Analysis of cell–nanoparticle interactions and imaging of <i>in vitro</i> labeled cells showing barcorded endosomes using fluorescent thiol-organosilica nanoparticles surface-functionalized with polyethyleneimine

Nakamura, Michihiro; Nakamura, Junna; Mochizuki, Chihiro; Kuroda, Chika; Kato, Shigekí; Haruta, Tomohiro; Kakefuda, Mayu; Sato, Shun; Tamanoi, Fuyuhiko; Sugino, Norihiro

doi:10.1039/d1na00839k

Cited by 4 publications

(12 citation statements)

References 82 publications

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“…Previously, we reported PEI-resistant J774A.1 cells that could not uptake OS/ F-PEI particles very well compared with particles of similar size without PEI. 25 These results indicated that the uptake of J774A.1 cells is controlled not only by surface positive charge but also by particle size and surface structure, and even by a combination of these factors, indicating the uptake selectivity of 774A.1 cells.…”

Section: Time-lapse Microscopic Imaging and Single-cell Analysis Of P...mentioning

confidence: 72%

“…Previously, we reported that the hydrodynamic diameters of surface-functionalized thiol-organosilica particles (diameter 151 nm) with various molecular weights of PEI showed various increases, but no decrease. 25 Because the diameters of OS/F400PEI and OS/F680PEI did not decrease on STEM (Figure 1 and Table 1), PEI surface functionalization would hasten Brownian motion of particles of OS/F400 and OS/F680, but not OS/F150. PEI surface functionalization could change the structure and interaction with water molecules in a particle-size-dependent manner.…”

Section: Preparation and Characterization Of Fluorescentmentioning

confidence: 94%

“…Previously, we reported that the hydrodynamic diameter and PDI of PEI surface-functionalized thiol-organosilica particles also varied with the PEI's molecular weight. 25 Further studies on molecule−surface residue interactions in particles of various particle sizes and PEI molecular weights are required to explain these variations in the hydrodynamic diameter and PDI by PEI.…”

Section: Preparation and Characterization Of Fluorescentmentioning

confidence: 99%

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Size and Surface Properties of Functionalized Organosilica Particles Impact Cell–Particle Interactions Including Mitochondrial Activity

Nakamura,

Shiohama,

Röth

et al. 2024

ACS Appl. Mater. Interfaces

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Section: Time-lapse Microscopic Imaging and Single-cell Analysis Of P...mentioning

confidence: 72%

Section: Preparation and Characterization Of Fluorescentmentioning

confidence: 94%

Section: Preparation and Characterization Of Fluorescentmentioning

confidence: 99%

See 1 more Smart Citation

Size and Surface Properties of Functionalized Organosilica Particles Impact Cell–Particle Interactions Including Mitochondrial Activity

Nakamura,

Shiohama,

Röth

et al. 2024

ACS Appl. Mater. Interfaces

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“…A dose-dependent increase in the fluorescence intensity of the particles was observed, which was used as a reference to determine the exposure time for the fluorescence microscopy analysis of lung tissue. The fluorescence stability of these particles was previously evaluated, , which showed no predominant changes in particle fluorescence intensity after 24 h of incubation under acidic conditions, indicating good stability in cells after uptake.…”

Section: Resultsmentioning

confidence: 99%

Cellular Distribution and Intracellular Localization of Different Sizes of Fluorescent Thiol-Organosilica Particles in Mouse Lungs

Shiohama,

Nakamura,

Nakamura

2024

ACS Appl. Mater. Interfaces

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We investigated the distribution of intratracheally administered thiolorganosilica (thiol-OS) particles in mouse lungs. Toward this end, single doses of thiol-OS particles containing fluorescein (140 nm in diameter) (F140) and rhodamine B (Rh) (Rh160, Rh280, Rh420, Rh640, and Rh1630 with diameters of 160, 280, 420, 640, and 1630 nm, respectively) were administered. After 24 h, fluorescence imaging revealed homogeneous fluorescence with a patchier pattern on the lung surface and no difference among the six particle sizes. Simultaneous dual administration of Rh and F140 particles did not reveal any size-dependent differences in the lung surface fluorescence. Fluorescence microscopy of the lung sections revealed a similar tissue distribution in the fluorescent areas of Rhs and F140. Some fluorescent areas showed one type of particle fluorescence or only one fluorescence. Cellular distribution of particles was observed in bronchoalveolar lavage cells and lung sections under a high magnification, and correlative light and electron microscopy revealed large cells with fluorescence corresponding to both particle types and small cells with fluorescence of individual particle types, indicating a cell-subset-dependent particle size effect. Rh280, Rh420, and Rh640 exhibited significant size effects and were taken up by alveolar macrophages. Extracellular particles were observed, indicating that saturation exceeded the particle dose threshold in the alveoli. F140 taken up by small and large macrophages colocalized with CD68, CD11c, and CD11b and correlated with CD11c. The size effect, intracellular localization, and extracellular distribution of particles provide insights into lung and systemic drug delivery.

