in Se‐enriched rice by size exclusion chromatography (SEC)‐HPLC‐ICP‐MS. We found that Se was mainly incorporated into glutelin and globulin but not incorporated into albumin or prolamin, and the major Se‐binding glutelin fraction had a molecular weight of 200 kDa (Fang et al., 2013). Direct analysis of biomacromolecules is very complicated, and the relatively low abundance of Se amplifies the difficulty of characterizing selenoproteins.…”

Section: Analysis Of Selenium: From Total Amount To Speciationmentioning

confidence: 99%

Selenium in cereals: Insight into species of the element from total amount

Xie

Sun

et al. 2021

Comp Rev Food Sci Food Safe

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Selenium (Se) is a trace mineral micronutrient essential for human health. The diet is the main source of Se intake. Se‐deficiency is associated with many diseases, and up to 1 billion people suffer from Se‐deficiency worldwide. Cereals are considered a good choice for Se intake due to their daily consumption as staple foods. Much attention has been paid to the contents of Se in cereals and other foods. Se‐enriched cereals are produced by biofortification. Notably, the gap between the nutritional and toxic levels of Se is fairly narrow. The chemical structures of Se compounds, rather than their total contents, contribute to the bioavailability, bioactivity, and toxicity of Se. Organic Se species show better bioavailability, higher nutritional value, and less toxicity than inorganic species. In this paper, we reviewed the total content of Se in cereals, Se speciation methods, and the biological effects of Se species on human health. Selenomethionine (SeMet) is generally the most prevalent and important Se species in cereal grains. In conclusion, Se species should be considered in addition to the total Se content when evaluating the nutritional and toxic values of foods such as cereals.

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Section: Analysis Of Selenium: From Total Amount To Speciationmentioning

confidence: 99%