Analysis of Coat Proteins from Group IV RNA Coliphages

Hirashima, Akikazu; Harigai, Harumi; Watanabe, Itaru

doi:10.1111/j.1348-0421.1982.tb00258.x

Cited by 1 publication

(1 citation statement)

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“…Its function is less clear, although it has been shown to be essential for the formation of infectious particles (18). The coat protein gene in SP was identified on the basis of known carboxy and aminoterminal sequences (19). The Al protein was identified as being the next large open reading frame downstream from the coat protein.…”

Section: Determination Of the Complete Nucleotide Sequence Of The Sp mentioning

confidence: 99%

Analysis of the complete nucleotide sequence of the group IV RNA coliphage SP

Inokuchi

Jacobson

Hirose

et al. 1988

Nucleic Acids Research

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We report the nucleotide sequence of the Group IV RNA bacteriophage SP. The entire sequence is 4276 nucleotides long. Four cistrons have been identified by comparison with the related Group III phage Q beta. The maturation protein contains 449 amino acids, the coat protein contains 131 amino acids, the read-through protein contains 330 amino acids and the replicase beta-subunit contains 575 amino acids. SP is 59 nucleotides longer than Q beta. We have analyzed both sequence and structural conservation between SP and Q beta and shown that the sequences for the coat and central region of the replicase are strongly conserved between the two genomes. We also show that the S and M replicase binding sites of Q beta are strongly conserved in SP. Interestingly, the base composition of SP and Q beta differ significantly from one another, and most of the differences can be accounted for by a strong preponderance of U in the third position of each codon of Q beta relative to SP. We also compare conserved hairpins associated with potential coat protein and replicase binding sites.

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Section: Determination Of the Complete Nucleotide Sequence Of The Sp mentioning

confidence: 99%