Analysis of Campylobacter spp. contamination in broilers from the farm to the final meat cuts by using restriction fragment length polymorphism of the polymerase chain reaction products

Takahashi, Ryouta; Shahada, Francis; Chuma, Takehisa; Okamoto, Karoku

doi:10.1016/j.ijfoodmicro.2006.04.043

Cited by 33 publications

(23 citation statements)

References 21 publications

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“…In addition, the application of bacterial pathogen typing (Ertas et al 2004;Takahashi et al 2006;Wilson et al 2008;Hannon et al 2009;Huang et al 2009;Sheppard et al 2009) and direct detection of host-species specific bacterial DNA (Bernhard & Field 2000b;Dick et al 2005a) could be useful in identifying sources (microbial source tracking; MST) of faecal contamination in surface waters. While library-and culture-independent MST methods are inexpensive, quick, and easy to use (Bernhard & Field 2000b), molecular subtyping methods for pathogens may provide confirmation, and in some cases greater discrimination in the identification of faecal sources of contamination.…”

Section: Introductionmentioning

confidence: 99%

The occurrence and sources of Campylobacter spp., Salmonellaenterica and Escherichia coli O157:H7 in the Salmon River, British Columbia, Canada

Jokinen

Schreier

Mauro

et al. 2009

Journal of Water and Health

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In this study, we wished to assess the prevalence and determine the sources of three zoonotic bacterial pathogens (Salmonella, Campylobacter, and Escherichia coli O157:H7) in the Salmon River watershed in southwestern British Columbia. Surface water, sewage, and animal faecal samples were collected from the watershed. Selective bacterial culture and PCR techniques were used to isolate these three pathogens and indicator bacteria from these samples and characterize them. Campylobacter was the most prevalent pathogen in all samples, followed by Salmonella, and E. coli O157:H7. E. coli O157:H7 and Salmonella isolation rates from water, as well as faecal coliform densities correlated positively with precipitation, while Campylobacter isolation rates correlated negatively with precipitation. Analysis of DNA extracted from water samples for the presence of Bacteroides host-species markers, and comparisons of C. jejuni flaA-RFLP types and Salmonella serovars from faecal and water samples provided evidence that human sewage and specific domestic and wild animal species were sources of these pathogens; however, in most cases the source could not be determined or more than one source was possible. The frequent isolation of these zoonotic pathogens in the Salmon River highlights the risks to human health associated with intentional and unintentional consumption of untreated surface waters.

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Section: Introductionmentioning

confidence: 99%

The occurrence and sources of Campylobacter spp., Salmonellaenterica and Escherichia coli O157:H7 in the Salmon River, British Columbia, Canada

Jokinen

Schreier

Mauro

et al. 2009

Journal of Water and Health

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“…The Campylobacter flaA gene was selected because it is known to be highly variable (28) and has been used in several genotyping schemes (35,48). Genomic DNA was isolated from all campylobacters using a NucleoSpin blood kit (Macherey-Nagel, Duren, Germany).…”

Section: Methodsmentioning

confidence: 99%

Development of a Strain-Specific Molecular Method for Quantitating Individual Campylobacter Strains in Mixed Populations

Elvers

Helps

Wassenaar

et al. 2008

Appl Environ Microbiol

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The identification of sites resulting in cross-contamination of poultry flocks in the abattoir and determination of the survival and persistence of campylobacters at these sites are essential for the development of intervention strategies aimed at reducing the microbial burden on poultry at retail. A novel molecule-based method, using strain-and genus-specific oligonucleotide probes, was developed to detect and enumerate specific campylobacter strains in mixed populations. Strain-specific oligonucleotide probes were designed for the short variable regions (SVR) of the flaA gene in individual Campylobacter jejuni strains. A 16S rRNA Campylobacter genus-specific probe was also used. Both types of probes were used to investigate populations of campylobacters by colony lift hybridization. The specificity and proof of principle of the method were tested using strains with closely related SVR sequences and mixtures of these strains. Colony lifts of campylobacters were hybridized sequentially with up to two labeled strain-specific probes, followed by the generic 16S rRNA probe. SVR probes were highly specific, differentiating down to 1 nucleotide in the target sequence, and were sufficiently sensitive to detect colonies of a single strain in a mixed population. The 16S rRNA probe detected all Campylobacter spp. tested but not closely related species, such as Arcobacter skirrowi and Helicobacter pullorum. Preliminary field studies demonstrated the application of this technique to target strains isolated from poultry transport crate wash tank water. This method is quantitative, sensitive, and highly specific and allows the identification and enumeration of selected strains among all of the campylobacters in environmental samples.

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“…Food processing areas that constitute critical control points in poultry processing plants are usually scalding, defeathering and evisceration, since the carcass contamination occurs there by leakage of the contaminated faeces from the cloaca and visceral rupture of the ceca carrying a high Campylobacter load (Berrang et al, 2001;Stern & Robach, 2003;Takahashi et al, 2006;Boysen & Rosenquist, 2009;Silva et al, 2011). Automated defeathering represents a high risk practice since cloacal contents can cause contamination of the carcasses (Berrang et al, 2001).…”

Section: Broiler Slaughterhouses -Carcassesmentioning

confidence: 99%

Campylobacter spp. infection in humans and poultry

Natsos

Koutoulis

Sossidou

et al. 2018

J Hellenic Vet Med Soc

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Campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide. The infection may be subclinical or cause disease of variable severity. The eating and handling of improperly cooked or raw broiler meat has been shown to be one of the most important sources of human campylobacteriosis. Birds carrying Campylobacter are asymptomatic colonizers without any clinical signs. Broilers are considered Campylobacter free after hatching and become colonized by exposure to viable bacteria from the environment. Several risk factors can result in the introduction of Campylobacter into the flocks making it difficult to keep chicken flocks free of Campylobacter throughout the rearing period. Lack of biosecurity measures, season, age, partial depopulation practices, flock size, type of production system, presence of other animals on farm, water quality, presence of rodents and mechanical transmission via insects are considered to be some of the risk factors associated with horizontal transmission. The control of Campylobacter in poultry seems crucial for the reduction of human campylobacteriosis cases. In Greece, there has been a dearth of information on prevalence and risk factors of Campylobacter in broiler flocks. Therefore, it is essential to initially investigate the prevalence of Campylobacter infection on farms and in poultry carcasses and subsequently the risk factors at all production stages of broiler meat and plan intervention studies to help reducing the disease in humans. This paper review the most recent data reported worldwide on Campylobacter infection in humans and poultry in order to provide an overview of trends, risks, possible causes and mechanisms of transmission routes.

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Analysis of Campylobacter spp. contamination in broilers from the farm to the final meat cuts by using restriction fragment length polymorphism of the polymerase chain reaction products

Cited by 33 publications

References 21 publications

The occurrence and sources of Campylobacter spp., Salmonellaenterica and Escherichia coli O157:H7 in the Salmon River, British Columbia, Canada

The occurrence and sources of Campylobacter spp., Salmonellaenterica and Escherichia coli O157:H7 in the Salmon River, British Columbia, Canada

Development of a Strain-Specific Molecular Method for Quantitating Individual Campylobacter Strains in Mixed Populations

Campylobacter spp. infection in humans and poultry

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