Analysis of clinical-epidemiological, laboratory parameters and cytokine status in patients with erythematous and non-erythematous forms of Ixodes tick borreliosis

Бондаренко, А. Л.; Sapozhnikova, Veronika V.

doi:10.20953/1729-9225-2018-2-34-42

Cited by 5 publications

(2 citation statements)

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“…По данным литературы известно, что большинство больных микст-инфекцией ИКБ и КЭ, а также пациентов с моноинфекцией ЛФ КЭ или ИКБ имеют умеренное увеличение относительного количества палочкоядерных и сегментоядерных нейтрофилов, снижение уровней лимфоцитов и числа тромбоцитов с последующей нормализацией этих показателей в период реконвалесценции [6,7,13,14]. По-видимому, такие изменения гемограммы являются отражением иммунного ответа и сдвигов системы гомеостаза при смешанной инфекции двух возбудителей ИКБ и КЭ [23].…”

Section: результаты исследования и обсуждениеunclassified

“…Кроме того, в литературе имеется ряд данных о различиях показателей гемограммы и лейкоцитарных индексов у больных эритемной формой (ЭФ) и БЭФ моноинфекции ИКБ [6][7][8], но крайне мало информации об особенностях изменений показателей общеклинического анализа крови у больных микст-инфекцией ИКБ и КЭ [9,10].…”

Section: Introductionunclassified

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Predictive value of complete blood count for early diagnosis of mixed infection of the non-erythema migrans form of Lyme borreliosis and tick-borne encephalitis

Ilyinskikh

Filatova

Bulankov

et al. 2022

jour

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Objective: is to develop a model for early diagnosis of mixed infection of the non-erythema migrans form of Lyme borreliosis and tick-borne encephalitis using the assessment of the complete blood count and the blood leukocyte indices of patients in the first week of the disease.Materials and methods. The retrospective clinical study involved Group 1 of 27 patients with the mixed infection of the non-erythema migrans form of Lyme borreliosis and the febrile form of tick-borne encephalitis and Group 2 of 29 patients with the monoinfection of the non-erythema migrans form of Lyme borreliosis, who were hospitalized no later than in the 7th day of the disease. The average age of patients in Groups 1 and 2 was 50.6±3.4 and 49.9±2.3. We analyzed 14 parameters of the complete blood count as well as calculated the leukocyte intoxication index and the body resistance index. Statistical significance assessment was carried out using the chi-square test and ROC analysis. Logistic regression model was developed using STATISTICA 12.0 modules.Results. The levels of the band and polymorphonuclear neutrophils (<0,001 и p=0,002) and the leukocyte intoxication index (p<0,001) were significantly higher and the levels of the body resistance index (p<0.001), lymphocytes (p<0.001) and platelets (p=0.004) were lower in Group 1 than in Group 2. Informative predictors of mixed infection included the body resistance index (AUC=0.77), leukocyte intoxication index (AUC=0.75), the band and polymorphonuclear neutrophils (AUC=0.74), lymphocytes (AUC=0.77), and platelets (AUC=0.70). Logistic regression model has a “very good” predictive value (AUC=0.85) and include two parameters: body resistance index and platelets (×109/L).Conclusion. The developed model has a “very good” predictive value for early diagnosis of the mixed infection of the non-erythema migrans form of Lyme borreliosis and tickborne encephalitis before laboratory diagnosis confirmation.

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Section: результаты исследования и обсуждениеunclassified

Section: Introductionunclassified

Predictive value of complete blood count for early diagnosis of mixed infection of the non-erythema migrans form of Lyme borreliosis and tick-borne encephalitis

Ilyinskikh

Filatova

Bulankov

et al. 2022

jour

View full text Add to dashboard Cite

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Features of baseline and lipopolysaccharide-induced cytokine secretion in mononuclear leukocyte cultures from patients with the erythema migrans form of acute lyme borreliosis based on clinical parameters

