Analysis of classical neurotransmitter markers in tapeworms: Evidence for extensive loss of neurotransmitter pathways

Preza, Matías; Montagne, Jimena; Costábile, Alicia; Iriarte, Andrés; Castillo, Estela; Koziol, Uriel

doi:10.1016/j.ijpara.2018.06.004

Cited by 14 publications

(18 citation statements)

References 115 publications

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“…Altogether, the high-throughput sequence data generated recently from an essential, broadly accessible pool of data to benefit from in future explorations into various aspects of parasitic flatworms' biology. Appealing examples from research on taeniid tapeworm models already exist, e.g., the survey of tapeworm nervous system components by Koziol et al (2016) and Preza et al (2018) demonstrating the conservation and/or loss of individual neurotransmitters within this evolutionary lineage. Given the relative state of the genome assembly as well as the availability (and manageability) of its life cycle in the laboratory, S. solidus might be the first among the broad tapeworms to join the established taeniid models and allow for further validating various biological phenomena studied in cestodes.…”

Section: Genomics Of Broad Tapewormsmentioning

confidence: 99%

Broad tapeworms (Diphyllobothriidae), parasites of wildlife and humans: Recent progress and future challenges

Scholz

Kuchta

Brabec

2019

International Journal for Parasitology: Parasites and Wildlife

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Tapeworms of the family Diphyllobothriidae, commonly known as broad tapeworms, are predominantly large-bodied parasites of wildlife capable of infecting humans as their natural or accidental host. Diphyllobothriosis caused by adults of the genera Dibothriocephalus , Adenocephalus and Diphyllobothrium is usually not a life-threatening disease. Sparganosis, in contrast, is caused by larvae (plerocercoids) of species of Spirometra and can have serious health consequences, exceptionally leading to host's death in the case of generalised sparganosis caused by ‘ Sparganum proliferum ’. While most of the definitive wildlife hosts of broad tapeworms are recruited from marine and terrestrial mammal taxa (mainly carnivores and cetaceans), only a few diphyllobothriideans mature in fish-eating birds. In this review, we provide an overview the recent progress in our understanding of the diversity, phylogenetic relationships and distribution of broad tapeworms achieved over the last decade and outline the prospects of future research. The multigene family-wide phylogeny of the order published in 2017 allowed to propose an updated classification of the group, including new generic assignment of the most important causative agents of human diphyllobothriosis, i.e., Dibothriocephalus latus and D. nihonkaiensis . Genomic data of selected representatives have also begun to accumulate, promising future developments in understanding the biology of this particular group of parasites. The list of nominal species of taxonomically most complicated genus Spirometra as well as host-parasite list of 37 species of broad tapeworms parasitising marine mammals (pinnipeds and cetaceans) are also provided.

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Section: Genomics Of Broad Tapewormsmentioning

confidence: 99%

Broad tapeworms (Diphyllobothriidae), parasites of wildlife and humans: Recent progress and future challenges

Scholz

Kuchta

Brabec

2019

International Journal for Parasitology: Parasites and Wildlife

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“…Our work confirms that few neuropeptides in cestodes and other parasitic flatworms show evident similarity to conserved animal neuropeptides, which is paralleled by the large proportion of predicted peptide GPCR receptors in their genome without clear orthologs to receptors in other phyla (Koziol et al, 2016; McVeigh et al, 2018). These differences in their complement of neuropeptides parallel those of their classical neurotransmitter systems, as cestodes appear to have lost the canonical pathways required for neurotransmission mediated by dopamine, gamma‐aminobutyric acid, histamine, octopamine, and tyramine (Preza et al, 2018). The uniqueness of their neurotransmission mechanisms may provide an interesting selection of targets for anthelmintic development, which could target the essential neuromuscular system of these parasites.…”

Section: Discussionmentioning

confidence: 99%

“…In the case of hm-npp-6, an additional asymmetric expression site appeared in the strobila, consisting of a single superficial dorsal cell in the posterior end of each segment (Figure S7). This asymmetry was not present in hm-pc2 expression patterns (hm-pc2 was expressed in both dorsal and ventral sites; Preza et al, (2018)), suggesting that although peptidergic nerve cells are present in equivalent dorsal and ventral regions of the adult, these cell populations produce different neuropeptides. This dorsoventral asymmetry appears during adult development, since activated cysticercoid larvae (i.e.…”

