Analysis of clearance of intra-arterially administered labels from skeletal muscle

Hyman, Chester; Lenthall, Jim

doi:10.1152/ajplegacy.1962.203.6.1173

Cited by 10 publications

(3 citation statements)

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“…In the cremaster preparation, however, the channels through which circulation persists in the resting state lie mainly parallel to the muscle bundles; they can be traced through from terminal arterioles to collecting venules and have a diameter little or not at all wider than that ofneighbouring capillaries when these are dilated. From clearance studies in cat and rabbit muscle, Hyman & Lenthall (1962) conclude that in resting muscle nutritional blood flow is restricted to special preferential capillary channels permanently open.…”

Section: Discussionmentioning

confidence: 99%

Direct observation of skeletal muscle blood vessels (rat cremaster)

Grant¹

1964

The Journal of Physiology

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The recent description of the rat's cremaster muscle by Majno, Palade & Schoefl (1961) suggested that this muscle might provide a suitable site for direct observation of skeletal muscle blood vessels in the living animal. This proved to be so and the method developed allows the study of the reactions of the vessels to various stimuli applied topically and intravenously. It is thought that the vessels are seen in a state that, for a time at least, is nearly normal for resting skeletal muscle. The only comparable preparations that I can trace are those of Zweifach & Metz (1955a, b) and Hyman & Paldino (1962), but their observations do not cover the same ground as those now reported.The observations are directed more to exploring the possibilities of the method than to solving particular problems. They show, however, that in general the vascular reactions, in terms of dilatation and constriction, are in keeping with those derived from blood flow studies in man and other animals. They also show that the dilatation resulting from the intravenous administration of adrenaline affects mainly the arteries, as with acetylcholine, rather than the minute vessels, as with histamine. METHODS Animals. A rat of about 100 g body weight is anaesthetized with urethane (150 mg) sometimes with pentobarbitone sodium (3-0 mg) injected intraperitoneally, and placed on its back on a cork mat with the hind legs widely splayed. Body temperature is regulated by an electric lamp placed above and moved nearer or further away as required. Temperatures are measured by a thermo-electric thermometer (copper-constantan junctions); body temperature from a polythene covered junction inserted into the colon about 5 cm beyond the anus, tail temperature (when required) by a junction attached near its tip with zinc oxide plaster; that of the fluid irrigating the exposed muscle by a junction attached to the tip of the irrigating tube (Text- fig. 2).Blood pressure is measured (when required) from the carotid artery through a polythene tube, 0-58 mm internal diameter (PE 50, Clay Adams Inc., New York) and about 4 cm long, joined by a 3 mm (about) internal diameter tube (PE 330) to a miniature membrane manometer like that described by Sherrington (1919). Ringer's solution with heparin (10 u./ml.) perfused through the manometer at a rate of 0 5-1 0 ml./hr prevents clotting in the tube tip.

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Section: Discussionmentioning

confidence: 99%

Direct observation of skeletal muscle blood vessels (rat cremaster)

Grant¹

1964

The Journal of Physiology

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“…In seven of these a decrease in clearance rate was observed in association with and roughly proportional to the degree of depression of muscle force (figs. [4][5][6]; in one case no change in clearance occurred. On the other hand, comparable reduction in muscle force produced by d-tubocurarine, or complete rest of the muscle by stopping the stimulation ("muscle off" in fig.…”

Section: I"' Clearance From Muscle During Acetylcholine or D-tubocuramentioning

confidence: 94%

Depression of Contractile Force of Skeletal Muscle by Intra-arterial Vasodilator Drugs

Hirvonen

Korobkin

Sonnenschein

et al. 1964

Circulation Research

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In a previous study 1 the maximum force of contraction of the intermittently stimulated gastrocnemius-soleus muscle group of the cat was shown to decrease in proportion to reduction in total blood flow through the muscle, brought about by various means. Activation of the cholinergic sympathetic vasodilator system through hypothalamic stimulation produced neither change in flow nor alteration in force of the contracting muscle; this suggested that the dilator system produced no redistribution of flow in contracting muscle similar to that observed in resting muscle. 2 As a corollary of these observations the effect on flow-force relations of the intra-arterial administration of acetylcholine and other vasodilator agents was investigated. As indicated in preliminary reports, 3 ' 4 these agents reduced muscle force even when flow was increased or unchanged. The mechanism of this effect is the subject of the present report. MethodsCats of either sex, weighing 2.0 to 3.0 kg, were anesthetized, after initial etherization, with alpha-chloralose (50 mg/kg iv), supplemented with urethane as required. After one hind leg was skinned, the artery supplying the gastrocnemius-soleus muscle group was isolated; in a few experiments the corresponding vein was isolated instead. Collateral circulation from thigh muscles was eliminated by ligating and sectioning them close to their insertions. The tibialis muscles were removed and the paw was ligated to eliminate its flow. Other details of this "isolated"

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“…Studies of clearance rates of radioactive isotopes from tir,' ga.s-trocnemius muscle in man (Walder,19.53) and in eats and rabbits (llyman, 1957: Hyman andLenthall, 1962), however, have provided e\ldenee snppiirting t)u' concept of two inde^x-ndent functional circulations in skeletal mnsele.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Decreased Muscle Blood Flow on Post‐exercise Hyperaemia in the Human Forearm

Roberts

et al. 1965

Aust J Exp Biol Med

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Summary. Post-exercise hyppraemia following "no, two or throe minute poHwls of forearm t'sercist-was .studied in man, Ix-forc and after tilting retiitnlioiit subjc-cts to 45° in tlw feet down position. Tilting rcditces forearm muscle blood How, due to a svinpatlictic vasiKcoiistrittor reflc.v.The niURnitnde and tlic duration of post-exerci.se liyporaeniia were signifieantly rcclitced hy tilting. Peak blooci fl(tw.s were also deerrased, but lliis trend wa.s significant only for the one minute periotl of exerd.Sf. It i.s eoneluded that if two functional circulations exist in skeletal muscle, they do not have independent regnlation. INTRODUCTION.

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Analysis of clearance of intra-arterially administered labels from skeletal muscle

Cited by 10 publications

References 0 publications

Direct observation of skeletal muscle blood vessels (rat cremaster)

Direct observation of skeletal muscle blood vessels (rat cremaster)

Depression of Contractile Force of Skeletal Muscle by Intra-arterial Vasodilator Drugs

The Effects of Decreased Muscle Blood Flow on Post‐exercise Hyperaemia in the Human Forearm

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