Ilyinskikh,

Voronkova,

Hasanova

et al. 2024

Russian Journal of Infection and Immunity

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Introduction. Features of cytokine production in mononuclear cell cultures from Lyme borreliosis patients based on clinical data remained poorly studied. The study aim was to estimate the patterns of baseline and lipopolysaccharide-induced cytokine-secretory activity of peripheral blood mononuclear leukocytes from patients with erythema migrans form of acute Lyme borreliosis based on clinical parameters. Materials and methods. Groups of 22 and 12 patients with the diagnoses of mild or moderate severity of monoinfection and co-infection with tick-borne encephalitis of Lyme borreliosis with erythema migrans were examined twice: on week 1 after disease onset and day 14. The control group included 17 healthy donors. Basal and lipopolysaccharide-induced IL-6, IL-10, and TNFα secretion levels were assessed in mononuclear leukocyte culture supernatants applying enzyme immunoassay. Statistical analysis was performed by using the Mann–Whitney U-test, Wilcoxon test, and Spearman’s rank correlation. Results. The group of moderate severity patients was clinically distinguished by severer fever and intoxication manifestations. At the disease onset, the basal TNFα, IL-6 and IL-10 secretion levels in the moderate severity patient cultures were significantly higher than in those of the other groups. After antibiotics treatment, the baseline TNFα and IL-10 levels tended to decrease. At the onset, lipopolysaccharide-induced cultures from the moderate severity patients showed significantly suppressed TNFα production and increased IL-10 secretion as compared to the other groups. Lipopolysaccharide-induced IL-6 secretion in the moderate vs. mild severity group supernatants was significantly lower. In dynamics, the induced TNFα levels in the moderate severity patients were increased to the magnitude exceeding that in the controls. Positive correlations between the IL-6 and TNFα basal levels and maximum body temperature or the C-reactive protein serum concentrations were revealed in the patients. Induced TNFα levels showed negative correlations with fever levels or with IL-10 secretion. Conclusions. It was demonstrated that basal TNFα, IL-6 and IL-10 secretion levels in the mononuclear cell cultures of acute Lyme borreliosis patients increased with the increasing disease severity. Suppression of lipopolysaccharide-induced TNFα production in the moderate severity patient cultures was presumably associated with the regulatory cytokine IL-10 effects.

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Applying decision tree algorithms to early differential diagnosis between different clinical forms of acute Lyme borreliosis and tick-borne encephalitis

Ilyinskikh,

Filatova,

Samoylov

et al. 2023

Epidemiology and Infectious Diseases

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Background: Tick-borne encephalitis and Lyme borreliosis are the most common natural focal infections in Russia often arising as a mixed infection, which is often clinically difficult to distinguish from a monoinfection at the onset of the disease that is a result of delayed laboratory verification of the diagnosis and it requires further searching for fundamentally new approaches to the issue of early differential diagnosis of tick-borne infections.Aims: is to develop decision tree algorithms for early differential diagnosis between the mono- and mixed forms of acute Lyme borreliosis and tick-borne encephalitis with prevailing febrile syndrome in clinical picture based on clinical and laboratory data.Materials and methods: We retrospectively analyzed 55 clinical and laboratory parameters obtained from 291 hospitalized tick-borne infections patients with or without erythema migrans at the site of ixodid tick bites in the first week of the disease, who were included in the single-center study from 2010 to 2023. In 211 patients without erythema, the analysis was carried out between three classes depending on the diagnosis: the mixed infection of non-erythematous Lyme borreliosis and tick-borne encephalitis, the mono-infection of non-erythematous Lyme borreliosis or the monoinfection of tick-borne encephalitis. The other two classes, which included 80 patients with erythema, had the mixed infection of acute erythematous Lyme borreliosis and tick-borne encephalitis or erythematous Lyme borreliosis monoinfection. Python programming language was applied to develop two decision tree models. Feature importance was assessed for all predictors. Each patient class was randomly divided into training (70%) and testing (30%) datasets. Accuracy evaluation of the models was based on ROC analysis.Results: The decision tree algorithm for early differential diagnosis among the tick-borne infection patients without erythema migrans included the following most important predictors: maximal fever rise, chills, neutrophil-to-monocyte ratio, ESR, absolute number of reactive lymphocytes and immature granulocytes, and percentage of eosinophils. The model for differential diagnosis between the patients with erythema migrans included the following predictors: maximal fever rise, the absolute number of reactive lymphocytes and immature granulocytes, and the percentage of basophils. Both decision tree models showed excellent predictive values based on sensitivity, specificity, precision, accuracy, and F1 scores, as well as areas under the ROC curve, which were higher than 0.90.Conclusions: Based on clinical and laboratory parameters, two decision tree models with high sensitivity have been developed, which can be easily applied in clinical practice for early differential diagnosis of the tick-borne infections with prevailing fever syndrome.

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Analysis of clinical-epidemiological, laboratory parameters and cytokine status in patients with erythematous and non-erythematous forms of Ixodes tick borreliosis

Cited by 5 publications

References 0 publications

Predictive value of complete blood count for early diagnosis of mixed infection of the non-erythema migrans form of Lyme borreliosis and tick-borne encephalitis

Predictive value of complete blood count for early diagnosis of mixed infection of the non-erythema migrans form of Lyme borreliosis and tick-borne encephalitis

Features of baseline and lipopolysaccharide-induced cytokine secretion in mononuclear leukocyte cultures from patients with the erythema migrans form of acute lyme borreliosis based on clinical parameters

Applying decision tree algorithms to early differential diagnosis between different clinical forms of acute Lyme borreliosis and tick-borne encephalitis

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