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“…This result, in which AC (−)/thiol-OS/Au/PEI and AC300/thiol-OS/Au/PEI show an increase in the cell death ratio on DAY 4 without X-ray irradiation by cell counts and cell viability counts, also shows the same tendency as the results of cell counts. The slight decrease in cell viability for the 4T1 cells treated with AC (−)/thiol-OS/Au/PEI and AC300/thiol-OS/Au/PEI implies the cytotoxicity of the modified-PEI on NPs because AC (−)/thiol-OS/Au/PEI and AC300/thiol-OS/Au/PEI are absorbed more than AC600/thiol-OS/Au/PEI and AC800/thiol-OS/Au/PEI (Table ), indicating that the cytotoxicity of PEI , accelerated the PEI-modified NP uptake.…”

Section: Results and Discussionmentioning

confidence: 99%

Effects of Au States in Thiol-Organosilica Nanoparticles on Enzyme-like Activity for X-ray Sensitizer Application: Focus on Reactive Oxygen Species Generation in Radiotherapy

Mochizuki

Nakamura

2023

ACS Omega

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In radiotherapy, the use of Au nanoparticles (Au NPs) has been proposed to enhance cell damage by X-ray irradiation. Although the role of Au in radiotherapy is not fully understood, the catalytic activity of Au has been actively studied in the industrial field. Moreover, owing to their enzyme-like activity and high biocompatibility in vitro and in vivo, Au NPs present significant potential for biological applications. In this study, we incorporated different Au states both on the surface and embedded in thiol-organosilica (thiol-OS/Au series) to investigate the efficiency of anticancer cell activity of Au in radiotherapy. The thiol-OS/Au series comprised different Au(I)/Au(0) ratios and Au NPs, and different sizes of Au NPs were embedded in thiol-OS/Au. These thiol-OS/Au series samples were evaluated for enzyme-like activities in reactive oxygen species (ROS) generation by X-ray irradiation. Thiol-OS/Au embedded with small Au NPs (AC600/thiol-OS/Au) exhibited peroxidase (POD)-like activity under acidic conditions. This POD-like activity improved ROS generation and cytotoxicity under X-ray irradiation. Furthermore, AC600/thiol-OS/Au exhibited catalase (CAT)-like activity under basic conditions and showed no cytotoxicity toward nonirradiated cells. These results revealed the efficiency of functionalizing with small Au NPs that possess pH-controlled POD-and CAT-like activity as a radiosensitizer. We compared the suitability of using Au with different states to obtain the thiol-OS/Au series samples for application as radiosensitizers. The findings of this study will aid the design of efficacious strategies for the Au nanostructure-based radiotherapy of cancer cells.

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Analysis of cell–nanoparticle interactions and imaging of in vitro labeled cells showing barcorded endosomes using fluorescent thiol-organosilica nanoparticles surface-functionalized with polyethyleneimine

Abstract: Biomedical imaging using cell labeling is an important technique to visualize cell dynamics in the body. To label cells thiol-organosilica nanoparticles (thiol-OS) containing fluorescein (thiol-OS/Flu) and containing rhodamine B (thiol-OS/Rho)...

Cited by 4 publications

References 82 publications

Size and Surface Properties of Functionalized Organosilica Particles Impact Cell–Particle Interactions Including Mitochondrial Activity

Size and Surface Properties of Functionalized Organosilica Particles Impact Cell–Particle Interactions Including Mitochondrial Activity

Cellular Distribution and Intracellular Localization of Different Sizes of Fluorescent Thiol-Organosilica Particles in Mouse Lungs

Effects of Au States in Thiol-Organosilica Nanoparticles on Enzyme-like Activity for X-ray Sensitizer Application: Focus on Reactive Oxygen Species Generation in Radiotherapy

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