Section: Expression Of Npp Genes In H Microstomamentioning

confidence: 96%

Global analysis of neuropeptides in cestodes identifies Attachin, a SIFamide homolog, as a stimulant of parasite motility and attachment

Preza

Bael

Temmerman

et al. 2022

Journal of Neurochemistry

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Many anthelmintics target the neuromuscular system, in particular by interfering with signaling mediated by classical neurotransmitters. Although peptidergic signaling has been proposed as a novel target for anthelmintics, current knowledge of the neuropeptide complement of many helminth groups is still limited, especially for parasitic flatworms (cestodes, trematodes, and monogeneans). In this work, we have characterized the neuropeptide complement of the model cestode Hymenolepis microstoma.Peptidomic characterization of adults of H. microstoma validated many of the neuropeptide precursor (npp) genes previously predicted in silico, and identified novel neuropeptides that are conserved in parasitic flatworms. Most neuropeptides from parasitic flatworms lack significant similarity to those from other animals, confirming the uniqueness of their peptidergic signaling. Analysis of gene expression of ten npp genes by in situ hybridization confirmed that all of them are expressed in the nervous system and identified cryptic features, including the first evidence of dorsoventral asymmetry, as well as a new population of peripheral peptidergic cells that appears to be conserved in the trematode Schistosoma mansoni. Finally, we characterized in greater detail Attachin, an SIFamide homolog. Although its expression is largely restricted to the longitudinal nerve cords and cerebral commissure in H. microstoma, it shows widespread localization in the larval nervous system of Echinococcus multilocularis and Mesocestoides corti. Exogenous addition of a peptide corresponding to the highly conserved C-terminus of Attachin stimulated motility and attachment of M. corti larvae. Altogether, this work provides a robust experimental foothold for the characterization of peptidergic signaling in parasitic flatworms.

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“…On reproductive aspects of development, other groups are working on mammalian spermatogenesis and meiosis from a molecular point of view (Trovero et al, 2020), sex differentiation and gonadal development in fishes (Vizziano-Cantonnet et al, 2016) and on the neuroendocrine regulation of ovarian innervation in rats (Chávez-Genaro and Anesetti, 2018). On different aspects of molecular developmental biology in invertebrates, we highlight the already mentioned continuing research on parasitic worms such as cestodes (Chalar et al, 2016;Montagne et al, 2019;Preza et al, 2018), and in Drosophila (Bolatto et al, 2015). The annual fishes are a particular case, where several groups at Facultad de Ciencias are contributing to understand the particular developmental stages, the molecular regulation of the diapauses and the functional genomics of the early stages and sexual determination of members of the genus Austrolebias (Arezo et al, 2014;Arezo et al, 2017).…”

Section: The Diaspora and The Return To Democracy: After Pupation A mentioning

confidence: 98%

Building the embryo of Developmental Biology in Uruguay

Zolessi

Berois²,

Brauer³

et al. 2021

Int. J. Dev. Biol.

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In Uruguay, a country with a small population, and hence a small scientific community, there were no classical embryologists as such in the past. From the decade of 1950, however, a cumulus of favorable conditions gave rise to highly active and modern research groups in the fields of cytology and physiology, that eventually contributed to developmental biology. The advent of a long dictatorship between de 1970’s and 1980’s caused two things: a strong lag in local research and the migration of young investigators who learned abroad new disciplines and technologies. The coming back to democracy allowed for the return of some, now as solid researchers, and together with those who stayed, built a previously inexistent postgraduate training program and a globally-integrated academy that fostered diversity of research disciplines, including developmental biology. In the present work we highlight the key contribution of pioneer researchers and the significant role played by academic and funding national institutions in the growth and consolidation of developmental biology in our country.

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Analysis of classical neurotransmitter markers in tapeworms: Evidence for extensive loss of neurotransmitter pathways

Cited by 14 publications

References 115 publications

Broad tapeworms (Diphyllobothriidae), parasites of wildlife and humans: Recent progress and future challenges

Broad tapeworms (Diphyllobothriidae), parasites of wildlife and humans: Recent progress and future challenges

Global analysis of neuropeptides in cestodes identifies Attachin, a SIFamide homolog, as a stimulant of parasite motility and attachment

Building the embryo of Developmental Biology in Uruguay

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