Analysis of Cell Growth in Three-Dimensional Scaffolds

Dunn, James C.Y.; Chan, Wan Yin; Cristini, Vittorio; Kim, Junseok; Lowengrub, John; Singh, Shivani; Wu, Benjamin M.

doi:10.1089/ten.2006.12.705

Cited by 99 publications

(90 citation statements)

References 18 publications

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“…Prompted by this prospect, recent years have seen a rapid increase in the number of mathematical models aimed at the regeneration of articular cartilage [9,11,14,22,33,34]; overview in [41]. Mathematical modelling in tissue engineering broadly divides into structural and nutrient approaches, and few authors have considered the interaction between the two.…”

Section: Introductionmentioning

confidence: 99%

“…Models for metabolic networks are being developed to support fundamental biological research [10]. However, due to lack of available data, most models that couple cellular uptake to transport in cartilage tissue engineering adopt a far simpler, workable, approach by considering only a single solute assumed to be critical [9,11,22,33,34], or alternatively, couple glucose, oxygen and lactate using a simple mass balance [42]. On the other hand, in related tissues such as the intervertebral disk, models have been based on actual, empirically measured, coupled relationships [31].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Design criteria for a printed tissue engineering construct: A mathematical homogenization approach

Shipley¹,

Jones²,

Dyson

et al. 2009

Journal of Theoretical Biology

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Cartilage tissue repair procedures currently under development aim to create a construct in which patient-derived cells are seeded and expanded ex vivo before implantation back into the body. The key challenge is producing physiologically realistic constructs that mimic real tissue structure and function. One option with vast potential is to print strands of material in a 3D structure called a scaffold that imitates the real tissue structure; the strands are composed of gel seeded with cells and so provide a template for cartilaginous tissue growth. The scaffold is placed in the construct and pumped with nutrient-rich culture medium to supply nutrients to the cells and remove waste products, thus promoting tissue growth.In this paper we use asymptotic homogenization to determine the effective flow and transport properties of such a printed scaffold system. These properties are used to predict the distribution of nutrient/waste products through the construct, and to specify design criteria for the scaffold that will optimise the growth of functional tissue. Keywords

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Design criteria for a printed tissue engineering construct: A mathematical homogenization approach

Shipley¹,

Jones²,

Dyson

et al. 2009

Journal of Theoretical Biology

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“…Typically applied static, i.e. non-agitated, culture conditions generally limit inoculation and proliferation efficiency due to obvious limitation of mass transfer inside biomaterial constructs (Dunn et al, 2006). Each bioprocess development improving cell proliferation in the threedimensional inner structure of scaffolds is strongly invited to overcome these limitations.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation

Pilarek

Godlewska

Kuźmińska

et al. 2017

Chemical and Process Engineering

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One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously waveinduced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.

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“…Collective motion has been treated in macroscopic continuum models. Usually these models lead to a set of partial differential equations for the masses or concentrations of cells and chemicals (62)(63)(64). Normally the mass balance of cells is governed by a non-linear reaction-diffusion equation and the mass balance of chemicals by a diffusion equation.…”

Section: Introductionmentioning

confidence: 99%

One-Dimensional Migration of Olfactory Ensheathing Cells on Synthetic Materials: Experimental and Numerical Characterization

Pérez-Garnés

Martínez‐Ramos

Barcia

et al. 2012

Cell Biochem Biophys

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Olfactory ensheathing cells (OECs) are of great interest for regenerative purposes since they are believed to aid axonal growth. With the view set on strategies to achieve reconnection between neuronal structures it is of greatest importance to characterize the behaviour of these cells on long thread-like structures that may efficiently guide cell spread in a targeted way. Here rat OECs were studied on polycaprolactone long monofilaments, on long bars and on discs. Polycaprolactone turns out to be an excellent substrate for OECs. The cells cover long distances along the monofilaments, and colonize completely these structures. With the help of a one-dimensional analytical model a migration coefficient, a net proliferation rate constant, and the fraction of all cells which undergo migration were obtained. The separate effect of the three phenomena summarized by these parameters on the colonization patterns of the onedimensional paths was qualitatively discussed. Other features of interest were also determined, such as the speed of the advance front of colonization and the order of the kinetics of net cell proliferation. Characterizing migration by means of these quantities may be useful for comparing and predicting features of the colonization process (such as times, patterns, advance fronts, proportion of motile cells) of different cell-substrate combinations.

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Analysis of Cell Growth in Three-Dimensional Scaffolds

Cited by 99 publications

References 18 publications

Design criteria for a printed tissue engineering construct: A mathematical homogenization approach

Design criteria for a printed tissue engineering construct: A mathematical homogenization approach

Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation

One-Dimensional Migration of Olfactory Ensheathing Cells on Synthetic Materials: Experimental and Numerical Characterization

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