Analysis of chromosome aberrations and sister-chromatid exchanges in peripheral blood lymphocytes of workers with occupational exposure to the mancozeb-containing fungicide Novozir Mn80

Jablonická, A; Poláková, Helena; Karelová, J; Vargová, Mária

doi:10.1016/0165-1218(89)90148-1

Cited by 52 publications

(19 citation statements)

References 5 publications

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“…24,25 A statistically significant (P < 0.05) increase in the number of all types of chromatid and chromosome aberrations observed in the exposed subjects, with no regard to their smoking habits (Table 1), may be due to their longterm occupational exposure to a complex pesticide mixture (atrazine, alachlor, cyanazine, 2,4-dichlorophenoxyacetic acid, malathion). Similar genotoxic effects (chromosomal aberrations, sister chromatid exchanges, micronuclei formation) associated with pesticide exposure have been reported previously in many biomonitoring studies, after exposure to specific compounds such as mancozeb 26 or complex mixtures. 7,10,13,16,18,27,29,30 Some of those works 16,31 included a comparison of structural chromosomal aberrations between smokers and non-smokers within Group mean value ± SD 50.13 ± 9.44 * 60.85 ± 18.17 * N = non-smoker; S = smoker.…”

Section: Discussionmentioning

confidence: 64%

Assessment of genome damage in a population of Croatian workers employed in pesticide production by chromosomal aberration analysis, micronucleus assay and Comet assay

Garaj‐Vrhovac

Želježić

2002

J of Applied Toxicology

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The widespread use of pesticides suggests that the evaluation of their genotoxicity should be extended using the different assays available. In the present study we used two standard cytogenetic methods (chromosomal aberration analysis and micronucleus assay) and the Comet assay as a relatively new and powerful technique. The study included 10 workers occupationally exposed to a complex mixture of pesticides (atrazine, alachlor, cyanazine, 2,4-dichlorophenoxyacetic acid, malathion) during their production and 20 control subjects with no history of exposure to any physical or chemical agents. For the chromosomal aberration analysis, whole blood was cultivated for 48 h, whereas for the micronucleus assay, whole blood was cultivated for 72 h. For the comet assay whole blood was embedded in agarose on a microscope slide, lysed with detergent, denaturated and subjected to alkaline electrophoresis. Damage to DNA was evaluated by measuring tail length and calculating the tail moment. A significantly increased number of chromatid and chromosome breaks, as well as the presence of dicentric chromosomes and chromatid exchanges in exposed subjects compared with control subjects (P < 0.05), was found. There was also a statistically significant difference in frequency and distribution of micronuclei between the two groups examined. In the exposed subjects the Comet assay showed a statistically significant (P < 0.001) increase in DNA migration. Results suggest that long-term occupational exposure to pesticides could cause genome damage in somatic cells and therefore may represent a potential hazard to human health.

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Section: Discussionmentioning

confidence: 64%

Assessment of genome damage in a population of Croatian workers employed in pesticide production by chromosomal aberration analysis, micronucleus assay and Comet assay

Garaj‐Vrhovac

Želježić

2002

J of Applied Toxicology

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“…Paraquat Health Advisory (draft)]. However, with the exception of malathion, for which detectable mutations were associated with its exposure in three different types of human culture cells, including white blood cells and lymph cells (42,43), the data are inconsistent and inconclusive (41)(42)(43)(44)(45). Benomyl and mancozeb have been found to be associated with cancer in humans (46,47), but not with leukemia.…”

Section: Pesticides Exposure and Childhood Leukemiamentioning

confidence: 99%

Parental occupational exposure to pesticides and the risk of childhood leukemia in Costa Rica

Monge¹,

Wesseling²,

Guardado³

et al. 2007

Scand J Work Environ Health

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“…Benomyl, Zineb, and Mancozeb), and herbicides (e.g. Paraquat and Linuron), very well known inducers of chromosomal aberrations not only in human peripheral lymphocytes but also in other mammalian cells [Jablonická et al, 1989;Georgieva et al, 1990;Bonatti et al, 1994;Ribas et al, 1997;Soloneski et al, 2001;Chauhan et al, 2007;Das et al, 2007;Kocaman and Topaktaş, 2009]. Years ago, Korystov and co-workers [1999] were unable to reveal Abamectin-induced apoptosis in rat thymocytes.…”

Section: Genotoxicity and Cytotoxicity Of Abamectin And Ivermectinmentioning

confidence: 99%

New Ventures in the Genotoxic and Cytotoxic Effects of Macrocyclic Lactones, Abamectin and Ivermectin

Molinari

Soloneski

Larramendy³

2010

Cytogenet Genome Res

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Abamectin and Ivermectin are 2 closely related members of the Avermectin family of 16-membered macrocyclic lactones derived from the actinomycete Streptomyces avermectinius which exhibit extraordinary anthelmintic activity. They are used worldwide in veterinary and human medicine as well as in agriculture. In the present review we summarized the results published so far for estimating the genotoxicity and cytotoxicity exerted by both compounds in several cellular systems. Although both compounds do not induce in vitro and in vivo gene mutations in either bacterial or mammalian cells, there is no concrete evidence of a clear clastogenic effect exerted both in vitro and in vivo in mammalian cells. However, reports indicating that both anthelmintic agents are able to induce single DNA-strand breaks in vitro and inhibit cell growth either in vitro or in in vivo bioassays, are scarce. Taking into account the similarity of the genotoxicity and cytotoxicity exerted by both antibiotics, and that only Abamectin has been classified so far as a class II toxicity pesticide by the EPA, the necessity of reconsideration for a further hazard evaluation of Ivermectin by an international regulatory agency(ies) is strongly recommended.

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Analysis of chromosome aberrations and sister-chromatid exchanges in peripheral blood lymphocytes of workers with occupational exposure to the mancozeb-containing fungicide Novozir Mn80

Cited by 52 publications

References 5 publications

Assessment of genome damage in a population of Croatian workers employed in pesticide production by chromosomal aberration analysis, micronucleus assay and Comet assay

Assessment of genome damage in a population of Croatian workers employed in pesticide production by chromosomal aberration analysis, micronucleus assay and Comet assay

Parental occupational exposure to pesticides and the risk of childhood leukemia in Costa Rica

New Ventures in the Genotoxic and Cytotoxic Effects of Macrocyclic Lactones, Abamectin and Ivermectin

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