Analysis of Chemokines and Receptors Expression Profile in the Myelin Mutant<i>Taiep</i>Rat

Soto-Rodríguez, Guadalupe; Gonzalez-Barrios, Juan-Antonio; Martínez‐Fong, Daniel; Blanco-Álvarez, Víctor Manuel; Eguibar, José R.; Ugarte, Araceli; Martínez-Pérez, Francisco; Brambila, Eduardo; Peña, Lourdes Millán-Pérez; Pazos-Salazar, Nidia-Gary; Torres-Soto, Maricela; Garcia-Robles, Guadalupe; Tomás-Sanchez, Constantino; León-Chávez, Bertha Alicia

doi:10.1155/2015/397310

Cited by 7 publications

(8 citation statements)

References 51 publications

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“…In the present study, we do not discard the possibility that the loss of body weight and the alterations in organ weight in taiep rats could be the result of the crossbreeding to obtain this animal model, which would have to be evaluated as an independent factor in subsequent analyses. At the moment, we can suggest that the decrease in body weight in our animal model is related to inflammatory alterations such as the down-regulation of TGFβ1 at the central level (Soto-Rodriguez et al, 2015). A decrease in TGFβ1 at the hypothalamic level has been reported to cause an increase in locomotor activity accompanied by increased O2 consumption and CO2 production, along with a reduction in interscapular brown adipose tissue, resulting in weight loss (Mendes et al, 2018).…”

Section: Discussionmentioning

confidence: 80%

“…The name taiep is an acronym of the progressive signs of tremor, ataxia, immobility, epilepsy, and paralysis of the hind limbs (Holmgren et al, 1989;Lunn et al, 1995). The taiep rat exhibits a down-regulation of IL-1b, IL-10, and TGFβ1, as well as astrocytosis at the level of the central nervous system (Leon Chavez et al, 2001;Soto-Rodriguez et al, 2015). In the case of male taiep rats, an increase in kisspeptin immunoreactive cell bodies has been observed at the hypothalamic level accompanied by a decrease in LH secretion compared to Sprague-Dawley rats (Muñoz de la Torre et al, 2022); these alterations could result in changes at the testicular and sperm level.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of sperm motility and testosterone secretion in the taiep myelin mutant, a model of demyelination

Muñoz de la Torre,

Trujillo Hernández,

Eguibar

et al. 2023

Anim. Reprod.

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Presently, demyelinating diseases have been reported to affect the reproductive life of patients who suffer from them, but the progression of the alterations is unknown, especially in men. To better understand these effects, it is necessary to perform studies in animal models, such as the male taiep rat, which exhibits progressive demyelination of the central nervous system, altered kisspeptin expression at the hypothalamic level, and decreased luteinizing hormone, which could alter sperm quality and testicular diameter. Thus, the objective of the present study was to analyze the diameter of the seminiferous tubules, the sperm motility, and the testosterone levels of 90-day-old male taiep rats. The obtained results indicate that male taiep rats show an increase in testicular size accompanied by an increase in the diameter of the seminiferous tubules of the left testicle. There was also a decrease in progressive motility in sperm samples from the left epididymis of male taiep rats compared to the control group, with no changes in serum testosterone concentration. Therefore, we conclude that male taiep rats with central demyelination show altered testicular diameter and decreased motility in sperm from the left side. This type of studies serves as a basis for proposing possible reproductive strategies to improve the fertility and testicular function of men with demyelinating diseases of the central nervous system.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Characterization of sperm motility and testosterone secretion in the taiep myelin mutant, a model of demyelination

Muñoz de la Torre,

Trujillo Hernández,

Eguibar

et al. 2023

Anim. Reprod.

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“…A decrease in CCL1 expression could explain the decline in elimination of Aβ by phagocytosis detected in AD 28. In Taiep rats, an upregulation in CCR8 mRNA and protein were detected at 1 month old, explaining the accumulation of microglial cells in the CNS due to the action of promoting chemotaxis and phagocytosis 29.…”

Section: Discussionmentioning

confidence: 94%

Changes in Chemokines and Chemokine Receptors Expression in a Mouse Model of Alzheimer's Disease

Vallés¹,

Cauli²,

Santonja³

et al. 2019

Int. J. Biol. Sci.

