Analysis of Cell-Seeded 3-Dimensional Bone Constructs Manufactured In Vitro With Hydroxyapatite Granules Obtained From Red Algae

Turhani, Dritan; Watzinger, Elisabeth; Weiβenböck, Martina; Cvikl, Barbara; Thurnher, Dietmar; Wittwer, Gert; Yerit, Kaan; Ewers, Rolf

doi:10.1016/j.joms.2005.01.006

Cited by 36 publications

(17 citation statements)

References 37 publications

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“…These findings support work by Turhani and Weiβenböckwho also investigated the cell response of a different QUB HA to BioOss ® [46]. The main differences between QUB HA and Bio-Oss ® were pore size, granule shape and S BET .…”

Section: Citationsupporting

confidence: 88%

An in vitro Study to Assess the Potential of a Unique Micro porous Algal Derived Cap Bone Void Filler in Comparison with Clinically-Used Bone Void Fillers

Walsh¹

2011

J Tissue Sci Eng

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Section: Citationsupporting

confidence: 88%

An in vitro Study to Assess the Potential of a Unique Micro porous Algal Derived Cap Bone Void Filler in Comparison with Clinically-Used Bone Void Fillers

Walsh¹

2011

J Tissue Sci Eng

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“…This material has been proven to have biocompatible properties as a bone graft substitute in vivo [Ewers and Schumann, 1994;Schopper et al, 2003;Ewers et al, 2004]. In vitro studies showed that this material supports the proliferation and differentiation of human osteoblast-like cells on its surface when grown in monolayer [Turhani et al, 2003[Turhani et al, , 2005a and in cellseeded three-dimensional bone composites [Turhani et al, 2005b, d].…”

mentioning

confidence: 99%

Particle Size of Hydroxyapatite Granules Calcified from Red Algae Affects the Osteogenic Potential of Human Mesenchymal Stem Cells in vitro

Weissenboeck

Stein

Undt

et al. 2006

Cells Tissues Organs

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Hydroxyapatite (HA) microparticles as a carrier in an injectable tissue-engineered bone filler are considered promising candidates for the treatment of small bone defects in the craniomaxillofacial region. HA granules calcified from red algae, varying in size, were evaluated in vitro for their suitability to be used as a carrier for human mesenchymal stem cells (hMSCs). Three groups of granules were produced in grain sizes of 10–100, 200–500 and 600–1,000 µm. After seeding and culturing hMSCs under osteogenic differentiation conditions onto HA particles for 3, 6 and 9 days, cellular proliferation (tetrazolium salt, XTT), alkaline phosphatase (ALP)-specific activity and total protein synthesis were investigated. The osteoblastic phenotype of the cells was evaluated by assaying the bone-specific genes osteocalcin, osteopontin and collagen type I. XTT assay revealed significantly higher (p < 0.01) proliferation of cells grown on the smallest grain size after 9 days of culture. Regarding ALP-specific activity, significantly higher levels of activity were detected in cells grown on the smallest grain size. Different grain sizes had no significant effects on the secretion of osteocalcin and osteopontin. Collagen type I production was significantly higher (p < 0.05) in cells grown on the biggest grain size in comparison with the two other grain sizes. These results show that the particle size of HA microparticles affects the osteogenic potential of cultured hMSCs and lead to the conclusion that particle size has differential effects on ALP-specific activity and collagen type I production.

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“…All these alternatives have specific limitations such as restricted amount of tissue obtained with autogenous grafts, difficulty of preparing grafts with the desired shape, and risk of transferring pathogens when using synthetic implants and allograft 1 . The regenerative medicine that makes use of stem cells is a fast developing field in the treatment of bone defects 2 .…”

Section: Introductionmentioning

confidence: 99%

Use of calcium phosphate cement scaffolds for bone tissue engineering: in vitro study

et al. 2011

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Purpose: To evaluate the ability of macroporous tricalcium phosphate cement (CPC) scaffolds to enable the adhesion, proliferation, and differentiation of mesenchymal stem cells derived from human bone marrow. Methods: Cells from the iliac crest of an adult human donor were processed and cultured on macroporous CPC discs. Paraffin spheres sized between 100 and 250µm were used as porogens. Cells were cultured for 5, 10, and 15 days. Next, we assessed cells' behavior and morphology on the biomaterial by scanning electron microscopy. The expression levels of the BGLA and SSP1 genes and the alkaline phosphatase (ALP) activity were quantified by the quantitative real-time polymerase chain reaction technique (QT-PCR) using the fluorophore SYBR GREEN®. Results: QT-PCR detected the expression of the BGLA and SSP1 genes and the ALP activity in the periods of 10 and 15 days of culture. Thus, we found out that there was cell proliferation and differentiation in osteogenic cells. Conclusion: Macroporous CPC, with pore sized between 100 and 250µm and developed using paraffin spheres, enables adhesion, proliferation, and differentiation of mesenchymal stem cells in osteogenic cells and can be used as a scaffold for bone tissue engineering.

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Analysis of Cell-Seeded 3-Dimensional Bone Constructs Manufactured In Vitro With Hydroxyapatite Granules Obtained From Red Algae

Cited by 36 publications

References 37 publications

An in vitro Study to Assess the Potential of a Unique Micro porous Algal Derived Cap Bone Void Filler in Comparison with Clinically-Used Bone Void Fillers

An in vitro Study to Assess the Potential of a Unique Micro porous Algal Derived Cap Bone Void Filler in Comparison with Clinically-Used Bone Void Fillers

Particle Size of Hydroxyapatite Granules Calcified from Red Algae Affects the Osteogenic Potential of Human Mesenchymal Stem Cells in vitro

Use of calcium phosphate cement scaffolds for bone tissue engineering: in vitro study

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