2021
DOI: 10.1371/journal.pgen.1009240
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Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior

Abstract: Examining the role of chromatin modifications and gene expression in neurons is critical for understanding how the potential for behaviors are established and maintained. We investigate this question by examiningDrosophila melanogaster fru P1neurons that underlie reproductive behaviors in both sexes. We developed a method to purify cell-type-specific chromatin (Chromatag), using a tagged histone H2B variant that is expressed using the versatile Gal4/UAS gene expression system. Here, we use Chromatag to evaluat… Show more

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Cited by 11 publications
(32 citation statements)
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References 104 publications
(193 reference statements)
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“…There are 147 genes that are either male- or female-biased, depending on the cluster ( Supplemental Table 9 ). When we compare these sex-biased genes to genes identified as sex-biased in our previous cell-type-specific TRAP study at 48hrAPF, we find significant overlap between gene lists ( Supplemental Table 4 ) (Palmateer et al ., 2021).…”
Section: Resultsmentioning
confidence: 89%
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“…There are 147 genes that are either male- or female-biased, depending on the cluster ( Supplemental Table 9 ). When we compare these sex-biased genes to genes identified as sex-biased in our previous cell-type-specific TRAP study at 48hrAPF, we find significant overlap between gene lists ( Supplemental Table 4 ) (Palmateer et al ., 2021).…”
Section: Resultsmentioning
confidence: 89%
“…In total, we find 3,582 marker genes in the male data set, 3,203 in the female data set, and 3,662 in the full data set ( Supplemental Table 4 ). When we determine the overlap of the marker genes in the three analyses, the majority are shared (2,573 genes; Figure 1C ), indicating that fru P1 neurons from males and females have overlapping gene expression profiles ( Figure 1 ), as we previously showed (Newell et al ., 2016; Palmateer et al ., 2021). However, each analysis reveals unique marker genes, with the most occurring in the male data set ( Figure 1C ).…”
Section: Resultsmentioning
confidence: 99%
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“…Visualization of reads density and the genomic environment is carried out with the JBROWSE genome browser [ 65 ]. RNA-Seq datasets (SRA reference PRJNA644503; https://www.ncbi.nlm.nih.gov/bioproject/PRJNA644503 ) from wild type pupae are from [ 66 ] and PRO-seq data (GEO reference GSE131160; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE131160 ) are from Ueberschar et al [ 37 ].…”
Section: Methodsmentioning
confidence: 99%