Analysis of c-Fos induction in response to social interaction in male and female Fisher 344 rats

Perkins, Amy E.; Woodruff, Elizabeth R.; Chun, Lauren E.; Spencer, Robert L.; Deak, Terrence

doi:10.1016/j.brainres.2017.07.022

Cited by 24 publications

(14 citation statements)

References 45 publications

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“…Here, although both males and females exhibited increased social interaction-induced c-fos expression after PSI, MJN110 significantly reduced social interaction-induced c-fos only in females. It has previously been reported that females engage in more social interaction than males (Perkins et al 2017), consistent with the current findings, and in that study social interaction-induced c-fos was greater in the mPFC of females than males (Perkins et al 2017). Sex differences in the effects of cannabinoid manipulations have also been previously reported.…”

Section: 0 Discussionsupporting

confidence: 93%

Monoacylglycerol lipase inhibition alters social behavior in male and female rats after post-weaning social isolation

Fontenot

Loetz

Ishiki

et al. 2018

Behavioural Brain Research

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Post-weaning social isolation (PSI) has been shown to increase aggressive behavior and alter medial prefrontal cortex (mPFC) function in rats. The present study sought to determine whether this phenotype would be normalized by increasing levels of the endocannabinoid 2-arachidonoylglycerol (2-AG) using pharmacological inhibition of monoacylglycerol lipase (MAGL). Male and female Sprague-Dawley rats were exposed to either 4 weeks of PSI or social rearing (SR) starting on postnatal day 21, then underwent a 15 min trial of social interaction with a novel, same-sex juvenile rat. Rats were administered an acute injection of the MAGL inhibitor MJN110 or vehicle prior to the social interaction. Rats received either 0 mg/kg (vehicle), 1 mg/kg, or 5 mg/kg of MJN110. Both doses of MJN110 decreased aggressive grooming, a measure of agonistic behavior, in both males and females, largely driven by decreased aggressive grooming in PSI rats. There were no effects of MJN110 on overall social behavior or play behavior, while modest effects were observed on locomotor activity in SR rats only. While social interaction increased c-Fos expression in the mPFC of both males and females, MJN110 reduced c-Fos preferentially in females. These results suggest that 2-AG can modulate specific social behaviors during adolescence, and may affect mPFC function differentially in males and females.

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Section: 0 Discussionsupporting

confidence: 93%

Monoacylglycerol lipase inhibition alters social behavior in male and female rats after post-weaning social isolation

Fontenot

Loetz

Ishiki

et al. 2018

Behavioural Brain Research

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“…Specifically, in Sprague Dawley rats, adult males exhibit greater levels of social behavior relative to females (Stack et al, 2010; Varlinskaya et al, 2014). In contrast, adult female F344 rats exhibit greater levels of social investigation relative to adult male F344 rats (Perkins et al, 2017). This is consistent with the hypothesis that increased number and/or activation of microglia, such as what was observed in the MEA here might adversely influence the expression of social behavior.…”

Section: 0 Discussionmentioning

confidence: 96%

“…The PVN receives substantial brainstem inputs and as such is a key site of integration of ascending signals and the neuroendocrine responses to stress (Myers et al, 2017; Pacak and Palkovits, 2001). The BNST is involved in social recognition (Dumais et al, 2016b) and exhibits sex-specific patterns of immediate early gene (c-Fos) activation following a social experience (Perkins et al, 2017). The amygdala has been implicated in social information processing in humans (Adolphs, 2003) and the MEA specifically exhibits induction of c-Fos in response to social interaction in adult rats that is blunted in aged rats (Salchner et al, 2004).…”

Section: 0 Introductionmentioning

confidence: 99%

Stereological Analysis of Microglia in Aged Male and Female Fischer 344 Rats in Socially Relevant Brain Regions

