Analysis of circulating-microRNA expression in lactating Holstein cows under summer heat stress

Lee, Jihwan; Lee, Mi Kyung; Son, Jun-Kyu; Lim, Hyeonju; Kim, Euntae; Kim, Dong-Hyun; Ha, Seungmin; Hur, Tai-Young; Lee, Seung Hwan; Choi, Inchul

doi:10.1371/journal.pone.0231125

Cited by 21 publications

(20 citation statements)

References 57 publications

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“…miRNA sequencing in chronic heat stress showed that miR-199b was highly expressed and had the same expression tendency in this study [22]. miR-2284 families were also verified in circulating-miRNA expression in lactating Holstein cows under summer heat stress [23]. It suggested that miR-199b and miR-2284 families were related to heat stress response.…”

Section: Discussionsupporting

confidence: 65%

Small RNA-Seq Analysis Reveals miRNA Expression of Short Distance Transportation Stress in Beef Cattle Blood

Tang

Raza

et al. 2021

Animals

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Transportation is a crucial phase in the beef cattle industry, and the annual losses caused by beef cattle transport stress are substantial. Because of its huge economic losses, such as lower growth rate and even death, long-distance transportation stress has attracted more attention from beef production practitioners because of its huge economic losses. Compared with the long-distance transportation stress, the short-distance transportation stress was ignored for the reason of no obvious symptoms in cattle. Our previous study showed that the disorder of B cell function could be a potential health risk after short-distance transportation. However, the transcriptome details of the changes in the cattle blood after short-distance transportation and the molecular mechanisms for the regulation of the developmental process are not clearly known. In this study, a total of 10 Qinchuan cattle were used to compare the molecular characteristics of blood before and after short-distance transportation. The miRNA-seq showed that 114 differentially expressed miRNAs (DEMs) were found (40 upregulated and 74 downregulated) between two groups before and after transportation. Furthermore, more than 90% of the miRNAs with counts of more than 10 were used to construct a co-expression network by weighted correlation network analysis (WGCNA), and four independent modules were identified. According to their relationship with 30 hub genes, the turquoise module was the key module in this study. The regulator network of hub genes and miRNAs in the turquoise module was constructed by miRNAs targeting genes predicting, and the miRNAs had targeting sites within hub genes that could be identified as hub-miRNAs. Further, it showed that CD40 and ITPKB had the same targeting miRNAs (miR-339a/b), and the newly discovered hub miRNAs filled the gaps in our previous study about the relationship between hub genes in short-distance transportation stress and provided the potential utility for predicting and treatment of short-distance transportation stress in beef cattle.

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Section: Discussionsupporting

confidence: 65%

Small RNA-Seq Analysis Reveals miRNA Expression of Short Distance Transportation Stress in Beef Cattle Blood

Tang

Raza

et al. 2021

Animals

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“…On P13, miR-1246 and mir-23b-3p were found as upregulated in UF 6 . MicroR-1246 has been shown to play essential roles in the regulation of progesterone biosynthesis and corpus luteum 80 and has been related to the GO term post-embryonic development in our study. Reliszko et al also found increased levels of miR-23b in P16 serum samples in the pig 81 .…”

Section: Discussionmentioning

confidence: 60%

Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare

Vegas

Hamdi

Podico

et al. 2022

Sci Rep

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In contrast to other domestic mammals, the embryo-derived signal(s) leading to maternal recognition of pregnancy (MRP) are still unknow in the mare. We hypothesize that these embryonic signals could be packed into uterine extracellular vesicles (uEVs), acting as multi-signal messengers between the conceptus and the maternal tract, and contributing to MRP. To unveil these signals, the RNA and protein cargos of uEVs isolated from uterine lavages collected from pregnant mares (P; day 10, 11, 12 and 13 after ovulation) and cyclic control mares (C; day 10 and 13 after ovulation) were analyzed. Our results showed a fine-tuned regulation of the uEV cargo (RNAs and proteins), by the day of pregnancy, the estrous cycle, and even the size of the embryo. A particular RNA pattern was identified with specific increase on P12 related to immune system and hormonal response. Besides, a set of proteins as well as RNAs was highly enriched in EVs on P12 and P13. Differential abundance of miRNAs was also identified in P13-derived uEVs. Their target genes were linked to down- or upregulated genes in the embryo and the endometrium, exposing their potential origin. Our study identified for first time specific molecules packed in uEVs, which were previously associated to MRP in the mare, and thus bringing added value to the current knowledge. Further integrative and functional analyses will help to confirm the role of these molecules in uEVs during MRP in the mare.

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“…However, the exact function of the released miR-1246 in the target recipient cells under stress conditions needs more investigation. In correlation with GCs functions, predicted target genes of miR-1246 were known to play a role in progesterone biosynthesis regulation [ 45 , 46 ]. Moreover, a significant downregulation of miR-1246 expression was reported in large compared to small bovine follicles [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Adaptive and Biological Responses of Buffalo Granulosa Cells Exposed to Heat Stress under In Vitro Condition

Faheem

Ghanem

Gad

et al. 2021

Animals

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The steroidogenesis capacity and adaptive response of follicular granulosa cells (GCs) to heat stress were assessed together with the underlying regulating molecular mechanisms in Egyptian buffalo. In vitro cultured GCs were exposed to heat stress treatments at 39.5, 40.5, or 41.5 °C for the final 24 h of the culture period (7 days), while the control group was kept under normal conditions (37 °C). Comparable viability was observed between the control and heat-treated GCs at 39.5 and 40.5 °C. A higher release of E2, P4 and IGF-1 was observed in the 40.5 °C group compared with the 39.5 or 41.5 °C groups. The total antioxidant capacity was higher in response to heat stress at 39.5 °C. At 40.5 °C, a significant upregulation pattern was found in the expression of the stress resistance transcripts (SOD2 and NFE2L2) and of CPT2. The relative abundance of ATP5F1A was significantly downregulated for all heat-treated groups compared to the control, while TNFα was downregulated in GCs at 39.5 °C. Expression analyses of stress-related miRNAs (miR-1246, miR-181a and miR-27b) exhibited a significant downregulation in the 40.5 °C group compared to the control, whereas miR-708 was upregulated in the 39.5 and 40.5 °C groups. In conclusion, buffalo GCs exhibited different adaptive responses, to the different heat stress conditions. The integration mechanism between the molecular and secretory actions of the GCs cultured at 40.5 °C might provide possible insights into the biological mechanism through which buffalo GCs react to heat stress.

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Analysis of circulating-microRNA expression in lactating Holstein cows under summer heat stress

Cited by 21 publications

References 57 publications

Small RNA-Seq Analysis Reveals miRNA Expression of Short Distance Transportation Stress in Beef Cattle Blood

Small RNA-Seq Analysis Reveals miRNA Expression of Short Distance Transportation Stress in Beef Cattle Blood

Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare

Adaptive and Biological Responses of Buffalo Granulosa Cells Exposed to Heat Stress under In Vitro Condition

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