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The amyloid precursor protein plus presenilin-1 (APP/PS1) mice are a frequently-used model for Alzheimer's disease studies (AD). However, the data relevant to which proteins are involved in inflammatory mechanism are not sufficiently well-studied using the AD mouse model. Using behavioral studies, quantitative RT-PCR and Western-blot techniques, significant findings were determined by the expression of proteins involved in inflammation comparing APP/PS1 and Wild type mice. Increased GFAP expression could be associated with the elevation in number of reactive astrocytes. IL-3 is involved in inflammation and ABDF1 intervenes normally in the transport across cell membranes and both were found up-regulated in APP/PS1 mice compared to Wild type mice. Furthermore, CCR5 expression was decreased and both CCL3 and CCL4 chemokines were highly expressed indicating a possible gliosis and probably an increase in chemotaxis from lymphocytes and T cell generation. We also noted for the first time, a CCR8 increase expression with diminution of its CCL1 chemokine, both normally involved in protection from bacterial infection and demyelination. Control of inflammatory proteins will be the next step in understanding the progression of AD and also in determining the mechanisms that can develop in this disease.

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“…Changes in oligodendrocytes number and dynamics are increasingly recognized as important components in the pathogenesis of neurodegenerative disorders. In taiep rats, an animal model for multiple sclerosis, CCL5 and its receptor CCR5 is down-regulated in comparison to Sprague-Dawley rats (Soto-Rodriguez et al, 2015 ). Such reduction has been shown to limit the infiltration of pro-inflammatory macrophages and therefore to prevent demyelination (Glass et al, 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Glial- and Neuronal-Specific Expression of CCL5 mRNA in the Rat Brain

et al. 2018

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Chemokine (C-C motif) ligand 5 (CCL5) belongs to a group of chemokines that play a role in the peripheral immune system, mostly as chemoattractant molecules, and mediate tactile allodynia. In the central nervous system (CNS), CCL5 and its receptors have multiple functions, including promoting neuroinflammation, insulin signaling, neuromodulator of synaptic activity and neuroprotection against a variety of neurotoxins. Evidence has also suggested that this chemokine may regulate opioid response. The multifunctional profile of CCL5 might correlate with its ability to bind different chemokine receptors, as well as with its unique cellular expression. In this work, we have used fluorescence in situ hybridization combined with immunohistochemistry to examine the expression profile of CCL5 mRNA in the adult rat brain and provide evidence of its cellular localization. We have observed that the highest expression of CCL5 mRNA occurs in all major fiber tracts, including the corpus callosum, anterior commissure, and cerebral peduncle. In these tracts, CCL5 mRNA was localized in oligodendrocytes, astrocytes and microglia. Astrocytic and microglial expression was also evident in several brain areas including the cerebral cortex, caudate/putamen, hippocampus, and thalamus. Furthermore, using a specific neuronal marker, we observed CCL5 mRNA expression in discrete layers of the cortex and hippocampus. Interestingly, in the midbrain, CCL5 mRNA co-localized with tyrosine hydroxylase (TH) positive cells of the ventral tegmental area, suggesting that CCL5 might be expressed by a subset of dopaminergic neurons of the mesolimbic system. The expression of CCL5 mRNA and protein, together with its receptors, in selected brain cell populations proposes that this chemokine could be involved in neuronal/glial communication.

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Analysis of Chemokines and Receptors Expression Profile in the Myelin MutantTaiepRat

Cited by 7 publications

References 51 publications

Characterization of sperm motility and testosterone secretion in the taiep myelin mutant, a model of demyelination

Characterization of sperm motility and testosterone secretion in the taiep myelin mutant, a model of demyelination

Changes in Chemokines and Chemokine Receptors Expression in a Mouse Model of Alzheimer's Disease

Glial- and Neuronal-Specific Expression of CCL5 mRNA in the Rat Brain

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