2018

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Aging is associated with a substantial decline in the expression of social behavior as well as increased neuroinflammation. Since immune activation and subsequent increased expression of cytokines can suppress social behavior in young rodents, we examined age and sex differences in microglia within brain regions critical to social behavior regulation (PVN, BNST, and MEA) as well as in the hippocampus. Adult (3-month) and aged (18-month) male and female F344 (N = 26, n = 5-8/group) rats were perfused and Iba-1 immunopositive microglia were assessed using unbiased stereology and optical density. For stereology, microglia were classified based on the following criteria: (1) thin ramified processes, (2) thick long processes, (3) stout processes, or (4) round/ameboid shape. Among the structures examined, the highest density of microglia was evident in the BNST and MEA. Aged rats of both sexes displayed increased total number of microglia number exclusively in the MEA. Sex differences also emerged, whereby aged females (but not males) displayed greater total number of microglia in the BNST relative to their young adult counterparts. When morphological features of microglia were assessed, aged rats exhibited increased soma size in the BNST, MEA, and CA3. Together, these findings provide a comprehensive characterization of microglia number and morphology under ambient conditions in CNS sites critical for the normal expression of social processes. To the extent that microglia morphology is predictive of reactivity and subsequent cytokine release, these data suggest that the expression of social behavior in late aging may be adversely influenced by heightened inflammation.

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“…Given our previous findings of age and/or sex differences in the social interaction test (Perkins et al, 2017, 2016), we first assessed social investigation and contact behavior exclusively in the saline vehicle group. Aging rats had significantly lower social investigation [F(1,37) = 38.44, p < 0.00001] and social contact behavior [F(1,37) = 11.05, p = 0.002] relative to their adult counterparts, whereas no sex differences were evident in either dependent measure (Figure 1 B).…”

Section: Resultsmentioning

confidence: 99%

“…Social interaction testing occurred in custom-made Plexiglas® chambers lined with clean pine shavings (45 × 30 × 30 cm). Each chamber was divided into two equally sized compartments separated by a clear Plexiglas® partition with an aperture (9 × 7 cm) that allowed the rats to move freely between compartments (Perkins et al, 2017). Males and females were tested in separate rooms with dim lighting (10–20 lux) between 0800 and 1300.…”

Section: Methodsmentioning

confidence: 99%

Late aging alters behavioral sensitivity to ethanol in a sex-specific manner in Fischer 344 rats

Perkins

Vore

Lovelock

2018

Pharmacology Biochemistry and Behavior

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Responsiveness to ethanol (EtOH) differs as a function of age. Adolescent rodents are less sensitive than adults to the sedative effects of EtOH, whereas they show enhanced sensitivity to EtOH-induced social facilitation. Late aging is associated with a natural decline in social behavior and aging-related peculiarities in sensitivity to EtOH have been largely unexplored. Whether there are sex differences in the behavioral response to EtOH during late aging remains unknown. Thus, behavioral responses to EtOH in male and female Fischer (F) 344 rats aged 4-5 months (adult) and 19-20 months (aging) were examined. First, the effects of saline and EtOH (0.5 and 0.75 g/kg) on social interaction were assessed. Social investigation and contact behavior were lower in aging animals and higher in females. Interestingly, in aged females, social contact behavior was increased following a 0.5 g/kg EtOH dose, whereas the same dose suppressed social contact in aged males. Behavioral sensitivity to the sedative effects of 3.0 and 3.5 g/kg EtOH was assessed with the loss of righting reflex (LORR) test. Although latency to LORR did not differ as a function of age or sex, aged rats showed significantly greater LORR duration and significantly lower blood ethanol concentrations (BECs) at regaining of the righting reflex relative to adults. In addition, females had a lower LORR duration, regardless of age; no sex differences were evident in BECs at awakening. In a second experiment, blood ethanol concentrations (BECs) over time were assessed following 0.75, 1.5, and 3.0 g/kg EtOH in 3-, 12-, and 18-month-old male and female F344 rats. Aged rats had higher peak BECs following 3.0 g/kg EtOH, whereas few age or sex differences were apparent at lower doses. Taken together, these data indicate that late aging is associated with altered sensitivity to the social facilitating effects and sedative effects of EtOH.

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Analysis of c-Fos induction in response to social interaction in male and female Fisher 344 rats

Cited by 24 publications

References 45 publications

Monoacylglycerol lipase inhibition alters social behavior in male and female rats after post-weaning social isolation

Monoacylglycerol lipase inhibition alters social behavior in male and female rats after post-weaning social isolation

Stereological Analysis of Microglia in Aged Male and Female Fischer 344 Rats in Socially Relevant Brain Regions

Late aging alters behavioral sensitivity to ethanol in a sex-specific manner in Fischer 344 rats

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