Analysis of cDNA transcripts from Coniothyrium minitans reveals a diverse array of genes involved in key processes during sclerotial mycoparasitism

Muthumeenakshi, S.; Sreenivasaprasad, S.; Rogers, Christopher W.; Challen, Michael P.; Whipps, J. M.

doi:10.1016/j.fgb.2007.07.011

Cited by 30 publications

(29 citation statements)

References 70 publications

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“…This study highlighted the importance of cell-cell adhesion and hyphal bridge formation during vegetative incompatibility in A. areolatum and it is similar to that observed in other fungi (Pandey et al, 2004;Muthumeenakshi et al, 2007). Various ESTs were identified that encode putative proteins involved in the generation and breakdown of cell walls and in the transport of components necessary for these processes (Glass et al, 2000;Pandey et al, 2004;Muthumeenakshi et al, 2007).…”

Section: Discussionsupporting

confidence: 72%

“…Various ESTs were identified that encode putative proteins involved in the generation and breakdown of cell walls and in the transport of components necessary for these processes (Glass et al, 2000;Pandey et al, 2004;Muthumeenakshi et al, 2007). Cell-cell adhesion and recognition in A. areolatum further appear to be lectin-mediated, because the two glycoprotein-encoding ESTs identified in this study encode a putative mucin and a mannoprotein.…”

Section: Discussionmentioning

confidence: 77%

“…Another approach has been to study differentially expressed genes that are specifically up-regulated during vegetative incompatibility, which led to the discovery of the induced during incompatibilty (idi) genes in P. anserina (Bourges et al, 1998). More recently, PCR-based suppression subtractive hybridization (SSH) has been used to study the genes involved in fungalfungal interactions (Carpenter et al, 2005;Muthumeenakshi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Gene expression associated with vegetative incompatibility in Amylostereum areolatum

Nest¹,

Steenkamp²,

Slippers³

et al. 2011

Fungal Genetics and Biology

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In filamentous fungi, vegetative compatibility among individuals of the same species is determined by the genes encoded at the heterokaryon incompatibility (het) loci. The hyphae of genetically similar individuals that share the same allelic specificities at their het loci are able to fuse and intermingle, while different allelic specificities at the het loci result in cell death of the interacting hyphae. In this study, suppression subtractive hybridization (SSH) followed by pyrosequencing and quantitative reverse transcription PCR were used to identify genes that are selectively expressed when vegetatively incompatible individuals of Amylostereum areolatum interact. The SSH library contained genes associated with various cellular processes, including cell-cell adhesion, stress and defence responses, as well as cell death. Some of the transcripts encoded proteins that were previously implicated in the stress and defence responses associated with vegetative incompatibility. Other transcripts encoded proteins known to be associated with programmed cell death, but have not previously been linked with vegetative incompatibility.Results of this study have considerably increased our knowledge of the processes underlying vegetative incompatibility in Basidiomycetes in general and A. areolatum in particular.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gene expression associated with vegetative incompatibility in Amylostereum areolatum

Nest¹,

Steenkamp²,

Slippers³

et al. 2011

Fungal Genetics and Biology

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“…For example, A. aculeatus Asp-4 up-regulated numerous genes and proteins involved in mitigation of environmental stress and dissolution of sclerotial compounds during colonization and degradation of sclerotia of S. sclerotiorum [81]. Omic studies of other biological control interactions revealed equally complex gene and/or protein expression profiles by biological control agents during interactions with pathogens or the environment [82][83][84]. In turn, soils and plant surfaces, where biological control agents are expected to effect disease control, are highly heterogeneous regarding compounds and conditions that impact expression of genes important to biological control; ultimately impacting expression of these genes and reliability and efficacy of disease control (reviewed in [80]).…”

Section: Use Of Plant-beneficial Microbes For Sustainable Crop Producmentioning

confidence: 99%

Sustainable Agriculture—Enhancing Environmental Benefits, Food Nutritional Quality and Building Crop Resilience to Abiotic and Biotic Stresses

Roberts¹,

Mattoo²

2018

Agriculture

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Feeding nutrition-dense food to future world populations presents agriculture with enormous challenges as estimates indicate that crop production must as much as double. Crop production cannot be increased to meet this challenge simply by increasing land acreage or using past agricultural intensification methods. Food production doubled in the past through substantial use of synthetic fertilizer, pesticides, and irrigation, all at significant environmental cost. Future production of nutrition-dense food will require next-generation crop production systems with decreased reliance on synthetic fertilizer and pesticide. Here, we present three case studies detailing the development of cover crops and plant-beneficial microbes for sustainable, next-generation small grain, tomato, and oilseed rape production systems. Cover crops imparted weed and pathogen control and decreased soil erosion and loss of soil nitrogen, phosphorus and carbon, while plant-beneficial microbes provided disease control and phosphorus fertility. However, yield in these next-generation crop production systems at best approximated that associated with current production systems. We argue here that to substantially increase agricultural productivity, new crop germplasm needs to be developed with enhanced nutritional content and enhanced tolerance to abiotic and biotic stress. This will require using all available technologies, including intensified genetic engineering tools, in the next-generation cropping systems.

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“…The cmg1 exo-b-1,3-glucanase gene was shown to be upregulated when the fungus was grown on media containing sclerotia as the sole carbon source (Giczey et al, 2001). Recently we have identified 251 unique transcripts of C. minitans upregulated during growth on sclerotia, and representing genes involved in diverse processes including signalling, detoxification and stress responses (Muthumeenakshi et al, 2007). The development of efficient and reliable transformation and insertional mutagenesis systems for C. minitans, both protoplastmediated and Agrobacterium-mediated (ATMT), are enabling functional characterization of key genes (Jones et al, 1999;Rogers et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Disruption of the Coniothyrium minitans PIF1 DNA helicase gene impairs growth and capacity for sclerotial mycoparasitism

et al. 2008

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A non-mycoparasitic restriction enzyme-mediated DNA integration (REMI) mutant of Coniothyrium minitans (R2427) contains two tandem plasmid copies integrated towards the 39 end of an ORF. The predicted polypeptide (845 aa) exhibits high similarity with DNA-helicase proteins from other filamentous fungi and yeasts that play a role in mitochondrial DNA maintenance and repair. Disruption of the C. minitans PIF1 DNA helicase gene results in altered morphology, reduced growth rates and a concomitant loss in ability to mycoparasitize sclerotia of Sclerotinia sclerotiorum. In infection bioassays, R2427 exhibited sparse mycelial growth on the surface of live sclerotia, but no mycelia were detected inside the sclerotia. Conversely, R2427 readily colonized autoclaved sclerotia. Complementation of the mutant with wild-type PIF1 restored normal mycelial growth and mycoparasitic capability, confirming a functional role in the host-pathogen interaction. The C. minitans PIF1 DNA helicase may maintain mitochondrial stability in response to reactive oxygen species, either produced endogenously within the mycoparasite, or exogenously from the sclerotial host.

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Analysis of cDNA transcripts from Coniothyrium minitans reveals a diverse array of genes involved in key processes during sclerotial mycoparasitism

Cited by 30 publications

References 70 publications

Gene expression associated with vegetative incompatibility in Amylostereum areolatum

Gene expression associated with vegetative incompatibility in Amylostereum areolatum

Sustainable Agriculture—Enhancing Environmental Benefits, Food Nutritional Quality and Building Crop Resilience to Abiotic and Biotic Stresses

Disruption of the Coniothyrium minitans PIF1 DNA helicase gene impairs growth and capacity for sclerotial mycoparasitism

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