Analysis of ceftazidime and related compounds by micellar electrokinetic chromatography

Porrà, R; Farina, A.; Cotichini, V.; Lecce, Raffaele

doi:10.1016/s0731-7085(98)00178-2

Cited by 17 publications

(5 citation statements)

References 6 publications

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“…In the latter case, SDS above its micellar concentration is often used, which permits analysis of neutral compounds together with charged compounds . MEKC was employed for analysis of various caphalosporins , including cefepime and ceftazidime . When asked to provide TDM service for cefepime, our laboratory evaluated the reported MEKC assay conditions with direct sample injection .…”

Section: Resultsmentioning

confidence: 99%

An MEKC assay for the therapeutic drug monitoring of cefepime

Theurillat

Sendi

Thormann

2013

J of Separation Science

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The development of a robust assay based on MEKC for cefepime in human serum and plasma with internal quality assurance is reported. Sample preparation comprises protein precipitation in the presence of SDS at pH 4.5. This is a gentle approach for which decomposition of cefepime during sample handling is negligible. After hydrodynamic sample injection of the supernatant, analysis occurs in a phosphate/borate buffer at pH 9.1 with 75 mM SDS using normal polarity and analyte detection at 257 nm. The MEKC run time interval and throughput are about 5 min and seven samples per hour, respectively. The calibration range for cefepime is 1-60 μg/mL, with 1 μg/mL being the LOQ. The performance of the assay with multilevel internal calibration was assessed with calibration and control samples. The assay is shown to be simple, inexpensive, reproducible, and robust. It was applied to determine cefepime levels in the sera of critically ill patients and to assess the instability of cefepime in patient and control samples. Our data revealed that serum containing cefepime can be stored at -20°C for a short time, whereas for long-term storage, samples have to be kept at -70°C.

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Section: Resultsmentioning

confidence: 99%

An MEKC assay for the therapeutic drug monitoring of cefepime

Theurillat

Sendi

Thormann

2013

J of Separation Science

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“…Since 1974, many studies were published on the electrophoretic behavior of sulfonamides [27][28][29]. A validated CE method for the identification and separation of the b-lactame antibiotic ceftazidime in the presence of its major impurity, that is, pyridine, was reported [30]. CE methods can also be used to separate impurities from, for example, central nervous system drugs [31], antiinflammatory drugs [32], cardiovascular drugs [33], and antitumoral drugs [34].…”

Section: Cementioning

confidence: 99%

Analytical Techniques and Quality Control of Pharmaceuticals

Manna¹,

Rossi²,

Fioravanti³

2012

Analytical Techniques for Clinical Chemistry

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“…The method was applied to analyze three bulk samples of ceftazidime where the levels of pyridine were less than 0.15% [57].…”

Section: Chemotherapeutic Agentsmentioning

confidence: 99%

Impurity analysis of pharmaceuticals using capillary electromigration methods

Jouyban

Kenndler

2008

Electrophoresis

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This review deals with the determination of impurities in pharmaceuticals by electromigration methods in the capillary format. These separation methods are either based on the different effective mobility of the charged analytes (as in zone electrophoresis and isotachophoresis) or include hybrid methods such as micellar electrokinetic chromatography, microemulsion electrokinetic chromatography and electrochromatography. The pharmaceutically active compounds under consideration belong to chemotherapeutic agents, central nervous system drugs, histamine receptor drugs, cardiovascular drugs, anticancer drugs, anti-inflammatory drugs and some other drugs. The review discusses about 150 publications from the period between 1980 and 2007 with special emphasis on the recent trends and gives details about the experimental conditions applied for analyses and the obtained analytical performance parameters.

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Analysis of ceftazidime and related compounds by micellar electrokinetic chromatography

Cited by 17 publications

References 6 publications

An MEKC assay for the therapeutic drug monitoring of cefepime

An MEKC assay for the therapeutic drug monitoring of cefepime

Analytical Techniques and Quality Control of Pharmaceuticals

Impurity analysis of pharmaceuticals using capillary electromigration methods

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