Analysis of Butylated Hydroxyanisole (Bha) in RBD Palm Oil and RBD Palm Olein Using Partial Least Squares Based on Ftir Spectroscopy

Abstract

A rapid method to determine butylated hydroxyanisole (BHA) in refined bleached and deodorized (RBD) palm oil and RBD palm olein was developed. The two sets of 50 stripped oils of RBD palm olein and RBD palm oil were spiked with known amounts of BHA concentrations up to 300 mg/kg (ppm) for calibration samples. The "leave-one-out" cross-validation procedure was used to verify the calibration model. Partial least squares (PLS) calibration models for predicting BHA were developed by using the Fourier transform infrared (FTIR) spectroscopy spectral regions from 3486 to 3140 cm -1 and 1140 to 898 cm -1 . The accuracy of the method was comparable to that of the high-performance liquid chromatography (HPLC), with coefficients of determination (R 2 ) of 0.9939 from calibration samples of RBD palm olein and an R 2 of 0.9884 for RBD palm oil samples. The results indicate that FTIR in conjunction with PLS is a useful analytical tool for simple and rapid quantitation of BHA in RBD palm oil and RBD palm olein for routine analysis.

Migration analysis, antioxidant, and mechanical characterization of polypropylene‐based active food packaging films loaded with BHA, BHT, and TBHQ

“…According to Ammawath et al. (2005) peak at 2963 cm −1 indicated an unsaturated = CH‒ (alkene, aromatic) bond. Wavenumbers 1597, 1484, and 1442 cm −1 were due to phenyl ring stretching and ‒C‒OH in plane bending (Mirghani & Che Man, 2003).…”

“…BHA spectrum (Figure 3B) exhibited peaks at 3395 and 2952 cm −1 related to vibration of ‒OH bounded to the phenol and –CH 2 , respectively (Roeges, 1994). The absorbance peaks at 1506 and 1414 cm −1 indicated phenyl ring and bending of the –CH 2 adjacent to the phenyl group, respectively (Ammawath, Che Man, Baharin, & Abdul Rahman, 2005). Peaks at 1365 and 1033 cm −1 corresponded to adjacent methyl and a strong stretching absorption of a C‒O bond, respectively (Mirghani, Man, Jinap, Baharin, & Bakar, 2001).…”

Functional and Antioxidant Properties of Plastic Bottle Caps Incorporated with BHA or BHT

“…Peaks at 2956 and 2872 cm −1 represented asymmetric and symmetric stretch vibrations of a methyl group, respectively. Peaks at 815, 765, and 679 cm −1 implied the presence of double-bonded carbon atoms (C = C) and in-plane phenol ring bending, while the band observed at 580 cm −1 indicated out-of-plane phenol ring bending [ 29 , 30 ].…”

CUPRAC total antioxidant capacity assay of lipophilic antioxidants in combination with hydrophilic antioxidants using the macrocyclic oligosaccharide methyl β-cyclodextrin as the solubility enhancer

“…2 shows the FT-IR spectra of solid inclusion complexes of BHA with M-b-CD obtained by various methods. The BHA spectrum exhibited peaks at 3393 cm À1 for -OH associated phenol, 2953 cm À1 assigned as vibration -CH 2 , 1507 cm À1 for phenyl ring, 1415 cm À1 for the bending of -CH 2 adjacent to the phenyl group, 1364 cm À1 for the adjacent -CH 3 , 1032 cm À1 for the strong stretching absorption of C-O, 817, 772, and 683 cm À1 bands for C@C out-of-plane ring [28]. Especially those characteristic bands of BHA in the region: 2000-500 cm À1 were obscured upon inclusion-complexation, either in kneaded or in freeze-dried products.…”