Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS

Abstract

The ethnic drug Melastoma dodecandrum Lour. (MDL) is widely distributed throughout South China, and is the major component of Gong Yan Ping Tablets/Capsules and Zi Di Ning Xue San. Although the pharmacological effects of MDL have been well documented, its chemical profile has not been fully determined. In this study, we have developed a rapid and sensitive UPLC-ESI-Q-Exactive Focus-MS/MS method to characterize the chemical constituents of MDL in the positive and negative ionization modes. A comparison of the chromatographic and spectrometric data obtained using this method with data from databases, the literature and reference standards allowed us to identify or tentatively characterize 109 compounds, including 26 fatty acids, 26 organic acids, 33 flavonoids, six tannins, 10 triterpenoids, two steroids and six other compounds. Notably, 55 of the compounds characterized in this study have never been detected before in this plant. The information obtained in this study therefore enriches our understanding of the chemical composition of MDL and could be used in quality control, pharmacological research and the development of drugs based on MDL. In addition, this study represents the first reported comprehensive analysis of the chemical constituents of MDL.

Neutrophil elastase inhibitory effects of pentacyclic triterpenoids from Eriobotrya japonica (loquat leaves)

“…The deprotonated ions and MS 2 fragment ions of peaks 3 and 4 were basically the same (Table 2, 633.27/487.06 for peak 3 and 633.15/ 487.12 for peak 4) and consistent with the compound 3-O-p-coumaroyltormentic acid (633.38, 487.34, [M-H-C 9 H 6 O 2 ] -) mentioned in the references (Cao et al, 2016;Tan et al, 2015;Wang et al, 2017;Wu et al, 2013). Peaks 3 and 4 were presumed to be the cis and trans isomers, respectively, of 3-O-p-coumaroyltormentic acid.…”

“…Some studies have reported that PTs are likely to be substrates for P-gp. When a solid dispersion of total PTs in (Cao et al, 2016;Tan et al, 2015;Wang et al, 2017;Wu et al, 2013) Fig. 2.…”

Recent advances and effective strategies in the discovery and applications of natural products

“…Selection of right appropriate research techniques is signicant for the successful advancement of chemical component research and Q-marker determination. 29,30 Currently, chromatography analysis, as a more conventional and effective identication method of traditional medicines, commonly includes paper chromatography, thin-layer chromatography, gas chromatography (GC), and high-performance liquid chromatography (HPLC) in actual identication. [31][32][33] NMR and MS play crucial roles in the phases of component identication from novel chemical structures to Q-marker discovery in a high-throughput environment.…”

Qualitative and quantitative analysis of Porana sinensis Hemsl by UHPLC‐Q‐Exactive MS, TLC autographic method and DART‐MS

“…TCM is a multi‐component, multi‐target agent that treats complex diseases in a holistic way . Therefore, it is essential to evaluate the chemical composition of each TCM . In this study, a Q‐Exactive Focus tandem mass spectrometry (MS/MS) method was used to obtain high‐resolution mass spectra of the various components in P. sinensis .…”

“…[6][7][8] Therefore, it is essential to evaluate the chemical composition of each TCM. 9 In this study, a Q-Exactive Focus tandem mass spectrometry (MS/MS) method was used to obtain highresolution mass spectra of the various components in P. sinensis. Total alkaloid contents Baogongteng A and Baogongteng C in Erycibe plant species are toxic substances with cholinergic functions.…”